BIOMAT Database Logo BIOMAT Database Logo

Cholesterol ester accumulation in cultured aortic smooth muscle cells. Induction of cholesterol ester retention by chloroquine and low density lipoprotein and its reversion by mixtures of high density apolipoprotein and sphingomyelin. 1977

O Stein, and J Vanderhoek, and Y Stein

Accretion of cholesterol ester was studied in rat aortic smooth muscle cells in culture. Confluent multilayers of smooth muscle cells were exposed to human low density lipoprotein (LDL) and chloroquine and this treatment resulted in a very marked increase in cellular cholesterol ester. The degree of enrichment in cholesterol ester was related inversely to the cell density in the petri dish and was maximal in 48 h. The morphological changes after 48 h incubation with chloroquine and LDL consisted of accumulation of numerous membrane-bound inclusions containing electron-dense and electron-lucent material, some of which resembled secondary lysosomes. These changes resembled some of the changes observed in human and experimental atheromatosis. Similar inclusions were seen also in cultured human skin fibroblasts which accumulated large amounts of cholesterol ester during 48 h incubation with LDL and chloroquine. Removal of the accumulated cellular cholesterol ester was studied in the two cell types and it was markedly enhanced in the presence of lipoprotein-deficient serum and high density apolipoprotein-sphingomyelin mixture. The morphological findings after 24 h of post incubation revealed the presence of empty vacuoles, membrane whorls and cytoplasmic lipid droplets. The present results indicate that aortic smooth muscle cells in culture can serve as a good model to study the role of the lysosomal system in atherogenesis.

UI MeSH Term Description Entries
D008077 Lipoproteins, LDL A class of lipoproteins of small size (18-25 nm) and light (1.019-1.063 g/ml) particles with a core composed mainly of CHOLESTEROL ESTERS and smaller amounts of TRIGLYCERIDES. The surface monolayer consists mostly of PHOSPHOLIPIDS, a single copy of APOLIPOPROTEIN B-100, and free cholesterol molecules. The main LDL function is to transport cholesterol and cholesterol esters to extrahepatic tissues. Low-Density Lipoprotein,Low-Density Lipoproteins,beta-Lipoprotein,beta-Lipoproteins,LDL(1),LDL(2),LDL-1,LDL-2,LDL1,LDL2,Low-Density Lipoprotein 1,Low-Density Lipoprotein 2,LDL Lipoproteins,Lipoprotein, Low-Density,Lipoproteins, Low-Density,Low Density Lipoprotein,Low Density Lipoprotein 1,Low Density Lipoprotein 2,Low Density Lipoproteins,beta Lipoprotein,beta Lipoproteins
D008297 Male Males
D009130 Muscle, Smooth Unstriated and unstriped muscle, one of the muscles of the internal organs, blood vessels, hair follicles, etc. Contractile elements are elongated, usually spindle-shaped cells with centrally located nuclei. Smooth muscle fibers are bound together into sheets or bundles by reticular fibers and frequently elastic nets are also abundant. (From Stedman, 25th ed) Muscle, Involuntary,Smooth Muscle,Involuntary Muscle,Involuntary Muscles,Muscles, Involuntary,Muscles, Smooth,Smooth Muscles
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D002738 Chloroquine The prototypical antimalarial agent with a mechanism that is not well understood. It has also been used to treat rheumatoid arthritis, systemic lupus erythematosus, and in the systemic therapy of amebic liver abscesses. Aralen,Arechine,Arequin,Chingamin,Chlorochin,Chloroquine Sulfate,Chloroquine Sulphate,Khingamin,Nivaquine,Sulfate, Chloroquine,Sulphate, Chloroquine
D002784 Cholesterol The principal sterol of all higher animals, distributed in body tissues, especially the brain and spinal cord, and in animal fats and oils. Epicholesterol
D002788 Cholesterol Esters Fatty acid esters of cholesterol which constitute about two-thirds of the cholesterol in the plasma. The accumulation of cholesterol esters in the arterial intima is a characteristic feature of atherosclerosis. Cholesterol Ester,Cholesteryl Ester,Cholesteryl Esters,Ester, Cholesterol,Ester, Cholesteryl,Esters, Cholesterol,Esters, Cholesteryl
D005260 Female Females
D005347 Fibroblasts Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules. Fibroblast
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

O Stein, and J Vanderhoek, and Y Stein
Retro-endocytosis of low density lipoprotein by cultured bovine aortic smooth muscle cells.
May 1981, Biochimica et biophysica acta,
O Stein, and J Vanderhoek, and Y Stein
Low-density lipoprotein uptake and cholesterol accumulation by cultured renal cells.
December 1991, Journal of the American Society of Nephrology : JASN,
O Stein, and J Vanderhoek, and Y Stein
Macrophages enhance binding of beta-VLDL and cholesterol ester accumulation in cultured aortic smooth muscle cells.
January 1994, Heart and vessels,
O Stein, and J Vanderhoek, and Y Stein
Platelet-mediated cholesterol accumulation in cultured aortic smooth muscle cells.
March 1985, Science (New York, N.Y.),
O Stein, and J Vanderhoek, and Y Stein
Degradation of apolipoprotein B of low density lipoprotein by cultured bovine smooth muscle cells. Accumulation of intermediates in the presence of protease inhibitors.
February 1981, Biochimica et biophysica acta,
O Stein, and J Vanderhoek, and Y Stein
Intimal smooth muscle cells up-regulate beta-very low density lipoprotein-mediated cholesterol accumulation by enhancing beta-very low density lipoprotein uptake and decreasing cholesterol efflux.
November 2002, Biochimica et biophysica acta,
O Stein, and J Vanderhoek, and Y Stein
Cholesterol accumulation in aortic smooth muscle cells exposed to low density lipoproteins. Contribution of free cholesterol transfer.
January 1988, Arteriosclerosis (Dallas, Tex.),
O Stein, and J Vanderhoek, and Y Stein
Herpesvirus infection enhances cholesterol and cholesteryl ester accumulation in cultured arterial smooth muscle cells.
November 1981, The American journal of pathology,
O Stein, and J Vanderhoek, and Y Stein
Reliability of low-density lipoprotein cholesterol, non-high-density lipoprotein cholesterol, and apolipoprotein B measurement.
January 2011, Journal of clinical lipidology,
O Stein, and J Vanderhoek, and Y Stein
Cholesterol esterification and cholesteryl ester accumulation in cultured pigeon and monkey arterial smooth muscle cells.
April 1979, Experimental and molecular pathology,
Copied contents to your clipboard!