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We have developed a simple and inexpensive method for largecapacity cAMP determination in biological materials. The assay is based on competitive binding of 3H-cAMP to proteins isolated from rabbit skeletal muscle. Bovine serum albumin is added to the incubate to reduce non-specific interference. Separation of free and bound radioactivity is performed by (NH4)2SO4 precipitation allowing determination of either free or bound fraction. In the latter case, all procedures are performed in the same tube, to which is finally added 1.2 ml of scintillation fluid for counting. By this only one fourth. The method has been applied to rat tissue extracts and urine with satisfactory sensitivity, precision and accuracy.
A Geisler, and
R Klysner, and
P Thams, and
S Christensen
November 1974,
Clinica chimica acta; international journal of clinical chemistry,
A Geisler, and
R Klysner, and
P Thams, and
S Christensen
April 1974,
Analytical biochemistry,
A Geisler, and
R Klysner, and
P Thams, and
S Christensen
March 1977,
Experientia,
A Geisler, and
R Klysner, and
P Thams, and
S Christensen
January 1972,
Advances in cyclic nucleotide research,
A Geisler, and
R Klysner, and
P Thams, and
S Christensen
November 1975,
Biochemical and biophysical research communications,
A Geisler, and
R Klysner, and
P Thams, and
S Christensen
May 1976,
Analytical biochemistry,
A Geisler, and
R Klysner, and
P Thams, and
S Christensen
August 1976,
Biochemical and biophysical research communications,
A Geisler, and
R Klysner, and
P Thams, and
S Christensen
March 1976,
Life sciences,
A Geisler, and
R Klysner, and
P Thams, and
S Christensen
June 1972,
Zeitschrift fur klinische Chemie und klinische Biochemie,
A Geisler, and
R Klysner, and
P Thams, and
S Christensen
July 1975,
Analytical biochemistry,
