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Plasma 17alpha OH progesterone was assayed radioimmunologically, using an anti-17-OH-progesterone BSA antiserum as specific binding antigen. The method consisted in extracting steroids with ethyl ether from 1 ml plasm (to which 17alpha OH progesterone-1,2-3H had been added for the assessment of losses); the extract was then submitted to chromatography on a Sephadex LH-20 column, antiserum was added and the free steroid was separated from the bound form by Charcoal-dextran. Average recovery of 17alpha OH progesterone-1,2-3H was 75+/-5%. Minimum sensitivity of the method oscillated about 7-10 pg. MIF (Method Interfering Factors) were evaluated. The method was used for the assay of 17alpha OH progesterone plasma levels during normal menstrual cycles, in women undergoing ovarian stimulation with HMG and HCG in the follicular and luteal phase respectively. Finally, changes of plasma 17alpha OH progesterone levels were assessed during adrenal stimulation and suppression in various stages of the menstrual cycle.
V M Jasonni, and
C Melega, and
G Possati, and
M S Preti, and
P Crea, and
S Lodi, and
C Flamigni
October 1976,
Journal of steroid biochemistry,
V M Jasonni, and
C Melega, and
G Possati, and
M S Preti, and
P Crea, and
S Lodi, and
C Flamigni
March 1977,
Nihon Naibunpi Gakkai zasshi,
V M Jasonni, and
C Melega, and
G Possati, and
M S Preti, and
P Crea, and
S Lodi, and
C Flamigni
January 1964,
Research on steroids,
V M Jasonni, and
C Melega, and
G Possati, and
M S Preti, and
P Crea, and
S Lodi, and
C Flamigni
January 1960,
Klinische Wochenschrift,
V M Jasonni, and
C Melega, and
G Possati, and
M S Preti, and
P Crea, and
S Lodi, and
C Flamigni
May 1969,
Acta endocrinologica,
V M Jasonni, and
C Melega, and
G Possati, and
M S Preti, and
P Crea, and
S Lodi, and
C Flamigni
September 1968,
The Journal of physiology,
V M Jasonni, and
C Melega, and
G Possati, and
M S Preti, and
P Crea, and
S Lodi, and
C Flamigni
January 1979,
The Journal of nuclear medicine and allied sciences,
V M Jasonni, and
C Melega, and
G Possati, and
M S Preti, and
P Crea, and
S Lodi, and
C Flamigni
March 1969,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
V M Jasonni, and
C Melega, and
G Possati, and
M S Preti, and
P Crea, and
S Lodi, and
C Flamigni
March 1974,
Hormones and behavior,
V M Jasonni, and
C Melega, and
G Possati, and
M S Preti, and
P Crea, and
S Lodi, and
C Flamigni
October 2007,
Reproduction in domestic animals = Zuchthygiene,
