Biomaterial Database

Detection and differentiation of antibodies to human T-cell lymphotropic virus types I and II by the immunofluorescence method. 1991

D Gallo, and L M Penning, and C V Hanson

Viral and Rickettsial Disease Laboratory, State of California Department of Health Services, Berkeley 94704.

We compared the sensitivities of the prototype human T-cell lymphotropic virus type I (HTLV-I)- and HTLV-II-transformed cell lines, MT2 and Mo-T, with that of an HTLV-II-infected cell line, clone 19, established in our laboratory, in the immunofluorescence (IF) test for detection of antibody to HTLV-I and HTLV-II. In addition, IF antibody titers with the three antigens were determined, and the results were compared with HTLV-I and HTLV-II typing by polymerase chain reaction (PCR). The MT2 cell line was more sensitive than the two HTLV-II cell lines for detecting HTLV-I antibody by IF, and clone 19 was more sensitive than Mo-T or MT2 for measuring HTLV-II antibody. In the titration study, the antigen that gave the highest titer correlated completely with the HTLV type determined by PCR, indicating that the relatively simple IF titration method can be used for differentiating HTLV-I and HTLV-II antibody in sera and plasmas.

UI	MeSH Term	Description	Entries
D002461	Cell Line, Transformed	Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals.	Transformed Cell Line,Cell Lines, Transformed,Transformed Cell Lines
D005069	Evaluation Studies as Topic	Works about studies that determine the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies.	Critique,Evaluation Indexes,Evaluation Methodology,Evaluation Report,Evaluation Research,Methodology, Evaluation,Pre-Post Tests,Qualitative Evaluation,Quantitative Evaluation,Theoretical Effectiveness,Use-Effectiveness,Critiques,Effectiveness, Theoretical,Evaluation Methodologies,Evaluation Reports,Evaluation, Qualitative,Evaluation, Quantitative,Evaluations, Qualitative,Evaluations, Quantitative,Indexes, Evaluation,Methodologies, Evaluation,Pre Post Tests,Pre-Post Test,Qualitative Evaluations,Quantitative Evaluations,Report, Evaluation,Reports, Evaluation,Research, Evaluation,Test, Pre-Post,Tests, Pre-Post,Use Effectiveness
D005455	Fluorescent Antibody Technique	Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.	Antinuclear Antibody Test, Fluorescent,Coon's Technique,Fluorescent Antinuclear Antibody Test,Fluorescent Protein Tracing,Immunofluorescence Technique,Coon's Technic,Fluorescent Antibody Technic,Immunofluorescence,Immunofluorescence Technic,Antibody Technic, Fluorescent,Antibody Technics, Fluorescent,Antibody Technique, Fluorescent,Antibody Techniques, Fluorescent,Coon Technic,Coon Technique,Coons Technic,Coons Technique,Fluorescent Antibody Technics,Fluorescent Antibody Techniques,Fluorescent Protein Tracings,Immunofluorescence Technics,Immunofluorescence Techniques,Protein Tracing, Fluorescent,Protein Tracings, Fluorescent,Technic, Coon's,Technic, Fluorescent Antibody,Technic, Immunofluorescence,Technics, Fluorescent Antibody,Technics, Immunofluorescence,Technique, Coon's,Technique, Fluorescent Antibody,Technique, Immunofluorescence,Techniques, Fluorescent Antibody,Techniques, Immunofluorescence,Tracing, Fluorescent Protein,Tracings, Fluorescent Protein
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D015367	Human T-lymphotropic virus 2	A strain of PRIMATE T-LYMPHOTROPIC VIRUS 2 that can transform normal T-lymphocytes and can replicate in both T- and B-cell lines. The virus is related to but distinct from HTLV-1.	HTLV-2,HTLV-II,Human T-Cell Leukemia Virus II,Human T-Lymphotropic Virus II,Leukemia Virus II, Human T-Cell,T-Cell Leukemia Virus II, Human,Human T Cell Leukemia Virus II,Human T Lymphotropic Virus II,Leukemia Virus II, Human T Cell,T Cell Leukemia Virus II, Human,Human T lymphotropic virus 2
D015368	Human T-lymphotropic virus 1	A strain of PRIMATE T-LYMPHOTROPIC VIRUS 1 isolated from mature T4 cells in patients with T-lymphoproliferation malignancies. It causes adult T-cell leukemia (LEUKEMIA-LYMPHOMA, T-CELL, ACUTE, HTLV-I-ASSOCIATED), T-cell lymphoma (LYMPHOMA, T-CELL), and is involved in mycosis fungoides, SEZARY SYNDROME and tropical spastic paraparesis (PARAPARESIS, TROPICAL SPASTIC).	ATLV,Adult T-Cell Leukemia-Lymphoma Virus I,HTLV-1,HTLV-I,Human T-Cell Leukemia Virus I,Leukemia Virus I, Human T-Cell,T-Cell Leukemia Virus I, Human,Adult T Cell Leukemia Lymphoma Virus I,Human T Cell Leukemia Virus I,Leukemia Lymphoma Virus I, Adult T Cell,Leukemia Virus I, Human T Cell,T Cell Leukemia Virus I, Human,Human T lymphotropic virus 1
D015481	HTLV-I Antibodies	Antibodies reactive with the HTLV-I ANTIGENS.	ATLV Antibodies,Human T-Cell Leukemia Virus I Antibodies,Leukemia-Lymphoma Virus I Antibodies, Human T-Cell,T-Cell Leukemia Virus I Antibodies, Adult,T-Cell Leukemia Virus I Antibodies, Human,HTLV I Antibodies,Human T Cell Leukemia Virus I Antibodies,Leukemia Lymphoma Virus I Antibodies, Human T Cell,T Cell Leukemia Virus I Antibodies, Adult,T Cell Leukemia Virus I Antibodies, Human,Antibodies, ATLV,Antibodies, HTLV I,Antibodies, HTLV-I,I Antibodies, HTLV
D015482	HTLV-II Antibodies	Antibodies reactive with the HTLV-II ANTIGENS.	Human T-Cell Leukemia Virus II Antibodies,Human T-Lymphotropic Virus II Antibodies,Leukemia-Lymphoma Virus II Antibodies, Human T-Cell,T-Cell Leukemia Virus II Antibodies, Human,HTLV II Antibodies,Human T Cell Leukemia Virus II Antibodies,Human T Lymphotropic Virus II Antibodies,Leukemia Lymphoma Virus II Antibodies, Human T Cell,T Cell Leukemia Virus II Antibodies, Human,Antibodies, HTLV II,Antibodies, HTLV-II,II Antibodies, HTLV
D015486	HTLV-I Antigens	Antigens associated with HUMAN T-LYMPHOTROPIC VIRUS 1.	ATLV Antigens,Human T-Cell Leukemia Virus I Antigens,Leukemia-Lymphoma Virus I Antigens, Human T-Cell,T-Cell Leukemia Virus I Antigens, Adult,T-Cell Leukemia Virus I Antigens, Human,HTLV I Antigens,Human T Cell Leukemia Virus I Antigens,Leukemia Lymphoma Virus I Antigens, Human T Cell,T Cell Leukemia Virus I Antigens, Adult,T Cell Leukemia Virus I Antigens, Human,Antigens, ATLV,Antigens, HTLV I,Antigens, HTLV-I,I Antigens, HTLV
D015487	HTLV-II Antigens	Antigens associated with HUMAN T-LYMPHOTROPIC VIRUS 2.	Human T-Cell Leukemia Virus II Antigens,Human T-Lymphotropic Virus II Antigens,Leukemia-Lymphoma Virus II Antigens, Human T-Cell,T-Cell Leukemia Virus II Antigens, Human,HTLV II Antigens,Human T Cell Leukemia Virus II Antigens,Human T Lymphotropic Virus II Antigens,Leukemia Lymphoma Virus II Antigens, Human T Cell,T Cell Leukemia Virus II Antigens, Human,Antigens, HTLV II,Antigens, HTLV-II,II Antigens, HTLV

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