Collagen type V modulates fibroblast behavior dependent on substrate stiffness. 2009

Roel G M Breuls, and Darinka D Klumpers, and Vincent Everts, and Theo H Smit
Department of Physics and Medical Technology, VU University Medical Center, Research Institute MOVE, Van der Boechorststraat 7, P.O. Box 7057, 1007 MB Amsterdam, The Netherlands.

Collagen type V is highly expressed during tissue development and wound repair, but its exact function remains unclear. Cell binding to collagen V affects various basic cell functions and increased collagen V levels alter the structural organization and the stiffness of the ECM. We studied the combined effects of collagen V and substrate stiffness on the morphology, focal adhesion formation, and actin organization of fibroblasts. We found that a hybrid collagen I/V coating impairs fibroblast spreading on soft substrates (<10 kPa), but not on stiffer substrates (68 kPa or glass). In sharp contrast, a pure collagen I coating does not impair cell spreading on soft substrates. The impairment of cell spreading by collagen V is accompanied by diffuse actin staining patterns and small focal adhesions. These observations suggest that collagen V plays an essential role in modifying cell behavior during development and remodeling, when very soft tissues are present.

UI MeSH Term Description Entries
D002465 Cell Movement The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell. Cell Migration,Locomotion, Cell,Migration, Cell,Motility, Cell,Movement, Cell,Cell Locomotion,Cell Motility,Cell Movements,Movements, Cell
D005109 Extracellular Matrix A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere. Matrix, Extracellular,Extracellular Matrices,Matrices, Extracellular
D005347 Fibroblasts Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules. Fibroblast
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000199 Actins Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle. F-Actin,G-Actin,Actin,Isoactin,N-Actin,alpha-Actin,alpha-Isoactin,beta-Actin,gamma-Actin,F Actin,G Actin,N Actin,alpha Actin,alpha Isoactin,beta Actin,gamma Actin
D048430 Cell Shape The quality of surface form or outline of CELLS. Cell Shapes,Shape, Cell,Shapes, Cell
D020100 Hydrogels Water swollen, rigid, 3-dimensional network of cross-linked, hydrophilic macromolecules, 20-95% water. They are used in paints, printing inks, foodstuffs, pharmaceuticals, and cosmetics. (Grant & Hackh's Chemical Dictionary, 5th ed) Hydrogel,In Situ Hydrogel,In Situ Hydrogels,Patterned Hydrogel,Patterned Hydrogels,Hydrogel, In Situ,Hydrogel, Patterned
D022001 Focal Adhesions An anchoring junction of the cell to a non-cellular substrate. It is composed of a specialized area of the plasma membrane where bundles of the ACTIN CYTOSKELETON terminate and attach to the transmembrane linkers, INTEGRINS, which in turn attach through their extracellular domains to EXTRACELLULAR MATRIX PROTEINS. Adhesion Plaques,Cell-Matrix Adherens Junctions,Focal Contacts,Adherens Junction, Cell-Matrix,Adherens Junctions, Cell-Matrix,Adhesion Plaque,Adhesion, Focal,Adhesions, Focal,Cell Matrix Adherens Junctions,Cell-Matrix Adherens Junction,Contact, Focal,Contacts, Focal,Focal Adhesion,Focal Contact,Junction, Cell-Matrix Adherens,Junctions, Cell-Matrix Adherens,Plaque, Adhesion,Plaques, Adhesion
D024062 Collagen Type V A fibrillar collagen found widely distributed as a minor component in tissues that contain COLLAGEN TYPE I and COLLAGEN TYPE III. It is a heterotrimeric molecule composed of alpha1(V), alpha2(V) and alpha3(V) subunits. Several forms of collagen type V exist depending upon the composition of the subunits that form the trimer. Collagen Type V, alpha1 Chain,Collagen Type V, alpha1 Subunit,Collagen Type V, alpha2 Chain,Collagen Type V, alpha2 Subunit,Collagen Type V, alpha3 Chain,Collagen Type V, alpha3 Subunit,Collagen alpha1(V),Collagen alpha2(V),Collagen alpha3(V),Procollagen Type V,Type V Collagen,Type V Procollagen,Collagen, Type V,Procollagen, Type V,V Procollagen, Type

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