Biomaterial Database

[Effect of additives on accumulation of glycyrrhizin in suspension culture cells of Glycyrrhiza uralensis]. 2008

Ai-hua Bian, and Wen-yuan Gao, and Juan Wang

School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.

OBJECTIVE To investigate the effects of several additives (sodium acetate, phenylalanine and amidocaproic acid) on glycyrrhizin production in Glycyrrhiza uralensis cells. METHODS The different concentration of additives were administrated into the medium at the beginning of the culture. The glycyrrhizin content in G. uralensis cells was analyzed by HPLC. RESULTS The lower sodium acetate concentration of 0.1 mmol x L(-1) enhanced the glycyrrhizin content by 2.4 times and the higher sodium acetate concentration resulted in the higher accumulation of cell biomass. Glycyrrhizin content increased slightly when the phenylalanine dosages increased gradually from 0.1-2 mmol x L(-1). The highest glycyrrhizin content of 14.10 microg x g(-1) was obtained with the addition of 2 mmol x L(-1) phenylalanine which was 3.60 times compared with the control. The addition of 0.1 mmol x L(-1) amidocaproic acid increased the glycyrrhizin content by 2. 24 times. With the increase of the concentration of amidocaproic acid, the glycyrrhizin content decreased slightly and the higher concentration of 2 mol x L(-1) inhibited the accumulation of glycyrrhizin. CONCLUSIONS The addition of sodium acetate, phenylalanine and amidocaproic acid to the medium were effective approaches to enhance the glycyrrhizin content in G. uralensis cells.

UI	MeSH Term	Description	Entries
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D018929	Cell Culture Techniques	Methods for maintaining or growing CELLS in vitro.	Cell Culture,Cell Culture Technique,Cell Cultures,Culture Technique, Cell,Culture Techniques, Cell
D019695	Glycyrrhizic Acid	A widely used anti-inflammatory agent isolated from the licorice root. It is metabolized to GLYCYRRHETINIC ACID, which inhibits 11-BETA-HYDROXYSTEROID DEHYDROGENASES and other enzymes involved in the metabolism of CORTICOSTEROIDS. Therefore, glycyrrhizic acid, which is the main and sweet component of licorice, has been investigated for its ability to cause hypermineralocorticoidism with sodium retention and potassium loss, edema, increased blood pressure, as well as depression of the renin-angiotensin-aldosterone system.	Glycyrrhizin,Diammonium Glycyrrhizinate,Dipotassium Glycyrrhizinate,Glycyrrhizinic Acid,Zinc Glycyrrhizate,Acid, Glycyrrhizic,Acid, Glycyrrhizinic,Glycyrrhizate, Zinc,Glycyrrhizinate, Diammonium,Glycyrrhizinate, Dipotassium
D029882	Glycyrrhiza uralensis	A plant species of the family FABACEAE.	Chinese Licorice,Gan cao,Gan zao,Chinese Licorices,Gan caos,Gan zaos,Glycyrrhiza uralenses,Licorice, Chinese,Licorices, Chinese,cao, Gan,uralenses, Glycyrrhiza,zaos, Gan