BIOMAT Database Logo BIOMAT Database Logo

Molecular identification of ovine Theileria species by a new PCR-RFLP method. 2009

M Heidarpour Bami, and H R Haddadzadeh, and B Kazemi, and P Khazraiinia, and M Bandehpour, and M Aktas
Clinical Science Department, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

Theileria spp. infect wild and domestic ruminants in the tropical and subtropical regions of the world. Two species, T. lestoquardi and T. ovis, are suspected to cause ovine theileriosis in Iran. The epidemiological aspects of ovine theileriosis in Iran are poorly understood, and further investigations by sensitive and precise techniques are required. In this study, the use of a nested PCR for amplification of a fragment of the 18S ribosomal DNA from virtually all species of Theileria is described. For differentiation of various Theileria spp. a RFLP assay was developed as a diagnostic tool enabling direct, concurrent, highly specific and sensitive identification of Theileria spp. The sensitivity of the nested PCR for Theileria species was 10(-5)% parasitemia. Restriction fragment length polymorphism (RFLP) of the PCR products allowed differentiation between three different Theileria species (T. annulata, T. lestoquardi and T. ovis) and seems to be useful for differentiation of other species such as T. separata and Theileria spp. china. From 100 field blood samples obtained from sheep in East and South-East Iran, 56% were positive for Theileria spp. by nested-PCR compared with 21% by microscopic examination. Out of 56 positive samples, 12.5% (7/56) were positive for T. ovis and 87/5% (49/56) were positive for T. lestoquardi. This is the first report in which T. ovis has been detected in Iran using molecular identification techniques.

UI MeSH Term Description Entries
D007492 Iran A country bordering the Gulf of Oman, the Persian Gulf, and the Caspian Sea, between Iraq and Pakistan. The capital is Tehran. Islamic Republic of Iran
D012150 Polymorphism, Restriction Fragment Length Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment. RFLP,Restriction Fragment Length Polymorphism,RFLPs,Restriction Fragment Length Polymorphisms
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D012756 Sheep Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS. Ovis,Sheep, Dall,Dall Sheep,Ovis dalli
D012757 Sheep Diseases Diseases of domestic and mountain sheep of the genus Ovis. Ovine Diseases,Disease, Ovine,Disease, Sheep,Diseases, Ovine,Diseases, Sheep,Ovine Disease,Sheep Disease
D013801 Theileriasis Infection of cattle, sheep, or goats with protozoa of the genus THEILERIA. This infection results in an acute or chronic febrile condition. Corridor Disease,East Coast Fever,Theileriosis,Coast Fever, East,Disease, Corridor,Fever, East Coast,Theileriases,Theilerioses
D016133 Polymerase Chain Reaction In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain
D016794 Theileria A genus of tick-borne protozoa parasitic in the lymphocytes, erythrocytes, and endothelial cells of mammals. Its organisms multiply asexually and then invade erythrocytes, where they undergo no further reproduction until ingested by a transmitting tick. Theilerias

Related Publications

M Heidarpour Bami, and H R Haddadzadeh, and B Kazemi, and P Khazraiinia, and M Bandehpour, and M Aktas
Molecular Detection and Identification of Theileria Species by PCR-RFLP Method in Sheep from Ahvaz, Southern Iran.
March 2014, Iranian journal of parasitology,
M Heidarpour Bami, and H R Haddadzadeh, and B Kazemi, and P Khazraiinia, and M Bandehpour, and M Aktas
Molecular identification of Theileria species in naturally infected sheep using nested PCR-RFLP.
May 2022, Parasitology research,
M Heidarpour Bami, and H R Haddadzadeh, and B Kazemi, and P Khazraiinia, and M Bandehpour, and M Aktas
Molecular identification of Indian crocodile species: PCR-RFLP method for forensic authentication*.
September 2009, Journal of forensic sciences,
M Heidarpour Bami, and H R Haddadzadeh, and B Kazemi, and P Khazraiinia, and M Bandehpour, and M Aktas
Molecular identification of three Indian snake species using simple PCR-RFLP method.
July 2010, Journal of forensic sciences,
M Heidarpour Bami, and H R Haddadzadeh, and B Kazemi, and P Khazraiinia, and M Bandehpour, and M Aktas
Identification of different Theileria species (Theileria lestoquardi, Theileria ovis, and Theileria annulata) in naturally infected sheep using nested PCR-RFLP.
April 2011, Parasitology research,
M Heidarpour Bami, and H R Haddadzadeh, and B Kazemi, and P Khazraiinia, and M Bandehpour, and M Aktas
Molecular phylogenetic analysis of new Entoloma rhodopolium-related species in Japan and its identification method using PCR-RFLP.
November 2017, Scientific reports,
M Heidarpour Bami, and H R Haddadzadeh, and B Kazemi, and P Khazraiinia, and M Bandehpour, and M Aktas
PCR-RFLP analysis of mitochondrial DNA: a reliable method for species identification.
April 1999, Journal of agricultural and food chemistry,
M Heidarpour Bami, and H R Haddadzadeh, and B Kazemi, and P Khazraiinia, and M Bandehpour, and M Aktas
Identification of common species of dermatophytes by PCR-RFLP.
January 2005, Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban,
M Heidarpour Bami, and H R Haddadzadeh, and B Kazemi, and P Khazraiinia, and M Bandehpour, and M Aktas
Species identification of trichostrongyle nematodes by PCR-linked RFLP.
April 1994, International journal for parasitology,
M Heidarpour Bami, and H R Haddadzadeh, and B Kazemi, and P Khazraiinia, and M Bandehpour, and M Aktas
A rapid method for identification of typical Leuconostoc species by 16S rDNA PCR-RFLP analysis.
October 2003, Journal of microbiological methods,
Copied contents to your clipboard!