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3H-Spiroperidol association and dissociation rate constants were determined in rat striatal homogenates in the presence of known concentrations of unlabelled haloperidol. The rate of 3H-spiroperidol association was progressively decreased in the presence of increasing concentrations of haloperidol and no changes were seen in 3H-spiroperidol dissociation rate constants. Measuring changes in 3H-spiroperidol association rate constants permitted detection of 0.1 pmol/ml unlabelled haloperidol in assay homogenates. Then, male Sprague-Dawley rats received daily injections of haloperidol (1.5 mg/kg) for 1-21 days. Twenty-four hours after the last injection, 3H-spiroperidol kinetic rate constants and Kd values were measured and found to be unchanged in all treatment groups. These findings suggest that, under these conditions of drug withdrawal and tissue preparation, which are widely employed in studies of chronic neuroleptic administration, residual haloperidol does not interfere with the estimation of 3H-spiroperidol binding parameters.
E P Bellows, and
D E Barnes, and
J G Csernansky
November 1991,
Neuroscience research,
E P Bellows, and
D E Barnes, and
J G Csernansky
October 1999,
European journal of pharmacology,
E P Bellows, and
D E Barnes, and
J G Csernansky
January 1982,
Life sciences,
E P Bellows, and
D E Barnes, and
J G Csernansky
December 1992,
Clinical neuropharmacology,
E P Bellows, and
D E Barnes, and
J G Csernansky
January 1979,
Brain research,
E P Bellows, and
D E Barnes, and
J G Csernansky
August 1997,
Experimental neurology,
E P Bellows, and
D E Barnes, and
J G Csernansky
March 1992,
Brain research,
E P Bellows, and
D E Barnes, and
J G Csernansky
August 2002,
Synapse (New York, N.Y.),
E P Bellows, and
D E Barnes, and
J G Csernansky
January 1990,
Brain research,
E P Bellows, and
D E Barnes, and
J G Csernansky
May 1992,
The Journal of pharmacology and experimental therapeutics,
