Abstract Hydrin 2 (vasotocinyl-Gly), found along with vasotocin in the neurohypophysis of frogs and toads but not of other vasotocin-bearers such as birds and reptiles, is believed to act on water permeability of frog skin, whereas vasotocin mainly fulfils the antidiuretic function on the kidney. In order to understand the peculiar regulation of provasotocin differential processing in amphibians, conversion of hydrin 2 into vasotocin has been attempted using bovine pituitary granule alpha-amidating enzyme. Generated vasotocin has pharmacological properties and Chromatographic behaviour in high-performance liquid chromatography identical to those of synthetic vasotocin. However, the low yield of conversion (5% to 10%) suggests that additional factor(s) might be involved in the physiological processing.