BIOMAT Database Logo BIOMAT Database Logo

[Effect of bcl-2 siRNA on apoptosis of Burkitt's lymphoma cell line CA46]. 2009

Jin-Juan Lin, and Zhen-Xing Lin, and Hao-Bo Huang, and Chen-Qing Zhang, and Lian-Huang Lü
Fujian Province Institute of Hematology, Fujian Medical University, Fuzhou 350001, China.

The aim of this study was to investigate the effect of bcl-2 siRNA on bcl-2 gene expression and apoptosis of lymphoma cell line CA46. A siRNA was designed and synthesized. Then siRNA was transfected into CA46 cells by cationic liposome. At 48 hours after transfection, apoptosis and mitochondria transmembrane potential of CA46 cells were detected by flow cytometry, the expression of bcl-2 mRNA and BCL-2 protein in CA46 cells were detected by RT-PCR and flow cytometry respectively. The results showed that at 48 hours after transfection, apoptosis of CA46 cells occurred, mitochondria transmembrane potential changed. The expression of bcl-2 mRNA and BCL-2 protein in CA46 cells decreased significantly. In conclusion, bcl-2 siRNA depresses the expression of bcl-2 gene in CA46 cells specifically, then changes the mitochondria transmembrane potential, resulting in apoptosis of CA46 cells.

UI MeSH Term Description Entries
D002051 Burkitt Lymphoma A form of undifferentiated malignant LYMPHOMA usually found in central Africa, but also reported in other parts of the world. It is commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. B-cell antigens are expressed on the immature cells that make up the tumor in virtually all cases of Burkitt lymphoma. The Epstein-Barr virus (HERPESVIRUS 4, HUMAN) has been isolated from Burkitt lymphoma cases in Africa and it is implicated as the causative agent in these cases; however, most non-African cases are EBV-negative. African Lymphoma,Burkitt Cell Leukemia,Burkitt Tumor,Lymphoma, Burkitt,Burkitt Leukemia,Burkitt's Leukemia,Burkitt's Lymphoma,Burkitt's Tumor,Leukemia, Lymphoblastic, Burkitt-Type,Leukemia, Lymphocytic, L3,Lymphocytic Leukemia, L3,Burkitts Leukemia,Burkitts Lymphoma,Burkitts Tumor,L3 Lymphocytic Leukemia,L3 Lymphocytic Leukemias,Leukemia, Burkitt,Leukemia, Burkitt Cell,Leukemia, Burkitt's,Leukemia, L3 Lymphocytic,Lymphoma, African,Lymphoma, Burkitt's,Tumor, Burkitt,Tumor, Burkitt's
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D014162 Transfection The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES. Transfections
D017209 Apoptosis A regulated cell death mechanism characterized by distinctive morphologic changes in the nucleus and cytoplasm, including the endonucleolytic cleavage of genomic DNA, at regularly spaced, internucleosomal sites, i.e., DNA FRAGMENTATION. It is genetically programmed and serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth. Apoptosis, Extrinsic Pathway,Apoptosis, Intrinsic Pathway,Caspase-Dependent Apoptosis,Classic Apoptosis,Classical Apoptosis,Programmed Cell Death,Programmed Cell Death, Type I,Apoptoses, Extrinsic Pathway,Apoptoses, Intrinsic Pathway,Apoptosis, Caspase-Dependent,Apoptosis, Classic,Apoptosis, Classical,Caspase Dependent Apoptosis,Cell Death, Programmed,Classic Apoptoses,Extrinsic Pathway Apoptoses,Extrinsic Pathway Apoptosis,Intrinsic Pathway Apoptoses,Intrinsic Pathway Apoptosis
D045744 Cell Line, Tumor A cell line derived from cultured tumor cells. Tumor Cell Line,Cell Lines, Tumor,Line, Tumor Cell,Lines, Tumor Cell,Tumor Cell Lines
D019254 Genes, bcl-2 The B-cell leukemia/lymphoma-2 genes, responsible for blocking apoptosis in normal cells, and associated with follicular lymphoma when overexpressed. Overexpression results from the t(14;18) translocation. The human c-bcl-2 gene is located at 18q24 on the long arm of chromosome 18. bcl-2 Genes,c-bcl-2 Genes,c-bcl-2 Proto-Oncogenes,Gene, bcl-2,Gene, c-bcl-2,Genes, bcl 2,Genes, c-bcl-2,Proto-Oncogene, c-bcl-2,Proto-Oncogenes, c-bcl-2,bcl 2 Genes,bcl-2 Gene,c bcl 2 Genes,c bcl 2 Proto Oncogenes,c-bcl-2 Gene,c-bcl-2 Proto-Oncogene
D020133 Reverse Transcriptase Polymerase Chain Reaction A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols. Polymerase Chain Reaction, Reverse Transcriptase,Reverse Transcriptase PCR,PCR, Reverse Transcriptase,Transcriptase PCR, Reverse
D034741 RNA, Small Interfering Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions. RNA, Scan,Repeat-Associated siRNA,Scan RNA,Small Scan RNA,Trans-Acting siRNA,siRNA,siRNA, Repeat-Associated,siRNA, Trans-Acting,Short Hairpin RNA,Short Interfering RNA,Small Hairpin RNA,Small Interfering RNA,scnRNA,shRNA,tasiRNA,Hairpin RNA, Short,Hairpin RNA, Small,Interfering RNA, Short,Interfering RNA, Small,RNA, Short Hairpin,RNA, Short Interfering,RNA, Small Hairpin,RNA, Small Scan,Repeat Associated siRNA,Scan RNA, Small,Trans Acting siRNA,siRNA, Repeat Associated,siRNA, Trans Acting

Related Publications

Jin-Juan Lin, and Zhen-Xing Lin, and Hao-Bo Huang, and Chen-Qing Zhang, and Lian-Huang Lü
[Effect of bortezomib on lymphoma cell line CA46 and its relative mechanisms].
August 2010, Zhongguo shi yan xue ye xue za zhi,
Jin-Juan Lin, and Zhen-Xing Lin, and Hao-Bo Huang, and Chen-Qing Zhang, and Lian-Huang Lü
Antiproliferative effect on interferon on a Burkitt's lymphoma cell line.
December 1980, Experimental cell research,
Jin-Juan Lin, and Zhen-Xing Lin, and Hao-Bo Huang, and Chen-Qing Zhang, and Lian-Huang Lü
[Apoptosis of Burkitt's lymphoma Raji cell line induced by bortezomib].
June 2009, Zhongguo shi yan xue ye xue za zhi,
Jin-Juan Lin, and Zhen-Xing Lin, and Hao-Bo Huang, and Chen-Qing Zhang, and Lian-Huang Lü
Role of bcl-2 in Epstein-Barr virus-induced malignant conversion of Burkitt's lymphoma cell line Akata.
February 2001, Journal of virology,
Jin-Juan Lin, and Zhen-Xing Lin, and Hao-Bo Huang, and Chen-Qing Zhang, and Lian-Huang Lü
Resistance to etoposide-induced apoptosis in a Burkitt's lymphoma cell line.
August 1998, International journal of cancer,
Jin-Juan Lin, and Zhen-Xing Lin, and Hao-Bo Huang, and Chen-Qing Zhang, and Lian-Huang Lü
Etodolac inhibits EBER expression and induces Bcl-2-regulated apoptosis in Burkitt's lymphoma cells.
September 2005, European journal of haematology,
Jin-Juan Lin, and Zhen-Xing Lin, and Hao-Bo Huang, and Chen-Qing Zhang, and Lian-Huang Lü
STAT3 knockdown by siRNA induces apoptosis in human cutaneous T-cell lymphoma line Hut78 via downregulation of Bcl-xL.
June 2010, Cellular & molecular biology letters,
Jin-Juan Lin, and Zhen-Xing Lin, and Hao-Bo Huang, and Chen-Qing Zhang, and Lian-Huang Lü
[Fas mediated apoptosis inhibited by human bcl-2 gene in lymphoma cell line Jurkat].
May 1998, Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi,
Jin-Juan Lin, and Zhen-Xing Lin, and Hao-Bo Huang, and Chen-Qing Zhang, and Lian-Huang Lü
The Effect of Resveratrol on Cell Viability in the Burkitt's Lymphoma Cell Line Ramos.
December 2017, Molecules (Basel, Switzerland),
Jin-Juan Lin, and Zhen-Xing Lin, and Hao-Bo Huang, and Chen-Qing Zhang, and Lian-Huang Lü
Small interfering RNA (siRNA) against the Bcl-2 gene increases apoptosis in a canine melanoma cell line.
March 2010, The Journal of veterinary medical science,
Copied contents to your clipboard!