Biomaterial Database

[Establishment of a podocyte cell injury model induced by puromycin aminonucleoside]. 2008

Shu Fang Liu, and Jie Ding, and Qing Feng Fan, and Han Zhang

Department of Pediatrics, Peking University First Hospital, Beijing, China.

OBJECTIVE To establish a podocyte cell injury model induced by puromycin aminonucleoside (PAN), an in vitro model for studying the role of podocytes, especial the slit diaphragm molecules in proteinuria at the cellular and molecular levels. METHODS MPC5 were treated for 24 and 48 hours by 15, 45 and 75 mg/L PAN, respectively. The podocyte molecular behavior during podocyte injury was evaluated: the apoptotic podocyte cells were revealed with FITC-Annexin V and Propidium Iodide (PI) assay and the proliferative podocyte cells detected with MTT assay after PAN treatment. The distribution of Nephrin and Podocin was revealed with indirect-immunofluorescent staining under confocal microscope. The distribution of F-actin was revealed with direct-immunofluorescent staining under microscope. RESULTS The percentage of apoptotic podocyte cells was increased in a dose- and time-dependent manner after PAN treatment. In PAN-treated group, the apoptosis was obviously increased at hour 48, the PAN-45 treated group was 33.48% +/- 14.55% and PAN-75 treated group 38.01% +/- 12.13% vs the control group 6.38% +/- 0.50% (P<0.01). CONCLUSIONS We set up an in vitro podocyte injury model treated with PAN for the first time. This reliable cell model is a good basis for further studies on podocyte injury.

UI	MeSH Term	Description	Entries
D011692	Puromycin Aminonucleoside	PUROMYCIN derivative that lacks the methoxyphenylalanyl group on the amine of the sugar ring. It is an antibiotic with antineoplastic properties and can cause nephrosis.	3'-Amino-3'-deoxy-N,N-dimethyladenosine,Aminonucleoside,3' Amino 3' deoxy N,N dimethyladenosine,Aminonucleoside, Puromycin
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D017209	Apoptosis	A regulated cell death mechanism characterized by distinctive morphologic changes in the nucleus and cytoplasm, including the endonucleolytic cleavage of genomic DNA, at regularly spaced, internucleosomal sites, i.e., DNA FRAGMENTATION. It is genetically programmed and serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.	Apoptosis, Extrinsic Pathway,Apoptosis, Intrinsic Pathway,Caspase-Dependent Apoptosis,Classic Apoptosis,Classical Apoptosis,Programmed Cell Death,Programmed Cell Death, Type I,Apoptoses, Extrinsic Pathway,Apoptoses, Intrinsic Pathway,Apoptosis, Caspase-Dependent,Apoptosis, Classic,Apoptosis, Classical,Caspase Dependent Apoptosis,Cell Death, Programmed,Classic Apoptoses,Extrinsic Pathway Apoptoses,Extrinsic Pathway Apoptosis,Intrinsic Pathway Apoptoses,Intrinsic Pathway Apoptosis
D050199	Podocytes	Highly differentiated epithelial cells of the visceral layer of BOWMAN CAPSULE of the KIDNEY. They are composed of a cell body with major CELL SURFACE EXTENSIONS and secondary fingerlike extensions called pedicels. They enwrap the KIDNEY GLOMERULUS capillaries with their cell surface extensions forming a filtration structure. The pedicels of neighboring podocytes interdigitate with each other leaving between them filtration slits that are bridged by an extracellular structure impermeable to large macromolecules called the slit diaphragm, and provide the last barrier to protein loss in the KIDNEY.	Visceral Epithelial Cells, Glomerular,Epithelial Cells, Glomerular Visceral,Glomerular Visceral Epithelial Cells,Podocyte
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D023421	Models, Animal	Non-human animals, selected because of specific characteristics, for use in experimental research, teaching, or testing.	Experimental Animal Models,Laboratory Animal Models,Animal Model,Animal Model, Experimental,Animal Model, Laboratory,Animal Models,Animal Models, Experimental,Animal Models, Laboratory,Experimental Animal Model,Laboratory Animal Model,Model, Animal,Model, Experimental Animal,Model, Laboratory Animal,Models, Experimental Animal,Models, Laboratory Animal

Related Publications

Shu Fang Liu, and Jie Ding, and Qing Feng Fan, and Han Zhang

The role of survivin in podocyte injury induced by puromycin aminonucleoside.

April 2014, International journal of molecular sciences,

Shu Fang Liu, and Jie Ding, and Qing Feng Fan, and Han Zhang

The role of PTEN in puromycin aminonucleoside-induced podocyte injury.

January 2022, International journal of medical sciences,

Shu Fang Liu, and Jie Ding, and Qing Feng Fan, and Han Zhang

Effect of TRPC6 knockdown on puromycin aminonucleoside-induced podocyte injury.

June 2012, Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban,

Shu Fang Liu, and Jie Ding, and Qing Feng Fan, and Han Zhang

Puromycin aminonucleoside-induced podocyte injury is ameliorated by the Smad3 inhibitor SIS3.

August 2020, FEBS open bio,

Shu Fang Liu, and Jie Ding, and Qing Feng Fan, and Han Zhang

Calpain-Mediated Cleavage of Calcineurin in Puromycin Aminonucleoside-Induced Podocyte Injury.

January 2016, PloS one,

Shu Fang Liu, and Jie Ding, and Qing Feng Fan, and Han Zhang

Inhibition of microRNA-130b Alleviated Podocyte Injury Induced by Puromycin Aminonucleoside in vitro.

May 2021, Annals of clinical and laboratory science,

Shu Fang Liu, and Jie Ding, and Qing Feng Fan, and Han Zhang

Role of bad in podocyte apoptosis induced by puromycin aminonucleoside.

March 2013, Transplantation proceedings,