BIOMAT Database Logo BIOMAT Database Logo

Isolation of osteogenic progenitors from human amniotic fluid using a single step culture protocol. 2009

Ivana Antonucci, and Irene Iezzi, and Elisena Morizio, and Filiberto Mastrangelo, and Andrea Pantalone, and Monica Mattioli-Belmonte, and Antonio Gigante, and Vincenzo Salini, and Giuseppe Calabrese, and Stefano Tetè, and Giandomenico Palka, and Liborio Stuppia
Department of Biomedical Sciences, G, d'Annunzio University, Chieti-Pescara, Italy. antonucciivana@libero.it

BACKGROUND Stem cells isolated from amniotic fluid are known to be able to differentiate into different cells types, being thus considered as a potential tool for cellular therapy of different human diseases. In the present study, we report a novel single step protocol for the osteoblastic differentiation of human amniotic fluid cells. RESULTS The described protocol is able to provide osteoblastic cells producing nodules of calcium mineralization within 18 days from withdrawal of amniotic fluid samples. These cells display a complete expression of osteogenic markers (COL1, ONC, OPN, OCN, OPG, BSP, Runx2) within 30 days from withdrawal. In order to test the ability of these cells to proliferate on surfaces commonly used in oral osteointegrated implantology, we carried out cultures onto different test disks, namely smooth copper, machined titanium and Sandblasted and Acid Etching titanium (SLA titanium). Electron microscopy analysis evidenced the best cell growth on this latter surface. CONCLUSIONS The described protocol provides an efficient and time-saving tool for the production of osteogenic cells from amniotic fluid that in the future could be used in oral osteointegrated implantology.

UI MeSH Term Description Entries
D010006 Osteoblasts Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone. Osteoblast
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000653 Amniotic Fluid A clear, yellowish liquid that envelopes the FETUS inside the sac of AMNION. In the first trimester, it is likely a transudate of maternal or fetal plasma. In the second trimester, amniotic fluid derives primarily from fetal lung and kidney. Cells or substances in this fluid can be removed for prenatal diagnostic tests (AMNIOCENTESIS). Amniotic Fluid Index,Amniotic Fluid Indices,Amniotic Fluids,Fluid Index, Amniotic,Fluid Indices, Amniotic,Fluid, Amniotic,Fluids, Amniotic,Index, Amniotic Fluid,Indices, Amniotic Fluid
D015415 Biomarkers Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, ENVIRONMENTAL EXPOSURE and its effects, disease diagnosis; METABOLIC PROCESSES; SUBSTANCE ABUSE; PREGNANCY; cell line development; EPIDEMIOLOGIC STUDIES; etc. Biochemical Markers,Biological Markers,Biomarker,Clinical Markers,Immunologic Markers,Laboratory Markers,Markers, Biochemical,Markers, Biological,Markers, Clinical,Markers, Immunologic,Markers, Laboratory,Markers, Serum,Markers, Surrogate,Markers, Viral,Serum Markers,Surrogate Markers,Viral Markers,Biochemical Marker,Biologic Marker,Biologic Markers,Clinical Marker,Immune Marker,Immune Markers,Immunologic Marker,Laboratory Marker,Marker, Biochemical,Marker, Biological,Marker, Clinical,Marker, Immunologic,Marker, Laboratory,Marker, Serum,Marker, Surrogate,Serum Marker,Surrogate End Point,Surrogate End Points,Surrogate Endpoint,Surrogate Endpoints,Surrogate Marker,Viral Marker,Biological Marker,End Point, Surrogate,End Points, Surrogate,Endpoint, Surrogate,Endpoints, Surrogate,Marker, Biologic,Marker, Immune,Marker, Viral,Markers, Biologic,Markers, Immune
D059630 Mesenchymal Stem Cells Mesenchymal stem cells, also referred to as multipotent stromal cells or mesenchymal stromal cells are multipotent, non-hematopoietic adult stem cells that are present in multiple tissues, including BONE MARROW; ADIPOSE TISSUE; and WHARTON JELLY. Mesenchymal stem cells can differentiate into mesodermal lineages, such as adipocytic, osteocytic and chondrocytic. Adipose Tissue-Derived Mesenchymal Stem Cell,Adipose Tissue-Derived Mesenchymal Stromal Cell,Adipose-Derived Mesenchymal Stem Cell,Bone Marrow Mesenchymal Stem Cell,Mesenchymal Stromal Cell,Mesenchymal Stromal Cells,Multipotent Bone Marrow Stromal Cell,Multipotent Mesenchymal Stromal Cell,Adipose Tissue-Derived Mesenchymal Stem Cells,Adipose Tissue-Derived Mesenchymal Stromal Cells,Adipose-Derived Mesenchymal Stem Cells,Adipose-Derived Mesenchymal Stromal Cells,Bone Marrow Mesenchymal Stem Cells,Bone Marrow Stromal Cell,Bone Marrow Stromal Cells,Bone Marrow Stromal Cells, Multipotent,Bone Marrow Stromal Stem Cells,Mesenchymal Progenitor Cell,Mesenchymal Progenitor Cells,Mesenchymal Stem Cell,Mesenchymal Stem Cells, Adipose-Derived,Mesenchymal Stromal Cells, Multipotent,Multipotent Bone Marrow Stromal Cells,Multipotent Mesenchymal Stromal Cells,Stem Cells, Mesenchymal,Wharton Jelly Cells,Wharton's Jelly Cells,Adipose Derived Mesenchymal Stem Cell,Adipose Derived Mesenchymal Stem Cells,Adipose Derived Mesenchymal Stromal Cells,Adipose Tissue Derived Mesenchymal Stem Cell,Adipose Tissue Derived Mesenchymal Stem Cells,Adipose Tissue Derived Mesenchymal Stromal Cell,Adipose Tissue Derived Mesenchymal Stromal Cells,Mesenchymal Stem Cells, Adipose Derived,Progenitor Cell, Mesenchymal,Progenitor Cells, Mesenchymal,Stem Cell, Mesenchymal,Stromal Cell, Mesenchymal,Stromal Cells, Mesenchymal,Wharton's Jelly Cell,Whartons Jelly Cells
D018929 Cell Culture Techniques Methods for maintaining or growing CELLS in vitro. Cell Culture,Cell Culture Technique,Cell Cultures,Culture Technique, Cell,Culture Techniques, Cell

Related Publications

Ivana Antonucci, and Irene Iezzi, and Elisena Morizio, and Filiberto Mastrangelo, and Andrea Pantalone, and Monica Mattioli-Belmonte, and Antonio Gigante, and Vincenzo Salini, and Giuseppe Calabrese, and Stefano Tetè, and Giandomenico Palka, and Liborio Stuppia
Isolation of human multipotent mesenchymal stem cells from second-trimester amniotic fluid using a novel two-stage culture protocol.
June 2004, Human reproduction (Oxford, England),
Ivana Antonucci, and Irene Iezzi, and Elisena Morizio, and Filiberto Mastrangelo, and Andrea Pantalone, and Monica Mattioli-Belmonte, and Antonio Gigante, and Vincenzo Salini, and Giuseppe Calabrese, and Stefano Tetè, and Giandomenico Palka, and Liborio Stuppia
Isolation of prolactin from human amniotic fluid.
May 1977, Biochimica et biophysica acta,
Ivana Antonucci, and Irene Iezzi, and Elisena Morizio, and Filiberto Mastrangelo, and Andrea Pantalone, and Monica Mattioli-Belmonte, and Antonio Gigante, and Vincenzo Salini, and Giuseppe Calabrese, and Stefano Tetè, and Giandomenico Palka, and Liborio Stuppia
Isolation of human surfactant protein A from amniotic fluid.
December 1995, The Malaysian journal of pathology,
Ivana Antonucci, and Irene Iezzi, and Elisena Morizio, and Filiberto Mastrangelo, and Andrea Pantalone, and Monica Mattioli-Belmonte, and Antonio Gigante, and Vincenzo Salini, and Giuseppe Calabrese, and Stefano Tetè, and Giandomenico Palka, and Liborio Stuppia
[Isolation of human pluripotent mesenchymal stem cells from second-trimester amniotic fluid using two kinds of culture protocol and their differentiation into neuron-like cells].
December 2009, Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue,
Ivana Antonucci, and Irene Iezzi, and Elisena Morizio, and Filiberto Mastrangelo, and Andrea Pantalone, and Monica Mattioli-Belmonte, and Antonio Gigante, and Vincenzo Salini, and Giuseppe Calabrese, and Stefano Tetè, and Giandomenico Palka, and Liborio Stuppia
Isolation of isohormones of human prolactin from amniotic fluid.
January 1974, Endocrine research communications,
Ivana Antonucci, and Irene Iezzi, and Elisena Morizio, and Filiberto Mastrangelo, and Andrea Pantalone, and Monica Mattioli-Belmonte, and Antonio Gigante, and Vincenzo Salini, and Giuseppe Calabrese, and Stefano Tetè, and Giandomenico Palka, and Liborio Stuppia
Isolation and culture of osteoblast progenitors from human fetal calvarium.
December 1994, Transplantation proceedings,
Ivana Antonucci, and Irene Iezzi, and Elisena Morizio, and Filiberto Mastrangelo, and Andrea Pantalone, and Monica Mattioli-Belmonte, and Antonio Gigante, and Vincenzo Salini, and Giuseppe Calabrese, and Stefano Tetè, and Giandomenico Palka, and Liborio Stuppia
Isolation and characterization of a pulmonary glycoprotein from human amniotic fluid.
December 1978, Biochimica et biophysica acta,
Ivana Antonucci, and Irene Iezzi, and Elisena Morizio, and Filiberto Mastrangelo, and Andrea Pantalone, and Monica Mattioli-Belmonte, and Antonio Gigante, and Vincenzo Salini, and Giuseppe Calabrese, and Stefano Tetè, and Giandomenico Palka, and Liborio Stuppia
Isolation of biliverdin from amniotic fluid.
March 1969, Clinica chimica acta; international journal of clinical chemistry,
Ivana Antonucci, and Irene Iezzi, and Elisena Morizio, and Filiberto Mastrangelo, and Andrea Pantalone, and Monica Mattioli-Belmonte, and Antonio Gigante, and Vincenzo Salini, and Giuseppe Calabrese, and Stefano Tetè, and Giandomenico Palka, and Liborio Stuppia
Isolation & chemical composition of lung surfactant from human amniotic fluid.
February 1994, The Indian journal of medical research,
Ivana Antonucci, and Irene Iezzi, and Elisena Morizio, and Filiberto Mastrangelo, and Andrea Pantalone, and Monica Mattioli-Belmonte, and Antonio Gigante, and Vincenzo Salini, and Giuseppe Calabrese, and Stefano Tetè, and Giandomenico Palka, and Liborio Stuppia
Isolation and quantitation of steroids from normal human amniotic fluid.
August 1968, The Journal of clinical endocrinology and metabolism,
Copied contents to your clipboard!