The results of a previous experiment have shown that stereothreshold varies as a function of the luminance difference between a target and its background. When the luminance of the target is the same as that of the background (isoluminance) the stereothreshold was elevated by a factor of 3 as compared with a situation where there was maximum luminance difference between the target and the background. Generally, if the target and the background have the same color, stereothreshold at a particular luminance difference level was lower (stereoacuity better) than if the target and the background have different colors. This indicates a possible effect of chromatic aberration on stereothreshold which could be responsible for the 3-fold increase in threshold at isoluminance. This possibility has been investigated in this experiment. The results show that even though stereothresholds are sensitive to chromatic aberration this factor cannot explain the elevated stereothreshold at isoluminance.