Differential interference contrast microscope using photonic crystals for phase imaging and three-dimensional shape reconstruction. 2009

Laurent Fabre, and Yoshihiko Inoue, and Takafumi Aoki, and Shojiro Kawakami
Graduate School of Information Sciences, Tohoku University, 6-6-04 Aramaki Aza Aoba, Aoba-ku,Sendai, Miyagi 980-8579, Japan. lfabre@upstream-lab.com

We present a differential interference contrast microscope using photonic crystals capable of real-time capture of both phase and amplitude components independently without moving parts. Unlike previous methods using rotating polarizers to discriminate each component, we propose using a special camera equipped with an arrayed polarizer whose instant polarization measurement allows real-time acquisition of the phase gradient information. A two-image algorithm is used to reconstruct the phase two-dimensional distribution of biological samples from the gradient information with a transmission-type microscope. We also talk about deducing a sample's three-dimensional shape for a reflection-type microscope. The efficiency of the method is demonstrated experimentally.

UI MeSH Term Description Entries
D007091 Image Processing, Computer-Assisted A technique of inputting two-dimensional or three-dimensional images into a computer and then enhancing or analyzing the imagery into a form that is more useful to the human observer. Biomedical Image Processing,Computer-Assisted Image Processing,Digital Image Processing,Image Analysis, Computer-Assisted,Image Reconstruction,Medical Image Processing,Analysis, Computer-Assisted Image,Computer-Assisted Image Analysis,Computer Assisted Image Analysis,Computer Assisted Image Processing,Computer-Assisted Image Analyses,Image Analyses, Computer-Assisted,Image Analysis, Computer Assisted,Image Processing, Biomedical,Image Processing, Computer Assisted,Image Processing, Digital,Image Processing, Medical,Image Processings, Medical,Image Reconstructions,Medical Image Processings,Processing, Biomedical Image,Processing, Digital Image,Processing, Medical Image,Processings, Digital Image,Processings, Medical Image,Reconstruction, Image,Reconstructions, Image
D008857 Microscopy, Interference The science and application of a double-beam transmission interference microscope in which the illuminating light beam is split into two paths. One beam passes through the specimen while the other beam reflects off a reference mirror before joining and interfering with the other. The observed optical path difference between the two beams can be measured and used to discriminate minute differences in thickness and refraction of non-stained transparent specimens, such as living cells in culture. Interferometry, Microscopic,Microinterferometry,Microscopy, Differential Interference Contrast,Microscopy, Interference Reflection,Microscopy, Nomarski Interference Contrast,Interference Microscopy,Interference Reflection Microscopy,Microscopic Interferometry,Reflection Microscopy, Interference
D010781 Photography Method of making images on a sensitized surface by exposure to light or other radiant energy. Photographies
D003199 Computer Systems Systems composed of a computer or computers, peripheral equipment, such as disks, printers, and terminals, and telecommunications capabilities. Computer Architecture,Computer System,Computer Systems Development,Computer Systems Evaluation,Computer Systems Organization,Real Time System,Real-Time Systems,Organization, Computer Systems,Architecture, Computer,Architectures, Computer,Computer Architectures,Computer Systems Evaluations,Development, Computer Systems,Evaluation, Computer Systems,Evaluations, Computer Systems,Real Time Systems,Real-Time System,System, Computer,System, Real Time,System, Real-Time,Systems, Computer,Systems, Real Time,Systems, Real-Time,Time System, Real,Time Systems, Real
D006367 HeLa Cells The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for, among other things, VIRUS CULTIVATION and PRECLINICAL DRUG EVALUATION assays. Cell, HeLa,Cells, HeLa,HeLa Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000465 Algorithms A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task. Algorithm
D055095 Optics and Photonics A specialized field of physics and engineering involved in studying the behavior and properties of light and the technology of analyzing, generating, transmitting, and manipulating ELECTROMAGNETIC RADIATION in the visible, infrared, and ultraviolet range. Photonics,Photonics and Optics
D021621 Imaging, Three-Dimensional The process of generating three-dimensional images by electronic, photographic, or other methods. For example, three-dimensional images can be generated by assembling multiple tomographic images with the aid of a computer, while photographic 3-D images (HOLOGRAPHY) can be made by exposing film to the interference pattern created when two laser light sources shine on an object. Computer-Assisted Three-Dimensional Imaging,Imaging, Three-Dimensional, Computer Assisted,3-D Image,3-D Imaging,Computer-Generated 3D Imaging,Three-Dimensional Image,Three-Dimensional Imaging, Computer Generated,3 D Image,3 D Imaging,3-D Images,3-D Imagings,3D Imaging, Computer-Generated,3D Imagings, Computer-Generated,Computer Assisted Three Dimensional Imaging,Computer Generated 3D Imaging,Computer-Assisted Three-Dimensional Imagings,Computer-Generated 3D Imagings,Image, 3-D,Image, Three-Dimensional,Images, 3-D,Images, Three-Dimensional,Imaging, 3-D,Imaging, Computer-Assisted Three-Dimensional,Imaging, Computer-Generated 3D,Imaging, Three Dimensional,Imagings, 3-D,Imagings, Computer-Assisted Three-Dimensional,Imagings, Computer-Generated 3D,Imagings, Three-Dimensional,Three Dimensional Image,Three Dimensional Imaging, Computer Generated,Three-Dimensional Images,Three-Dimensional Imaging,Three-Dimensional Imaging, Computer-Assisted,Three-Dimensional Imagings,Three-Dimensional Imagings, Computer-Assisted

Related Publications

Laurent Fabre, and Yoshihiko Inoue, and Takafumi Aoki, and Shojiro Kawakami
February 2012, Journal of biomedical optics,
Laurent Fabre, and Yoshihiko Inoue, and Takafumi Aoki, and Shojiro Kawakami
April 2004, Journal of microscopy,
Laurent Fabre, and Yoshihiko Inoue, and Takafumi Aoki, and Shojiro Kawakami
April 1995, Journal of microscopy,
Laurent Fabre, and Yoshihiko Inoue, and Takafumi Aoki, and Shojiro Kawakami
September 1990, Applied optics,
Laurent Fabre, and Yoshihiko Inoue, and Takafumi Aoki, and Shojiro Kawakami
February 1992, Applied optics,
Laurent Fabre, and Yoshihiko Inoue, and Takafumi Aoki, and Shojiro Kawakami
May 2002, Optics letters,
Laurent Fabre, and Yoshihiko Inoue, and Takafumi Aoki, and Shojiro Kawakami
June 2006, Physical review letters,
Laurent Fabre, and Yoshihiko Inoue, and Takafumi Aoki, and Shojiro Kawakami
May 1996, Applied optics,
Laurent Fabre, and Yoshihiko Inoue, and Takafumi Aoki, and Shojiro Kawakami
August 2018, Scientific reports,
Laurent Fabre, and Yoshihiko Inoue, and Takafumi Aoki, and Shojiro Kawakami
November 2008, Journal of microscopy,
Copied contents to your clipboard!