Characterization of coaggregation of Fusobacterium nucleatum PK1594 with six Porphyromonas gingivalis strains. 2009

Zvi Metzger, and Jaron Blasbalg, and Miri Dotan, and Igor Tsesis, and Ervin I Weiss
Department of Endodontology, The Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel. Metzger@post.tau.ac.il

Coaggregation is a key mechanism in biofilm formation. The aim of this study was to characterize the coaggregation of Fusobacterium nucleatum PK1594 with six Porphyromonas gingivalis strains in terms of kinetics and sugars inhibition. This coaggregation was quantitatively characterized by using a kinetic coaggregation assay. Sugar inhibition profiles were also quantitatively defined. Four types of interactions among these coaggregation partners were found: (1) fast coaggregation that was substantially inhibited by galactose, lactose, and fucose (strain PK1924); (2) fast coaggregation that was not inhibited by any of the sugars tested (strain 274); (3) slow coaggregation that was either substantially or partially inhibited by the sugars mentioned (strains HG405 and W50, respectively); and (4) strains that did not coaggregate with the fusobacteria (ATCC33277 and A7436). These results suggest that adhesin(s) other than the well-known galactose-mediated ones may be involved in coaggregation between F. nucleatum PK1594 and P. gingivalis strains.

UI MeSH Term Description Entries
D007785 Lactose A disaccharide of GLUCOSE and GALACTOSE in human and cow milk. It is used in pharmacy for tablets, in medicine as a nutrient, and in industry. Anhydrous Lactose,Lactose, Anhydrous
D005643 Fucose A six-member ring deoxysugar with the chemical formula C6H12O5. It lacks a hydroxyl group on the carbon at position 6 of the molecule. Deoxygalactose,alpha-Fucose,alpha Fucose
D005690 Galactose An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood. D-Galactose,Galactopyranose,Galactopyranoside,D Galactose
D001422 Bacterial Adhesion Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity. Adhesion, Bacterial,Adhesions, Bacterial,Bacterial Adhesions
D016966 Porphyromonas gingivalis A species of gram-negative, anaerobic, rod-shaped bacteria originally classified within the BACTEROIDES genus. This bacterium produces a cell-bound, oxygen-sensitive collagenase and is isolated from the human mouth. Bacteroides gingivalis
D016967 Fusobacterium nucleatum A species of gram-negative, anaerobic, rod-shaped bacteria isolated from the gingival margin and sulcus and from infections of the upper respiratory tract and pleural cavity.
D018441 Biofilms Encrustations formed from microbes (bacteria, algae, fungi, plankton, or protozoa) embedded in an EXTRACELLULAR POLYMERIC SUBSTANCE MATRIX that is secreted by the microbes. They occur on body surfaces such as teeth (DENTAL DEPOSITS); inanimate objects, and bodies of water. Biofilms are prevented from forming by treating surfaces with DENTIFRICES; DISINFECTANTS; ANTI-INFECTIVE AGENTS; and anti-fouling agents. Biofilm
D018829 Adhesins, Bacterial Cell-surface components or appendages of bacteria that facilitate adhesion (BACTERIAL ADHESION) to other cells or to inanimate surfaces. Most fimbriae (FIMBRIAE, BACTERIAL) of gram-negative bacteria function as adhesins, but in many cases it is a minor subunit protein at the tip of the fimbriae that is the actual adhesin. In gram-positive bacteria, a protein or polysaccharide surface layer serves as the specific adhesin. What is sometimes called polymeric adhesin (BIOFILMS) is distinct from protein adhesin. Adhesins, Fimbrial,Bacterial Adhesins,Fimbrial Adhesins,Adhesin, Bacterial,Bacterial Adhesin

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