The present study evaluated the effects of food and alcohol intake on the nutritional and metabolic status of male and female periadolescent rats submitted to single (15%) and multiple (10%, 20%, 30%) concentrations of hydroalcoholic solutions of sugar-based alcohol associated with a feed mixture. Thirty-six periadolescent Wistar rats were used and randomly arranged into three groups: Group A (control; 0% ethanol; six males and six females), Group B (15% ethanol; six males and six females), and Group C (10%, 20%, and 30% ethanol; six males and six females). Food consumption, body weight, water intake (mL), ethanol intake (g/kg/day), ethanol preference in relation to water and different concentrations, and serum biochemical dosages (glucose, total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein (HDL) cholesterol, very low-density lipoprotein fraction, triglycerides, cholesterol/HDL [CT/HDL], albumin) were analyzed. Males from Group C ingested more feed than females, which consumed reducing amounts throughout the weeks studied. Males also had heavier body weight, which increased throughout the experimental period. The animals ingested more water (females ingested more than males) in the first experimental week. Group C had a higher ethanol intake and greater preference for ethanol over water in both genders than Group B, which decreased over the subsequent weeks. Serum glucose was lower in Group A, whereas the CT/HDL ratio was lower in Group C. These findings allow the conclusion that nutritional and metabolic impact resulting from alcohol intake is different between genders and between the different forms in which the drug is offered. It is important to warn the population about the concentrations of alcohol intake, which may influence the growth and development of adolescents, thereby compromising their quality of life.