Flow cytometric immunophenotyping of peripheral T cell neoplasms using CD3 gating. 2009

Satoshi Hara, and Taiji Yokote, and Toshikazu Akioka, and Takuji Miyoshi, and Toshiyuki Ikemoto, and Hidema Tanaka, and Takayuki Takubo, and Motomu Tsuji, and Toshiaki Hanafusa
Department of Internal Medicine (I), Osaka Medical College, Osaka, Japan. in1211@poh.osaka-med.ac.jp

Peripheral T cell neoplasms (PTCNs) such as peripheral T cell lymphoma, adult T cell leukemia/lymphoma, and mycosis fungoides are associated with poorer prognoses compared to B cell neoplasms. Hence, an accurate and early diagnosis is necessary for the successful treatment of PTCNs; however, this can be difficult to achieve. In this study, flow cytometric immunophenotyping using CD3 gating was performed retrospectively on 56 samples from 52 patients diagnosed with PTCNs, and the analytical data were compared with the immunohistochemical findings. Abnormal CD3 T cell populations were distinguishable from the normal T cell population, using this CD3 gating strategy.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D008297 Male Males
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D005260 Female Females
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012189 Retrospective Studies Studies used to test etiologic hypotheses in which inferences about an exposure to putative causal factors are derived from data relating to characteristics of persons under study or to events or experiences in their past. The essential feature is that some of the persons under study have the disease or outcome of interest and their characteristics are compared with those of unaffected persons. Retrospective Study,Studies, Retrospective,Study, Retrospective
D013601 T-Lymphocytes Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen. T Cell,T Lymphocyte,T-Cells,Thymus-Dependent Lymphocytes,Cell, T,Cells, T,Lymphocyte, T,Lymphocyte, Thymus-Dependent,Lymphocytes, T,Lymphocytes, Thymus-Dependent,T Cells,T Lymphocytes,T-Cell,T-Lymphocyte,Thymus Dependent Lymphocytes,Thymus-Dependent Lymphocyte
D016399 Lymphoma, T-Cell A group of heterogeneous lymphoid tumors representing malignant transformations of T-lymphocytes. T-Cell Lymphoma,Lymphoma, T Cell,Lymphomas, T-Cell,T Cell Lymphoma,T-Cell Lymphomas
D017252 CD3 Complex Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA). Antigens, CD3,CD3 Antigens,T3 Antigens,CD3 Antigen,T3 Antigen,T3 Complex,Antigen, CD3,Antigen, T3,Antigens, T3

Related Publications

Satoshi Hara, and Taiji Yokote, and Toshikazu Akioka, and Takuji Miyoshi, and Toshiyuki Ikemoto, and Hidema Tanaka, and Takayuki Takubo, and Motomu Tsuji, and Toshiaki Hanafusa
February 2007, American journal of clinical pathology,
Satoshi Hara, and Taiji Yokote, and Toshikazu Akioka, and Takuji Miyoshi, and Toshiyuki Ikemoto, and Hidema Tanaka, and Takayuki Takubo, and Motomu Tsuji, and Toshiaki Hanafusa
April 2008, Blood,
Satoshi Hara, and Taiji Yokote, and Toshikazu Akioka, and Takuji Miyoshi, and Toshiyuki Ikemoto, and Hidema Tanaka, and Takayuki Takubo, and Motomu Tsuji, and Toshiaki Hanafusa
April 2000, Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae,
Satoshi Hara, and Taiji Yokote, and Toshikazu Akioka, and Takuji Miyoshi, and Toshiyuki Ikemoto, and Hidema Tanaka, and Takayuki Takubo, and Motomu Tsuji, and Toshiaki Hanafusa
October 2001, American journal of clinical pathology,
Satoshi Hara, and Taiji Yokote, and Toshikazu Akioka, and Takuji Miyoshi, and Toshiyuki Ikemoto, and Hidema Tanaka, and Takayuki Takubo, and Motomu Tsuji, and Toshiaki Hanafusa
May 2024, Annals of laboratory medicine,
Satoshi Hara, and Taiji Yokote, and Toshikazu Akioka, and Takuji Miyoshi, and Toshiyuki Ikemoto, and Hidema Tanaka, and Takayuki Takubo, and Motomu Tsuji, and Toshiaki Hanafusa
January 2013, Annals of hematology,
Satoshi Hara, and Taiji Yokote, and Toshikazu Akioka, and Takuji Miyoshi, and Toshiyuki Ikemoto, and Hidema Tanaka, and Takayuki Takubo, and Motomu Tsuji, and Toshiaki Hanafusa
January 2009, Archives of pathology & laboratory medicine,
Satoshi Hara, and Taiji Yokote, and Toshikazu Akioka, and Takuji Miyoshi, and Toshiyuki Ikemoto, and Hidema Tanaka, and Takayuki Takubo, and Motomu Tsuji, and Toshiaki Hanafusa
February 2018, Acta oto-laryngologica,
Satoshi Hara, and Taiji Yokote, and Toshikazu Akioka, and Takuji Miyoshi, and Toshiyuki Ikemoto, and Hidema Tanaka, and Takayuki Takubo, and Motomu Tsuji, and Toshiaki Hanafusa
December 1996, Cytometry,
Satoshi Hara, and Taiji Yokote, and Toshikazu Akioka, and Takuji Miyoshi, and Toshiyuki Ikemoto, and Hidema Tanaka, and Takayuki Takubo, and Motomu Tsuji, and Toshiaki Hanafusa
March 1993, Annals of the New York Academy of Sciences,
Copied contents to your clipboard!