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Cytochrome c immobilized on cyanogen bromide-activated Sepharose 4B may be used to study photochemical reactions in chloroplasts. Chloroplast reduction of both immobilized and soluble forms of the cytochrome occurs along the exogenous and endogenous pathways which results in a weaker reduction of the immobilized protein as compared to that of the soluble one. The time of the reduced immobilized cytochrome c oxidation in the dark is two orders of magnitude greater than that of the soluble one. This fact may be interpreted in terms of spatial dissociation of reductase and oxidase centers of chloroplasts with reference to the cytochrome. The optimal ionic strength for cytochrome reduction, i.e. ionic strength causing an in vitro destruction of the ferredoxin-NADP-reductase complex was found to equal to 0.2 M.
M S Khristin, and
E A Akulova, and
V I Surovtsev
December 1978,
European journal of biochemistry,
M S Khristin, and
E A Akulova, and
V I Surovtsev
November 1980,
FEBS letters,
M S Khristin, and
E A Akulova, and
V I Surovtsev
November 1983,
The Journal of biological chemistry,
M S Khristin, and
E A Akulova, and
V I Surovtsev
February 1977,
Biokhimiia (Moscow, Russia),
M S Khristin, and
E A Akulova, and
V I Surovtsev
April 1978,
Biochimica et biophysica acta,
M S Khristin, and
E A Akulova, and
V I Surovtsev
December 1976,
Archives internationales de physiologie et de biochimie,
M S Khristin, and
E A Akulova, and
V I Surovtsev
January 1982,
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie,
M S Khristin, and
E A Akulova, and
V I Surovtsev
December 1965,
Biochemical and biophysical research communications,
M S Khristin, and
E A Akulova, and
V I Surovtsev
January 1986,
Methods in enzymology,
M S Khristin, and
E A Akulova, and
V I Surovtsev
January 1982,
Uspekhi sovremennoi biologii,
