Biomaterial Database

[Comparative characteristics of lactate dehydrogenase from the liver and white muscles of common carp (Cyprinus carpio)]. 2008

O I Kubrak, and O V Lushchak, and V I Lushchak

Lactate dehydrogenase from the liver and white muscles of common carp (Cyprinus carpio) was purified by the differential precipitation of supernatants with ammonium sulfate. The results of native electrophoresis of partially purified lactate degydrogenase (LDH) and LDH from some tissues exhibited the different isozyme composition there. However, the kinetic properties of these isozymes from two tissues were not different, except for the value of K(m) for pyruvate, which was 72.5 +/- 11 and 217 +/- 32 microM for the liver and muscle, respectively. LDH from the liver and white muscle had similar pH-dependences with pH-optima for the both LDH activities between 6.75 and 8.00. But studied isozymes demonstrated different thermostability. The partially purified LDH from the liver showed 450% activity after 2 h at 50 degrees C. In contrast, the partially purified LDH from the muscle exhibited about 300% activity after 3 h at 65 degrees C. The heating of partially purified LDH from the muscle caused complete purification of this isozyme.

UI	MeSH Term	Description	Entries
D007527	Isoenzymes	Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.	Alloenzyme,Allozyme,Isoenzyme,Isozyme,Isozymes,Alloenzymes,Allozymes
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D002347	Carps	Common name for a number of different species of fish in the family Cyprinidae. This includes, among others, the common carp, crucian carp, grass carp, and silver carp.	Carassius carassius,Crucian Carp,Cyprinus,Grass Carp,Carp,Ctenopharyngodon idellus,Cyprinus carpio,Hypophthalmichthys molitrix,Koi Carp,Silver Carp,Carp, Crucian,Carp, Grass,Carp, Koi,Carp, Silver,Carps, Crucian,Carps, Grass,Carps, Silver,Crucian Carps,Grass Carps,Silver Carps
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000645	Ammonium Sulfate	Sulfuric acid diammonium salt. It is used in CHEMICAL FRACTIONATION of proteins.	Sulfate, Ammonium
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D045022	Lactate Dehydrogenases	Alcohol oxidoreductases with substrate specificity for LACTIC ACID.	Dehydrogenases, Lactate
D018482	Muscle, Skeletal	A subtype of striated muscle, attached by TENDONS to the SKELETON. Skeletal muscles are innervated and their movement can be consciously controlled. They are also called voluntary muscles.	Anterior Tibial Muscle,Gastrocnemius Muscle,Muscle, Voluntary,Plantaris Muscle,Skeletal Muscle,Soleus Muscle,Muscle, Anterior Tibial,Muscle, Gastrocnemius,Muscle, Plantaris,Muscle, Soleus,Muscles, Skeletal,Muscles, Voluntary,Skeletal Muscles,Tibial Muscle, Anterior,Voluntary Muscle,Voluntary Muscles