Cloning and characterization of hypusine-containing protein eIF5A from the olive flounder Paralichthys olivaceus. 2009

Hee Jeong Kong, and Gyeong-Eun Hong, and Woo-Jin Kim, and Young-Ok Kim, and Bo-Hye Nam, and Chang Hoon Lee, and Jeong Wan Do, and Jeong-Ho Lee, and Sang-Jun Lee, and Kyung-Kil Kim
Biotechnology Research Institute, National Fisheries Research and Development Institute, 408-1 Sirang-ri, Gijang-up, Gijang-gun, Busan 619-705, Republic of Korea. konghj@nfrdi.go.kr

Eukaryotic translation initiation factor 5A (eIF5A) is the only protein in eukaryotic cells that contains the unusual amino acid hypusine (N(epsilon)-(4-amino-2(R)-hydroxybutyl)-lysine). We isolated a 1385-bp eIF5A cDNA containing an open reading frame (ORF) of 468 bp, which encodes a protein of 155 amino acids with a conserved hypusine modification site, from the olive flounder Paralichthys olivaceus. Pairwise alignments revealed that flounder eIF5A had a high sequence identity with those of other known species including mammals. Real-time RT-PCR analysis showed the expression of eIF5A mRNA was constitutively detected in various tissues of healthy flounder. In HINAE cells or flounder kidney infected with the viral hemorrhagic septicemia virus (VHSV), the expression of eIF5A mRNA was slightly increased before cells showed cytopathic effects and then decreased when cells showed cytopathic effects. Treatment of N-guanyl-1,7-diaminoheptane (GC-7), a potent inhibitor of eIF5A hypusination, inhibited the expression of VHSV G protein in a dose-dependent manner suggesting a potential role for eIF5A and its hypusination in viral protein expression.

UI MeSH Term Description Entries
D007668 Kidney Body organ that filters blood for the secretion of URINE and that regulates ion concentrations. Kidneys
D008239 Lysine An essential amino acid. It is often added to animal feed. Enisyl,L-Lysine,Lysine Acetate,Lysine Hydrochloride,Acetate, Lysine,L Lysine
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010448 Peptide Initiation Factors Protein factors uniquely required during the initiation phase of protein synthesis in GENETIC TRANSLATION. Initiation Factors,Initiation Factor,Factors, Peptide Initiation,Initiation Factors, Peptide
D010802 Phylogeny The relationships of groups of organisms as reflected by their genetic makeup. Community Phylogenetics,Molecular Phylogenetics,Phylogenetic Analyses,Phylogenetic Analysis,Phylogenetic Clustering,Phylogenetic Comparative Analysis,Phylogenetic Comparative Methods,Phylogenetic Distance,Phylogenetic Generalized Least Squares,Phylogenetic Groups,Phylogenetic Incongruence,Phylogenetic Inference,Phylogenetic Networks,Phylogenetic Reconstruction,Phylogenetic Relatedness,Phylogenetic Relationships,Phylogenetic Signal,Phylogenetic Structure,Phylogenetic Tree,Phylogenetic Trees,Phylogenomics,Analyse, Phylogenetic,Analysis, Phylogenetic,Analysis, Phylogenetic Comparative,Clustering, Phylogenetic,Community Phylogenetic,Comparative Analysis, Phylogenetic,Comparative Method, Phylogenetic,Distance, Phylogenetic,Group, Phylogenetic,Incongruence, Phylogenetic,Inference, Phylogenetic,Method, Phylogenetic Comparative,Molecular Phylogenetic,Network, Phylogenetic,Phylogenetic Analyse,Phylogenetic Clusterings,Phylogenetic Comparative Analyses,Phylogenetic Comparative Method,Phylogenetic Distances,Phylogenetic Group,Phylogenetic Incongruences,Phylogenetic Inferences,Phylogenetic Network,Phylogenetic Reconstructions,Phylogenetic Relatednesses,Phylogenetic Relationship,Phylogenetic Signals,Phylogenetic Structures,Phylogenetic, Community,Phylogenetic, Molecular,Phylogenies,Phylogenomic,Reconstruction, Phylogenetic,Relatedness, Phylogenetic,Relationship, Phylogenetic,Signal, Phylogenetic,Structure, Phylogenetic,Tree, Phylogenetic
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D005432 Flounder Common name for two families of FLATFISHES belonging to the order Pleuronectiformes: left-eye flounders (Bothidae) and right-eye flounders (Pleuronectidae). The latter is more commonly used in research. Plaice,Platichthys,Pleuronectes,Pseudopleuronectes,Halibut,Pleuronectes platessa,Flounders
D006023 Glycoproteins Conjugated protein-carbohydrate compounds including MUCINS; mucoid, and AMYLOID glycoproteins. C-Glycosylated Proteins,Glycosylated Protein,Glycosylated Proteins,N-Glycosylated Proteins,O-Glycosylated Proteins,Glycoprotein,Neoglycoproteins,Protein, Glycosylated,Proteins, C-Glycosylated,Proteins, Glycosylated,Proteins, N-Glycosylated,Proteins, O-Glycosylated
D006147 Guanine

Related Publications

Hee Jeong Kong, and Gyeong-Eun Hong, and Woo-Jin Kim, and Young-Ok Kim, and Bo-Hye Nam, and Chang Hoon Lee, and Jeong Wan Do, and Jeong-Ho Lee, and Sang-Jun Lee, and Kyung-Kil Kim
May 2008, Fish & shellfish immunology,
Hee Jeong Kong, and Gyeong-Eun Hong, and Woo-Jin Kim, and Young-Ok Kim, and Bo-Hye Nam, and Chang Hoon Lee, and Jeong Wan Do, and Jeong-Ho Lee, and Sang-Jun Lee, and Kyung-Kil Kim
November 2010, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology,
Hee Jeong Kong, and Gyeong-Eun Hong, and Woo-Jin Kim, and Young-Ok Kim, and Bo-Hye Nam, and Chang Hoon Lee, and Jeong Wan Do, and Jeong-Ho Lee, and Sang-Jun Lee, and Kyung-Kil Kim
July 2013, Applied biochemistry and biotechnology,
Hee Jeong Kong, and Gyeong-Eun Hong, and Woo-Jin Kim, and Young-Ok Kim, and Bo-Hye Nam, and Chang Hoon Lee, and Jeong Wan Do, and Jeong-Ho Lee, and Sang-Jun Lee, and Kyung-Kil Kim
October 2008, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology,
Hee Jeong Kong, and Gyeong-Eun Hong, and Woo-Jin Kim, and Young-Ok Kim, and Bo-Hye Nam, and Chang Hoon Lee, and Jeong Wan Do, and Jeong-Ho Lee, and Sang-Jun Lee, and Kyung-Kil Kim
December 2009, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology,
Hee Jeong Kong, and Gyeong-Eun Hong, and Woo-Jin Kim, and Young-Ok Kim, and Bo-Hye Nam, and Chang Hoon Lee, and Jeong Wan Do, and Jeong-Ho Lee, and Sang-Jun Lee, and Kyung-Kil Kim
October 2009, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology,
Hee Jeong Kong, and Gyeong-Eun Hong, and Woo-Jin Kim, and Young-Ok Kim, and Bo-Hye Nam, and Chang Hoon Lee, and Jeong Wan Do, and Jeong-Ho Lee, and Sang-Jun Lee, and Kyung-Kil Kim
January 2011, Fish & shellfish immunology,
Hee Jeong Kong, and Gyeong-Eun Hong, and Woo-Jin Kim, and Young-Ok Kim, and Bo-Hye Nam, and Chang Hoon Lee, and Jeong Wan Do, and Jeong-Ho Lee, and Sang-Jun Lee, and Kyung-Kil Kim
February 2010, Veterinary immunology and immunopathology,
Hee Jeong Kong, and Gyeong-Eun Hong, and Woo-Jin Kim, and Young-Ok Kim, and Bo-Hye Nam, and Chang Hoon Lee, and Jeong Wan Do, and Jeong-Ho Lee, and Sang-Jun Lee, and Kyung-Kil Kim
September 2009, General and comparative endocrinology,
Hee Jeong Kong, and Gyeong-Eun Hong, and Woo-Jin Kim, and Young-Ok Kim, and Bo-Hye Nam, and Chang Hoon Lee, and Jeong Wan Do, and Jeong-Ho Lee, and Sang-Jun Lee, and Kyung-Kil Kim
June 2008, BMC immunology,
Copied contents to your clipboard!