BIOMAT Database Logo BIOMAT Database Logo

Biochemical and structural characterization of 5'-methylthioadenosine nucleosidases from Arabidopsis thaliana. 2009

Eun Young Park, and Woo Suk Choi, and Seung-Ick Oh, and Kyung-Nam Kim, and Jeong Sheop Shin, and Hyun Kyu Song
Korea University, Anam-Dong, Seongbuk-Gu, Seoul, Republic of Korea.

5'-Methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH) are important metabolites in all living organisms. Two similar nucleosidases for hydrolyzing MTA in Arabidopsis thaliana (AtMTAN1 and AtMTAN2) exist, but only AtMTAN2 shows markedly broad substrate specificity for hydrolysis of SAH. To examine the biochemical characteristics of AtMTAN2, it was over-expressed in Escherichia coli and purified to homogeneity. Spectroscopic assays confirm AtMTAN2 catalyzes MTA as well as SAH hydrolysis, compared to AtMTAN1 which only hydrolyzes MTA. In addition, crystal structure of the AtMTAN2 enzyme in complex with, adenine was determined at 2.9A resolution. Finally, a structural comparison of AtMTAN2 performed with previously determined structures of AtMTAN1 and an E. coli homolog provides clues for the substrate specificity of MTA nucleosidases in A. thaliana.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D011487 Protein Conformation The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain). Conformation, Protein,Conformations, Protein,Protein Conformations
D011683 Purine-Nucleoside Phosphorylase An enzyme that catalyzes the reaction between a purine nucleoside and orthophosphate to form a free purine plus ribose-5-phosphate. EC 2.4.2.1. Inosine Phosphorylase,Nicotinamide Riboside Phosphorylase,Purine Nucleoside Phosphorylases,Nucleoside Phosphorylases, Purine,Phosphorylase, Inosine,Phosphorylase, Nicotinamide Riboside,Phosphorylase, Purine-Nucleoside,Phosphorylases, Purine Nucleoside,Purine Nucleoside Phosphorylase,Riboside Phosphorylase, Nicotinamide
D002384 Catalysis The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction. Catalyses
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D017360 Arabidopsis A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development. Arabidopsis thaliana,Cress, Mouse-ear,A. thaliana,A. thalianas,Arabidopses,Arabidopsis thalianas,Cress, Mouse ear,Cresses, Mouse-ear,Mouse-ear Cress,Mouse-ear Cresses,thaliana, A.,thaliana, Arabidopsis,thalianas, A.
D018360 Crystallography, X-Ray The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) X-Ray Crystallography,Crystallography, X Ray,Crystallography, Xray,X Ray Crystallography,Xray Crystallography,Crystallographies, X Ray,X Ray Crystallographies
D029681 Arabidopsis Proteins Proteins that originate from plants species belonging to the genus ARABIDOPSIS. The most intensely studied species of Arabidopsis, Arabidopsis thaliana, is commonly used in laboratory experiments. Arabidopsis thaliana Proteins,Thale Cress Proteins,Proteins, Arabidopsis thaliana,thaliana Proteins, Arabidopsis

Related Publications

Eun Young Park, and Woo Suk Choi, and Seung-Ick Oh, and Kyung-Nam Kim, and Jeong Sheop Shin, and Hyun Kyu Song
Structural and biochemical characterization of mitochondrial citrate synthase 4 from Arabidopsis thaliana.
March 2020, Acta crystallographica. Section F, Structural biology communications,
Eun Young Park, and Woo Suk Choi, and Seung-Ick Oh, and Kyung-Nam Kim, and Jeong Sheop Shin, and Hyun Kyu Song
Overexpression, purification, biochemical and structural characterization of rhamnosyltransferase UGT89C1 from Arabidopsis thaliana.
April 2019, Protein expression and purification,
Eun Young Park, and Woo Suk Choi, and Seung-Ick Oh, and Kyung-Nam Kim, and Jeong Sheop Shin, and Hyun Kyu Song
Characterization of 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidases from Borrelia burgdorferi: Antibiotic targets for Lyme disease.
January 2020, Biochimica et biophysica acta. General subjects,
Eun Young Park, and Woo Suk Choi, and Seung-Ick Oh, and Kyung-Nam Kim, and Jeong Sheop Shin, and Hyun Kyu Song
Crystal structure of 5'-methylthioadenosine nucleosidase from Arabidopsis thaliana at 1.5-A resolution.
November 2006, Proteins,
Eun Young Park, and Woo Suk Choi, and Seung-Ick Oh, and Kyung-Nam Kim, and Jeong Sheop Shin, and Hyun Kyu Song
Biochemical characterization of phosphoenolpyruvate carboxykinases from Arabidopsis thaliana.
October 2019, The Biochemical journal,
Eun Young Park, and Woo Suk Choi, and Seung-Ick Oh, and Kyung-Nam Kim, and Jeong Sheop Shin, and Hyun Kyu Song
Molecular determinants of substrate specificity in plant 5'-methylthioadenosine nucleosidases.
April 2008, Journal of molecular biology,
Eun Young Park, and Woo Suk Choi, and Seung-Ick Oh, and Kyung-Nam Kim, and Jeong Sheop Shin, and Hyun Kyu Song
Mechanism of substrate specificity in 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidases.
January 2011, Journal of structural biology,
Eun Young Park, and Woo Suk Choi, and Seung-Ick Oh, and Kyung-Nam Kim, and Jeong Sheop Shin, and Hyun Kyu Song
Picomolar inhibitors as transition-state probes of 5'-methylthioadenosine nucleosidases.
November 2007, ACS chemical biology,
Eun Young Park, and Woo Suk Choi, and Seung-Ick Oh, and Kyung-Nam Kim, and Jeong Sheop Shin, and Hyun Kyu Song
Femtomolar inhibitors bind to 5'-methylthioadenosine nucleosidases with favorable enthalpy and entropy.
September 2012, Biochemistry,
Eun Young Park, and Woo Suk Choi, and Seung-Ick Oh, and Kyung-Nam Kim, and Jeong Sheop Shin, and Hyun Kyu Song
Biochemical and structural properties of cyanases from Arabidopsis thaliana and Oryza sativa.
March 2011, PloS one,
Copied contents to your clipboard!