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A simple and reliable reversed-phase high-performance liquid chromatographic method with coulometric detection is described for the quantitation of naltrexone and its metabolite, 6 beta-naltrexol, in plasma samples of healthy volunteers who received orally 50 mg of naltrexone. The analytes and the internal standard, naloxone, are extracted with an octadecyl solid-phase extraction column before chromatography. The mobile phase is 0.01 M potassium phosphate (pH 3)-acetonitrile (85:15, v/v) and it is pumped at 0.8 ml/min. The coulometric detector is formed by two electrodes set at +0.20 V and +0.70 V, with a palladium reference electrode. The limit of quantitation observed was 5 ng/ml for both naltrexone and 6 beta-naltrexol. This method can be used to investigate pharmacokinetic parameters of different pharmaceutical preparations of this opioid antagonist.
P Zuccaro, and
I Altieri, and
P Betto, and
R Pacifici, and
G Ricciarello, and
L A Pini, and
E Sternieri, and
S Pichini
September 1996,
Journal of pharmaceutical and biomedical analysis,
P Zuccaro, and
I Altieri, and
P Betto, and
R Pacifici, and
G Ricciarello, and
L A Pini, and
E Sternieri, and
S Pichini
October 1993,
Journal of chromatography,
P Zuccaro, and
I Altieri, and
P Betto, and
R Pacifici, and
G Ricciarello, and
L A Pini, and
E Sternieri, and
S Pichini
April 1990,
Journal of chromatography,
P Zuccaro, and
I Altieri, and
P Betto, and
R Pacifici, and
G Ricciarello, and
L A Pini, and
E Sternieri, and
S Pichini
August 2012,
BMC research notes,
P Zuccaro, and
I Altieri, and
P Betto, and
R Pacifici, and
G Ricciarello, and
L A Pini, and
E Sternieri, and
S Pichini
April 1996,
Journal of chromatography. B, Biomedical applications,
P Zuccaro, and
I Altieri, and
P Betto, and
R Pacifici, and
G Ricciarello, and
L A Pini, and
E Sternieri, and
S Pichini
January 1995,
Journal of chromatography. B, Biomedical applications,
P Zuccaro, and
I Altieri, and
P Betto, and
R Pacifici, and
G Ricciarello, and
L A Pini, and
E Sternieri, and
S Pichini
January 1993,
Journal of pharmaceutical and biomedical analysis,
P Zuccaro, and
I Altieri, and
P Betto, and
R Pacifici, and
G Ricciarello, and
L A Pini, and
E Sternieri, and
S Pichini
January 1980,
Journal of analytical toxicology,
P Zuccaro, and
I Altieri, and
P Betto, and
R Pacifici, and
G Ricciarello, and
L A Pini, and
E Sternieri, and
S Pichini
January 1981,
NIDA research monograph,
P Zuccaro, and
I Altieri, and
P Betto, and
R Pacifici, and
G Ricciarello, and
L A Pini, and
E Sternieri, and
S Pichini
May 1986,
Journal of chromatography,
