BIOMAT Database Logo BIOMAT Database Logo

Evaluation of different wheat varieties by SDS-PAGE electrophoresis. 2007

Mohd Shuaib, and Ikhtiar Khan, and Zahir Ali, and Waqar Ali, and Taufiq Ahmad, and Alam Zeb
University of Malakand, Chakdara, Pakistan.

Wheat seed-storage proteins represent not only an important source of food and energy but it is also involved in the determination of bread-making quality. Wheat grains of thirteen wheat varieties were collected from different ecological regions of Pakistan. The variability of seed storage-proteins was analyzed by using SDS-PAGE electrophoresis. Electrophorogram for each variety was scored and presence or absence of each band noted and entered in a binary data matrix. Based on electrophoresis band spectra, Jaccard's Similarity Index (JSI) was calculated. Genetic diversity of wheat was evaluated by constructing the dendrogram for High Molecular Weight (HMW) and Low Molecular Weight (LMW) gluten subunit bands. It is concluded that seed storage protein profiles could be useful markers in the studies of genetic diversity and classification of adapted cultivars, thereby improving the efficiency of wheat breeding programs in cultivar development especially in developing countries like Pakistan.

UI MeSH Term Description Entries
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D004591 Electrophoresis, Polyacrylamide Gel Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D013045 Species Specificity The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species. Species Specificities,Specificities, Species,Specificity, Species
D014644 Genetic Variation Genotypic differences observed among individuals in a population. Genetic Diversity,Variation, Genetic,Diversity, Genetic,Diversities, Genetic,Genetic Diversities,Genetic Variations,Variations, Genetic
D014908 Triticum A plant genus of the family POACEAE that is the source of EDIBLE GRAIN. A hybrid with rye (SECALE CEREALE) is called TRITICALE. The seed is ground into FLOUR and used to make BREAD, and is the source of WHEAT GERM AGGLUTININS. Wheat,Durum Wheat,Triticum aestivum,Triticum durum,Triticum spelta,Triticum turgidum,Triticum turgidum subsp. durum,Triticum vulgare,Durum Wheats,Wheat, Durum
D016000 Cluster Analysis A set of statistical methods used to group variables or observations into strongly inter-related subgroups. In epidemiology, it may be used to analyze a closely grouped series of events or cases of disease or other health-related phenomenon with well-defined distribution patterns in relation to time or place or both. Clustering,Analyses, Cluster,Analysis, Cluster,Cluster Analyses,Clusterings

Related Publications

Mohd Shuaib, and Ikhtiar Khan, and Zahir Ali, and Waqar Ali, and Taufiq Ahmad, and Alam Zeb
Electrophoresis of Peptides (Tricine-SDS-PAGE).
June 2006, CSH protocols,
Mohd Shuaib, and Ikhtiar Khan, and Zahir Ali, and Waqar Ali, and Taufiq Ahmad, and Alam Zeb
One-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE).
January 2014, Methods in enzymology,
Mohd Shuaib, and Ikhtiar Khan, and Zahir Ali, and Waqar Ali, and Taufiq Ahmad, and Alam Zeb
Multiple SDS-PAGE on Vertical Electrophoresis Units.
June 2006, CSH protocols,
Mohd Shuaib, and Ikhtiar Khan, and Zahir Ali, and Waqar Ali, and Taufiq Ahmad, and Alam Zeb
[Preparative 2-dimensional electrophoresis--slab NEPHGE/SDS-PAGE].
June 1992, Seikagaku. The Journal of Japanese Biochemical Society,
Mohd Shuaib, and Ikhtiar Khan, and Zahir Ali, and Waqar Ali, and Taufiq Ahmad, and Alam Zeb
Evaluation of patterns of urinary proteins by SDS-PAGE in rats of different ages.
May 1996, Mechanisms of ageing and development,
Mohd Shuaib, and Ikhtiar Khan, and Zahir Ali, and Waqar Ali, and Taufiq Ahmad, and Alam Zeb
Removal of small proteins--the stepchild of dialysis therapy. Protein permeability of different hemofiltration membranes analysed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE).
January 1987, Advances in experimental medicine and biology,
Mohd Shuaib, and Ikhtiar Khan, and Zahir Ali, and Waqar Ali, and Taufiq Ahmad, and Alam Zeb
Detection of molecular weight of PMCA isoform with 20 cm SDS PAGE electrophoresis: compared with 8 cm SDS PAGE.
September 2005, Yan ke xue bao = Eye science,
Mohd Shuaib, and Ikhtiar Khan, and Zahir Ali, and Waqar Ali, and Taufiq Ahmad, and Alam Zeb
Two-dimensional gel electrophoresis: glass tube-based IEF followed by SDS-PAGE.
January 2012, Methods in molecular biology (Clifton, N.J.),
Mohd Shuaib, and Ikhtiar Khan, and Zahir Ali, and Waqar Ali, and Taufiq Ahmad, and Alam Zeb
Two-Dimensional Gel Electrophoresis by Glass Tube-Based IEF and SDS-PAGE.
January 2019, Methods in molecular biology (Clifton, N.J.),
Mohd Shuaib, and Ikhtiar Khan, and Zahir Ali, and Waqar Ali, and Taufiq Ahmad, and Alam Zeb
[Determination of human fetal lens protein with gradient SDS-polyacrylamide gel electrophoresis (SDS-PAGE)].
September 1988, Yan ke xue bao = Eye science,
Copied contents to your clipboard!