BIOMAT Database Logo BIOMAT Database Logo

Reduced antigenicity of naturally occurring hepatitis B surface antigen variants with substitutions at the amino acid residue 126. 2008

Song Qiu, and Jiming Zhang, and Yongjun Tian, and Yinke Yang, and Hanju Huang, and Dongliang Yang, and Mengji Lu, and Yang Xu
Department of Microbiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.

BACKGROUND Substitutions at amino acid residue 126 of hepatitis B surface antigen (HBsAg) occur frequently in hepatitis B virus (HBV) isolates from patients with chronic HBV infection. These substitutions occur naturally, but their significance for viral persistence is unclear and requires further investigation. METHODS We amplified coding regions of HBsAg by PCR using sera from 1 patient chronically infected with HBV. Three representative clones of HBsAg with amino acid residues 126Ile (I), 126Thr (T) and 126Ser (S) were selected from sequenced clones. HBsAg 126Ala (A) mutants of subtype C/adr and D/adw were generated by site-directed mutagenesis. The HBsAg expression vectors were constructed and transiently transfected into HepG2 cells. The binding reactivity of HBsAg to anti-HBs antibodies was tested by chemiluminescent microparticle immunoassay and by immunofluorescence staining with polyclonal and monoclonal anti-HBs antibodies. RESULTS Diverse HBsAg variants with substitutions at amino acid residue 126 co-existed in a chronically infected HBV patient. HBsAg with the substitution 126S showed a significantly low antigenicity, while the binding reactivity to anti-HBs of other HBsAg with 126I, 126T and 126A were comparable. CONCLUSIONS HBsAg with the 126S substitution has a reduced antigenicity, which may contribute to immune escape in chronic HBV infection.

UI MeSH Term Description Entries
D008297 Male Males
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D004279 DNA, Viral Deoxyribonucleic acid that makes up the genetic material of viruses. Viral DNA
D005838 Genotype The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS. Genogroup,Genogroups,Genotypes
D006509 Hepatitis B INFLAMMATION of the LIVER in humans caused by a member of the ORTHOHEPADNAVIRUS genus, HEPATITIS B VIRUS. It is primarily transmitted by parenteral exposure, such as transfusion of contaminated blood or blood products, but can also be transmitted via sexual or intimate personal contact. Hepatitis B Virus Infection
D006514 Hepatitis B Surface Antigens Those hepatitis B antigens found on the surface of the Dane particle and on the 20 nm spherical and tubular particles. Several subspecificities of the surface antigen are known. These were formerly called the Australia antigen. Australia Antigen,HBsAg,Hepatitis B Surface Antigen,Antigen, Australia
D006515 Hepatitis B virus The type species of the genus ORTHOHEPADNAVIRUS which causes human HEPATITIS B and is also apparently a causal agent in human HEPATOCELLULAR CARCINOMA. The Dane particle is an intact hepatitis virion, named after its discoverer. Non-infectious spherical and tubular particles are also seen in the serum. Dane Particle,Hepatitis Virus, Homologous Serum,B virus, Hepatitis,Hepatitis B viruses,Particle, Dane,viruses, Hepatitis B
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein

Related Publications

Song Qiu, and Jiming Zhang, and Yongjun Tian, and Yinke Yang, and Hanju Huang, and Dongliang Yang, and Mengji Lu, and Yang Xu
Naturally Occurring Surface Antigen Variants of Hepatitis B Virus in Tunisian Patients.
January 2016, Intervirology,
Song Qiu, and Jiming Zhang, and Yongjun Tian, and Yinke Yang, and Hanju Huang, and Dongliang Yang, and Mengji Lu, and Yang Xu
Virion Secretion of Hepatitis B Virus Naturally Occurring Core Antigen Variants.
December 2020, Cells,
Song Qiu, and Jiming Zhang, and Yongjun Tian, and Yinke Yang, and Hanju Huang, and Dongliang Yang, and Mengji Lu, and Yang Xu
Biological significance of amino acid substitutions in hepatitis B surface antigen (HBsAg) for glycosylation, secretion, antigenicity and immunogenicity of HBsAg and hepatitis B virus replication.
February 2010, The Journal of general virology,
Song Qiu, and Jiming Zhang, and Yongjun Tian, and Yinke Yang, and Hanju Huang, and Dongliang Yang, and Mengji Lu, and Yang Xu
Naturally occurring hepatitis B virus surface antigen mutant variants in Malaysian blood donors and vaccinees.
July 2015, European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology,
Song Qiu, and Jiming Zhang, and Yongjun Tian, and Yinke Yang, and Hanju Huang, and Dongliang Yang, and Mengji Lu, and Yang Xu
Amino acid substitutions in naturally occurring variants of ail result in altered invasion activity.
February 1992, Journal of bacteriology,
Song Qiu, and Jiming Zhang, and Yongjun Tian, and Yinke Yang, and Hanju Huang, and Dongliang Yang, and Mengji Lu, and Yang Xu
Naturally occurring variants of hepatitis B virus.
January 1999, Advances in virus research,
Song Qiu, and Jiming Zhang, and Yongjun Tian, and Yinke Yang, and Hanju Huang, and Dongliang Yang, and Mengji Lu, and Yang Xu
Prevalence of naturally occurring surface antigen variants of hepatitis B virus in Korean patients infected chronically.
June 2005, Journal of medical virology,
Song Qiu, and Jiming Zhang, and Yongjun Tian, and Yinke Yang, and Hanju Huang, and Dongliang Yang, and Mengji Lu, and Yang Xu
Prevalence of naturally occurring surface gene variants of hepatitis B virus in nonimmunized surface antigen-negative Chinese carriers.
November 2001, Hepatology (Baltimore, Md.),
Song Qiu, and Jiming Zhang, and Yongjun Tian, and Yinke Yang, and Hanju Huang, and Dongliang Yang, and Mengji Lu, and Yang Xu
The amino Acid residues at positions 120 to 123 are crucial for the antigenicity of hepatitis B surface antigen.
September 2007, Journal of clinical microbiology,
Song Qiu, and Jiming Zhang, and Yongjun Tian, and Yinke Yang, and Hanju Huang, and Dongliang Yang, and Mengji Lu, and Yang Xu
Naturally occurring amino-acid substitutions to nucleos(t)ide analogues in treatment naive Turkish patients with chronic hepatitis B.
January 2010, Journal of viral hepatitis,
Copied contents to your clipboard!