Biomaterial Database

[Isolation and identification of stem cells derived from human exfoliated deciduous teeth]. 2009

Nuo Xu, and Ke Chen, and Yuan-Yuan Shen

Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou, China. aaronanuo@yahoo.com

OBJECTIVE To isolate and identify stem cells from human exfoliated deciduous teeth (SHED). METHODS Human pulp tissue were dissected and digested to obtain the single cell suspension. The cell morphology was observed and the clonality of the obtained cells was assessed. The phenotype of the cells was detected by immunohistochemistry and flow cytometry (FCM), and the cell cycle was analyzed. The in vitro differentiation of the cells into adipose tissue and formation of mineralization nodules were evaluated. RESULTS Clonogenic assay showed the formation of 16-18 clones in every 10(3) plated cells derived from human exfoliated deciduous teeth. These cells were found to express the markers of mesenchymal stem cells with a multipotent differentiation potential. CONCLUSIONS The cells isolated from human dental pulp are clonogenic and have multipotent differentiation potential, suggesting their identity of SHED.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D002454	Cell Differentiation	Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.	Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D002469	Cell Separation	Techniques for separating distinct populations of cells.	Cell Isolation,Cell Segregation,Isolation, Cell,Cell Isolations,Cell Segregations,Cell Separations,Isolations, Cell,Segregation, Cell,Segregations, Cell,Separation, Cell,Separations, Cell
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D002648	Child	A person 6 to 12 years of age. An individual 2 to 5 years old is CHILD, PRESCHOOL.	Children
D005260	Female		Females
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D014094	Tooth, Deciduous	The teeth of the first dentition, which are shed and replaced by the permanent teeth.	Primary Tooth,Tooth, Primary,Baby Teeth,Baby Tooth,Dentition, Deciduous,Dentition, Primary,Milk Teeth,Milk Tooth,Primary Teeth,Teeth, Deciduous,Teeth, Primary,Deciduous Dentition,Deciduous Dentitions,Deciduous Teeth,Deciduous Tooth,Dentitions, Deciduous,Dentitions, Primary,Primary Dentition,Primary Dentitions,Teeth, Baby,Teeth, Milk,Tooth, Baby,Tooth, Milk
D039902	Multipotent Stem Cells	Specialized stem cells that are committed to give rise to cells that have a particular function; examples are MYOBLASTS; MYELOID PROGENITOR CELLS; and skin stem cells. (Stem Cells: A Primer [Internet]. Bethesda (MD): National Institutes of Health (US); 2000 May [cited 2002 Apr 5]. Available from: http://www.nih.gov/news/stemcell/primer.htm)	Stem Cells, Multipotent,Multipotent Stem Cell,Stem Cell, Multipotent