Heterogeneity of S-layer proteins from aggregating and non-aggregating Lactobacillus kefir strains. 2009

Pablo Mobili, and María de Los Angeles Serradell, and Sebastián A Trejo, and Francesc X Avilés Puigvert, and Analía G Abraham, and Graciela L De Antoni
Centro de Investigación y Desarrollo en Criotecnología de Alimentos, CCT La Plata, CONICET, 47 y 116 (s/n), La Plata, Argentina.

Since the presence of S-layer protein conditioned the autoaggregation capacity of some strains of Lactobacillus kefir, S-layer proteins from aggregating and non-aggregating L. kefir strains were characterized by immunochemical reactivity, MALDI-TOF spectrometry and glycosylation analysis. Two anti-S-layer monoclonal antibodies (Mab5F8 and Mab1F8) were produced; in an indirect enzyme-linked immunosorbent assay Mab1F8 recognized S-layer proteins from all L. kefir tested while Mab5F8 recognized only S-layer proteins from aggregating strains. Periodic Acid-Schiff staining of proteins after polyacrylamide gel electrophoresis under denaturing conditions revealed that all L. kefir S-layer proteins tested were glycosylated. Growth of bacteria in the presence of the N-glycosylation inhibitor tunicamycin suggested the presence of glycosydic chains O-linked to the protein backbone. MALDI-TOF peptide map fingerprint for S-layer proteins from 12 L. kefir strains showed very similar patterns for the aggregating strains, different from those for the non-aggregating ones. No positive match with other protein spectra in MSDB Database was found. Our results revealed a high heterogeneity among S-layer proteins from different L. kefir strains but also suggested a correlation between the structure of these S-layer glycoproteins and the aggregation properties of whole bacterial cells.

UI MeSH Term Description Entries
D007778 Lactobacillus A genus of gram-positive, microaerophilic, rod-shaped bacteria occurring widely in nature. Its species are also part of the many normal flora of the mouth, intestinal tract, and vagina of many mammals, including humans. Lactobacillus species are homofermentative and ferment a broad spectrum of carbohydrates often host-adapted but do not ferment PENTOSES. Most members were previously assigned to the Lactobacillus delbrueckii group. Pathogenicity from this genus is rare.
D008562 Membrane Glycoproteins Glycoproteins found on the membrane or surface of cells. Cell Surface Glycoproteins,Surface Glycoproteins,Cell Surface Glycoprotein,Membrane Glycoprotein,Surface Glycoprotein,Glycoprotein, Cell Surface,Glycoprotein, Membrane,Glycoprotein, Surface,Glycoproteins, Cell Surface,Glycoproteins, Membrane,Glycoproteins, Surface,Surface Glycoprotein, Cell,Surface Glycoproteins, Cell
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D006031 Glycosylation The synthetic chemistry reaction or enzymatic reaction of adding carbohydrate or glycosyl groups. GLYCOSYLTRANSFERASES carry out the enzymatic glycosylation reactions. The spontaneous, non-enzymatic attachment of reducing sugars to free amino groups in proteins, lipids, or nucleic acids is called GLYCATION (see MAILLARD REACTION). Protein Glycosylation,Glycosylation, Protein
D000907 Antibodies, Bacterial Immunoglobulins produced in a response to BACTERIAL ANTIGENS. Bacterial Antibodies
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D043302 Cultured Milk Products Derivatives of milk that are produced by controlled FERMENTATION. Fermented Dairy Products,Fermented Milk Products,Milk Products, Cultured,Cultured Milk Product,Dairy Product, Fermented,Dairy Products, Fermented,Fermented Dairy Product,Fermented Milk Product,Milk Product, Cultured,Milk Product, Fermented,Milk Products, Fermented,Product, Cultured Milk,Product, Fermented Dairy,Product, Fermented Milk,Products, Cultured Milk,Products, Fermented Dairy,Products, Fermented Milk
D019032 Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis. Laser Desorption-Ionization Mass Spectrometry, Matrix-Assisted,MALD-MS,MALDI,Mass Spectrometry, Matrix-Assisted Laser Desorption-Ionization,Mass Spectroscopy, Matrix-Assisted Laser Desorption-Ionization,Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry,Spectroscopy, Mass, Matrix-Assisted Laser Desorption-Ionization,MALDI-MS,MS-MALD,SELDI-TOF-MS,Surface Enhanced Laser Desorption Ionization Mass Spectrometry,Laser Desorption Ionization Mass Spectrometry, Matrix Assisted,MALDI MS,Mass Spectrometry, Matrix Assisted Laser Desorption Ionization,Mass Spectroscopy, Matrix Assisted Laser Desorption Ionization,Matrix Assisted Laser Desorption Ionization Mass Spectrometry

Related Publications

Pablo Mobili, and María de Los Angeles Serradell, and Sebastián A Trejo, and Francesc X Avilés Puigvert, and Analía G Abraham, and Graciela L De Antoni
January 1992, Microbiology and immunology,
Pablo Mobili, and María de Los Angeles Serradell, and Sebastián A Trejo, and Francesc X Avilés Puigvert, and Analía G Abraham, and Graciela L De Antoni
June 2018, Journal of liposome research,
Pablo Mobili, and María de Los Angeles Serradell, and Sebastián A Trejo, and Francesc X Avilés Puigvert, and Analía G Abraham, and Graciela L De Antoni
February 2014, Anaerobe,
Pablo Mobili, and María de Los Angeles Serradell, and Sebastián A Trejo, and Francesc X Avilés Puigvert, and Analía G Abraham, and Graciela L De Antoni
April 2015, World journal of microbiology & biotechnology,
Pablo Mobili, and María de Los Angeles Serradell, and Sebastián A Trejo, and Francesc X Avilés Puigvert, and Analía G Abraham, and Graciela L De Antoni
February 2019, Microbiology (Reading, England),
Pablo Mobili, and María de Los Angeles Serradell, and Sebastián A Trejo, and Francesc X Avilés Puigvert, and Analía G Abraham, and Graciela L De Antoni
June 2004, Die Nahrung,
Pablo Mobili, and María de Los Angeles Serradell, and Sebastián A Trejo, and Francesc X Avilés Puigvert, and Analía G Abraham, and Graciela L De Antoni
May 2004, The Journal of dairy research,
Pablo Mobili, and María de Los Angeles Serradell, and Sebastián A Trejo, and Francesc X Avilés Puigvert, and Analía G Abraham, and Graciela L De Antoni
January 2013, PloS one,
Pablo Mobili, and María de Los Angeles Serradell, and Sebastián A Trejo, and Francesc X Avilés Puigvert, and Analía G Abraham, and Graciela L De Antoni
November 1995, FEMS microbiology letters,
Pablo Mobili, and María de Los Angeles Serradell, and Sebastián A Trejo, and Francesc X Avilés Puigvert, and Analía G Abraham, and Graciela L De Antoni
November 2005, Applied and environmental microbiology,
Copied contents to your clipboard!