[Determination of melamine residue in raw milk and dairy products using hydrophilic interaction chromatography-electrospray ionization tandem mass spectrometry]. 2008

Lijuan Yan, and Min Wu, and Zhigang Zhang, and Yu Zhou, and Liyi Lin, and Enhua Fang, and Dunming Xu, and Luping Chen
Xiamen Entry-Exit Inspection and Quarantine Bureau, Xiamen 361012, China. yanlj@xmciq.gov.cn

A method for the determination of melamine residue in raw milk and dairy products was developed using hydrophilic interaction chromatography-electrospray ionization tandem mass spectrometry (HILIC-ESI-MS/MS). The melamine residue in the test sample was extracted with 1% trichloroacetic acid-acetonitrile (1 : 1, v/v) solution. The homogenate was centrifuged and the supernatant was collected. The extract was cleaned up using a mixed-mode cation exchange (MCX) solid-phase extraction cartridge and then concentrated, and analyzed by HILIC-ESI-MS/MS. The gradient chromatographic separation was performed using a hydrophilic silica column (250 mm x 4.6 mm, 5 microm) with 10 mmol/L ammonium acetate buffer (pH 3.0) and acetonitrile as the mobile phases. Due to its hydrophilic property, melamine was well retained on the column and seperated from other compounds. It effectively reduced matrix effect. A triple quadruple mass spectrometer equipped with an electrospray ionization source was employed for the qualitative and quantitative measurement of melamine. The melamine was quantified using the fragments produced from the protonated melamine ion through multiple reaction monitoring (MRM) in positive ion mode. Two MRM transitions from the protonated melamine ion (m/z 127 --> 85 and m/z 127 --> 68) were monitored. The average recoveries were between 76.3% and 98.7% in the spiked range of 0.5 to 10 mg/kg in raw milk and dairy products, and the relative standard deviations were less than 6.8%. The linear range was from 0.05 to 10.0 mg/L. The limit of quantification (S/N > 10) was 0.05 mg/kg. The method is highly selective and sensitive for the measurements of melamine and adequate for the analysis of melamine in raw milk and dairy products.

UI MeSH Term Description Entries
D008892 Milk The off-white liquid secreted by the mammary glands of humans and other mammals. It contains proteins, sugar, lipids, vitamins, and minerals. Cow Milk,Cow's Milk,Milk, Cow,Milk, Cow's
D011786 Quality Control A system for verifying and maintaining a desired level of quality in a product or process by careful planning, use of proper equipment, continued inspection, and corrective action as required. (Random House Unabridged Dictionary, 2d ed) Control, Quality,Controls, Quality,Quality Controls
D005504 Food Analysis Measurement and evaluation of the components of substances to be taken as FOOD. Analysis, Food,Analyses, Food,Food Analyses
D005506 Food Contamination The presence in food of harmful, unpalatable, or otherwise objectionable foreign substances, e.g. chemicals, microorganisms or diluents, before, during, or after processing or storage. Food Adulteration,Adulteration, Food,Adulterations, Food,Contamination, Food,Contaminations, Food,Food Adulterations,Food Contaminations
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D014227 Triazines Heterocyclic rings containing three nitrogen atoms, commonly in 1,2,4 or 1,3,5 or 2,4,6 formats. Some are used as HERBICIDES. Triazine,Benzotriazines
D016014 Linear Models Statistical models in which the value of a parameter for a given value of a factor is assumed to be equal to a + bx, where a and b are constants. The models predict a linear regression. Linear Regression,Log-Linear Models,Models, Linear,Linear Model,Linear Regressions,Log Linear Models,Log-Linear Model,Model, Linear,Model, Log-Linear,Models, Log-Linear,Regression, Linear,Regressions, Linear
D053719 Tandem Mass Spectrometry A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection. Mass Spectrometry-Mass Spectrometry,Mass Spectrometry Mass Spectrometry,Mass Spectrometry, Tandem
D057927 Hydrophobic and Hydrophilic Interactions The thermodynamic interaction between a substance and WATER. Hydrophilic Interactions,Hydrophilic and Hydrophobic Interactions,Hydrophilicity,Hydrophobic Interactions,Hydrophobicity,Hydrophilic Interaction,Hydrophilicities,Hydrophobic Interaction,Hydrophobicities,Interaction, Hydrophilic,Interaction, Hydrophobic,Interactions, Hydrophilic,Interactions, Hydrophobic
D021241 Spectrometry, Mass, Electrospray Ionization A mass spectrometry technique used for analysis of nonvolatile compounds such as proteins and macromolecules. The technique involves preparing electrically charged droplets from analyte molecules dissolved in solvent. The electrically charged droplets enter a vacuum chamber where the solvent is evaporated. Evaporation of solvent reduces the droplet size, thereby increasing the coulombic repulsion within the droplet. As the charged droplets get smaller, the excess charge within them causes them to disintegrate and release analyte molecules. The volatilized analyte molecules are then analyzed by mass spectrometry. ESI Mass Spectrometry,Electrospray Ionization Mass Spectrometry,Mass Spectrometry, ESI,Spectrometry, ESI Mass

Related Publications

Lijuan Yan, and Min Wu, and Zhigang Zhang, and Yu Zhou, and Liyi Lin, and Enhua Fang, and Dunming Xu, and Luping Chen
April 2006, Journal of agricultural and food chemistry,
Lijuan Yan, and Min Wu, and Zhigang Zhang, and Yu Zhou, and Liyi Lin, and Enhua Fang, and Dunming Xu, and Luping Chen
December 2010, Se pu = Chinese journal of chromatography,
Lijuan Yan, and Min Wu, and Zhigang Zhang, and Yu Zhou, and Liyi Lin, and Enhua Fang, and Dunming Xu, and Luping Chen
September 2015, Se pu = Chinese journal of chromatography,
Lijuan Yan, and Min Wu, and Zhigang Zhang, and Yu Zhou, and Liyi Lin, and Enhua Fang, and Dunming Xu, and Luping Chen
November 2008, Se pu = Chinese journal of chromatography,
Lijuan Yan, and Min Wu, and Zhigang Zhang, and Yu Zhou, and Liyi Lin, and Enhua Fang, and Dunming Xu, and Luping Chen
July 2014, Journal of separation science,
Lijuan Yan, and Min Wu, and Zhigang Zhang, and Yu Zhou, and Liyi Lin, and Enhua Fang, and Dunming Xu, and Luping Chen
September 2010, Journal of separation science,
Lijuan Yan, and Min Wu, and Zhigang Zhang, and Yu Zhou, and Liyi Lin, and Enhua Fang, and Dunming Xu, and Luping Chen
March 2008, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
Lijuan Yan, and Min Wu, and Zhigang Zhang, and Yu Zhou, and Liyi Lin, and Enhua Fang, and Dunming Xu, and Luping Chen
June 2015, Journal of separation science,
Lijuan Yan, and Min Wu, and Zhigang Zhang, and Yu Zhou, and Liyi Lin, and Enhua Fang, and Dunming Xu, and Luping Chen
June 2010, Journal of analytical toxicology,
Lijuan Yan, and Min Wu, and Zhigang Zhang, and Yu Zhou, and Liyi Lin, and Enhua Fang, and Dunming Xu, and Luping Chen
May 2008, Se pu = Chinese journal of chromatography,
Copied contents to your clipboard!