-transparent.png){kind=logo}
Using Bacteriophage lambda and plasmid pAT153 and pNQ122 as vectors, alpha-Amylase gene from B. megaterium has been cloned into both hosts of E. coli and B. subtilis. Expression level of the gene is 250 times higher than B. megaterium when it resides in B. subtilis. The enzyme produced by B. subtilis harboring the hybrid plasmid can digest amylase into maltose and maltotriose at first, then turn them to maltose and glucose, as incubation time extended. It also can digest maltotriose to maltose and glucose. As a control, the extracts from the broth of recipient strain have no detectable amylase activities. Therefore the enzyme coded by this gene is defined as saccharifying type alpha-amylase. Its molecular weight is about 58,000 daltons.
X Y Lü, and
R Z Jiang, and
G F Wang
January 1982,
Gene,
X Y Lü, and
R Z Jiang, and
G F Wang
November 1983,
Applied and environmental microbiology,
X Y Lü, and
R Z Jiang, and
G F Wang
June 1991,
Molekuliarnaia genetika, mikrobiologiia i virusologiia,
X Y Lü, and
R Z Jiang, and
G F Wang
September 1983,
Gene,
X Y Lü, and
R Z Jiang, and
G F Wang
April 1992,
Biochemical and biophysical research communications,
X Y Lü, and
R Z Jiang, and
G F Wang
October 1983,
Journal of bacteriology,
X Y Lü, and
R Z Jiang, and
G F Wang
August 1992,
Wei sheng wu xue bao = Acta microbiologica Sinica,
X Y Lü, and
R Z Jiang, and
G F Wang
December 1979,
Biochemical and biophysical research communications,
X Y Lü, and
R Z Jiang, and
G F Wang
June 1998,
Bioscience, biotechnology, and biochemistry,
X Y Lü, and
R Z Jiang, and
G F Wang
May 2002,
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica,
