BIOMAT Database Logo BIOMAT Database Logo

Determination of ampicillin in biological fluids by coupled-column liquid chromatography and post-column derivatization. 1991

K Lanbeck-Vallén, and J Carlqvist, and T Nordgren
Department of Bioanalysis, Astra Research Centre AB, Södertälje, Sweden.

A rapid and sensitive high-performance liquid chromatographic method has been developed for the determination of ampicillin in plasma and urine. The method involves a simple deproteinization step and separation on a coupled-column chromatographic system followed by post-column derivatization and fluorescence detection. The method has been used for the determination of ampicillin in various clinical studies. The high sensitivity makes it especially useful for small sample volumes, e.g. samples from pediatric patients.

UI MeSH Term Description Entries
D002648 Child A person 6 to 12 years of age. An individual 2 to 5 years old is CHILD, PRESCHOOL. Children
D002853 Chromatography, Liquid Chromatographic techniques in which the mobile phase is a liquid. Liquid Chromatography
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000667 Ampicillin Semi-synthetic derivative of penicillin that functions as an orally active broad-spectrum antibiotic. Penicillin, Aminobenzyl,Amcill,Aminobenzylpenicillin,Ampicillin Sodium,Ampicillin Trihydrate,Antibiotic KS-R1,Omnipen,Pentrexyl,Polycillin,Ukapen,Aminobenzyl Penicillin,Antibiotic KS R1,KS-R1, Antibiotic,Sodium, Ampicillin,Trihydrate, Ampicillin
D013056 Spectrophotometry, Ultraviolet Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Ultraviolet Spectrophotometry

Related Publications

K Lanbeck-Vallén, and J Carlqvist, and T Nordgren
Determination of amoxicillin in body fluids by reversed-phase liquid chromatography coupled with a post-column derivatization procedure.
November 1979, Journal of chromatography,
K Lanbeck-Vallén, and J Carlqvist, and T Nordgren
[Determination of 14 sulfonamide residues in shrimps by high performance liquid chromatography coupled with post-column derivatization].
August 2014, Se pu = Chinese journal of chromatography,
K Lanbeck-Vallén, and J Carlqvist, and T Nordgren
Simultaneous determination of ampicillin and metampicillin in biological fluids using high-performance liquid chromatography with column switching.
February 1995, Journal of chromatography. B, Biomedical applications,
K Lanbeck-Vallén, and J Carlqvist, and T Nordgren
Determination of isofezolac in biological fluids by reversed-phase liquid column chromatography.
June 1980, Journal of chromatography,
K Lanbeck-Vallén, and J Carlqvist, and T Nordgren
Determination of metformin in biological fluids by derivatization followed by high-performance liquid chromatography.
March 1977, Journal of chromatography,
K Lanbeck-Vallén, and J Carlqvist, and T Nordgren
Determination of BAY y 3118, a novel 4-quinolone, in biological fluids using high-performance liquid chromatography and photothermal post-column derivatization.
June 1993, Journal of chromatography,
K Lanbeck-Vallén, and J Carlqvist, and T Nordgren
Automated determination of amoxycillin in biological fluids by column switching in ion-pair reversed-phase liquid chromatographic systems with post-column derivatization.
November 1985, Journal of chromatography,
K Lanbeck-Vallén, and J Carlqvist, and T Nordgren
Determination of N-acetylcysteine, intact and oxidized, in plasma by column liquid chromatography and post-column derivatization.
January 1987, Journal of chromatography,
K Lanbeck-Vallén, and J Carlqvist, and T Nordgren
Determination of citrulline and homocitrulline by high-performance liquid chromatography with post-column derivatization.
October 1990, Journal of chromatography,
K Lanbeck-Vallén, and J Carlqvist, and T Nordgren
Fluorimetric determination of pantothenic acid in foods by liquid chromatography with post-column derivatization.
April 2004, Journal of chromatography. A,
Copied contents to your clipboard!