BIOMAT Database Logo BIOMAT Database Logo

Purification and characterization of L-phenylalanine aminopeptidase from chick-pea cotyledons (Cicer arietinum L.). 2009

Margarita Marinova, and Alexander Dolashki, and Florian Altenberend, and Stefan Stevanovic, and Wolfgang Voelter, and Bozhidar Tchorbanov
Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. 9, 1113 Sofia, Bulgaria.

Chick-pea (Cicer arietinum L.) cotyledons are unique source of aminopeptidase - 8-9 U/g cotyledons was observed using L-leucine-p-nitroanilide as substrate. The aminopeptidase was purified (65 kDa, pI 4.8 ) reaching a specific activity of 220 U/mg at pH 7.0-7.2 and 35-40 degrees C. The determined constant of specificity k(cat)/K(m) during hydrolysis of N-unsubstituted amino acid-p-nitroanilides showed a decrease order: Phe>Leu>Pro>Ile>Val>Ala. The enzyme was strongly inhibited by p-chloromercuribenzoic acid as well as in a competitive rate by the antihypertensive peptides Ile-Pro-Pro and Val-Pro-Pro.

UI MeSH Term Description Entries
D009842 Oligopeptides Peptides composed of between two and twelve amino acids. Oligopeptide
D006863 Hydrogen-Ion Concentration The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000626 Aminopeptidases A subclass of EXOPEPTIDASES that act on the free N terminus end of a polypeptide liberating a single amino acid residue. EC 3.4.11. Aminopeptidase
D013379 Substrate Specificity A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts. Specificities, Substrate,Specificity, Substrate,Substrate Specificities
D013696 Temperature The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms. Temperatures
D016415 Sequence Alignment The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms. Sequence Homology Determination,Determination, Sequence Homology,Alignment, Sequence,Alignments, Sequence,Determinations, Sequence Homology,Sequence Alignments,Sequence Homology Determinations
D018548 Cotyledon A part of the embryo in a seed plant. The number of cotyledons is an important feature in classifying plants. In seeds without an endosperm, they store food which is used in germination. In some plants, they emerge above the soil surface and become the first photosynthetic leaves. (From Concise Dictionary of Biology, 1990) Coleoptile,Seed Leaf,Coleoptiles,Cotyledons,Seed Leaves,Leaf, Seed,Leave, Seed,Leaves, Seed,Seed Leave
D029863 Cicer A plant genus of the family FABACEAE known for the edible beans. Chickpea,Garbanzo,Cicer arietinum,Chickpeas,Cicer arietinums,Cicers,Garbanzos,arietinum, Cicer

Related Publications

Margarita Marinova, and Alexander Dolashki, and Florian Altenberend, and Stefan Stevanovic, and Wolfgang Voelter, and Bozhidar Tchorbanov
Purification and characterization of a membrane-bound ATP diphosphohydrolase from Cicer arietinum (chick-pea)roots.
September 1981, The Biochemical journal,
Margarita Marinova, and Alexander Dolashki, and Florian Altenberend, and Stefan Stevanovic, and Wolfgang Voelter, and Bozhidar Tchorbanov
UDP-glucose:isoflavone 7-O-glucosyltransferase from roots of chick pea (Cicer arietinum L.).
November 1981, Archives of biochemistry and biophysics,
Margarita Marinova, and Alexander Dolashki, and Florian Altenberend, and Stefan Stevanovic, and Wolfgang Voelter, and Bozhidar Tchorbanov
Plant regeneration via somatic embryogenesis in chick pea (Cicer arietinum L.).
September 1993, Plant cell reports,
Margarita Marinova, and Alexander Dolashki, and Florian Altenberend, and Stefan Stevanovic, and Wolfgang Voelter, and Bozhidar Tchorbanov
Characterization of beta-glucosidase isoenzymes possibly involved in lignification from chick pea (Cicer arietinum L.) cell suspension cultures.
March 1978, European journal of biochemistry,
Margarita Marinova, and Alexander Dolashki, and Florian Altenberend, and Stefan Stevanovic, and Wolfgang Voelter, and Bozhidar Tchorbanov
A β-galactosidase from chick pea (Cicer arietinum) seeds: its purification, biochemical properties and industrial applications.
September 2012, Food chemistry,
Margarita Marinova, and Alexander Dolashki, and Florian Altenberend, and Stefan Stevanovic, and Wolfgang Voelter, and Bozhidar Tchorbanov
Ciceribacter lividus gen. nov., sp. nov., isolated from rhizosphere soil of chick pea (Cicer arietinum L.).
December 2013, International journal of systematic and evolutionary microbiology,
Margarita Marinova, and Alexander Dolashki, and Florian Altenberend, and Stefan Stevanovic, and Wolfgang Voelter, and Bozhidar Tchorbanov
Effect of acetyl esterification on physicochemical properties of chick pea (Cicer arietinum L.) starch.
July 2015, Journal of food science and technology,
Margarita Marinova, and Alexander Dolashki, and Florian Altenberend, and Stefan Stevanovic, and Wolfgang Voelter, and Bozhidar Tchorbanov
Nutritional evaluation of sausages containing chick pea flour (Cicer arietinum).
January 1985, Meat science,
Margarita Marinova, and Alexander Dolashki, and Florian Altenberend, and Stefan Stevanovic, and Wolfgang Voelter, and Bozhidar Tchorbanov
A novel superoxide dismutase from Cicer arietinum L. seedlings: isolation, purification and characterization.
July 2013, Protein and peptide letters,
Margarita Marinova, and Alexander Dolashki, and Florian Altenberend, and Stefan Stevanovic, and Wolfgang Voelter, and Bozhidar Tchorbanov
Malonyl-coenzyme A:isoflavone 7-O-glucoside-6"-O-malonyltransferase from roots of chick pea (Cicer arietinum L.).
November 1984, Archives of biochemistry and biophysics,
Copied contents to your clipboard!