BIOMAT Database Logo BIOMAT Database Logo

Separation of X- and Y-bearing human spermatozoa by sperm isolation medium gradients evaluated by FISH. 2009

F Aleahmad, and H Gourabi, and B Zeinali, and S Kazemi Ashtiani, and H Baharvand
University of Tehran, University College of Science, School of Biology, Royan Institute ACECR, Tehran, Iran.

An eight-layer discontinuous sperm isolation medium (PureSperm gradient) was evaluated in separation of human spermatozoa according to sex chromosomes by fluorescence in-situ hybridization (FISH). This study was carried out on spare samples from normozoospermic and oligozoospermic patients referred to the Royan Institute for infertility treatment. Semen analysis was assessed according to World Health Organization criteria. X- and Y-bearing spermatozoa were simultaneously identified in the neat semen (control) and sperm isolation medium fractions from the same samples using FISH and chromosome specific DNA probes. At least 1000 spermatozoa were scored for each sample. The proportions of X- and Y-bearing spermatozoa were determined by presence of red or green fluorescent signals. Before separation, there was no significant difference in the percentage of spermatozoa with the specific signals of X and Y chromosomes. After separation, in both normal and oligozoospermic patients, the percentage of X-bearing spermatozoa in the bottom layer slightly exceeded that in the top layer (P = 0.001). In both the normal and oligozoospermic groups, the difference between the frequencies of Y-bearing spermatozoa in the top and bottom layers was significant (P = 0.001). It seemed that eight-step discontinuous gradient was not a reliable method for the separation X- and Y-bearing spermatozoa for clinical purposes.

UI MeSH Term Description Entries
D008297 Male Males
D002469 Cell Separation Techniques for separating distinct populations of cells. Cell Isolation,Cell Segregation,Isolation, Cell,Cell Isolations,Cell Segregations,Cell Separations,Isolations, Cell,Segregation, Cell,Segregations, Cell,Separation, Cell,Separations, Cell
D002499 Centrifugation, Density Gradient Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Centrifugations, Density Gradient,Density Gradient Centrifugation,Density Gradient Centrifugations,Gradient Centrifugation, Density,Gradient Centrifugations, Density
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013094 Spermatozoa Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility. Sperm,Spermatozoon,X-Bearing Sperm,X-Chromosome-Bearing Sperm,Y-Bearing Sperm,Y-Chromosome-Bearing Sperm,Sperm, X-Bearing,Sperm, X-Chromosome-Bearing,Sperm, Y-Bearing,Sperm, Y-Chromosome-Bearing,Sperms, X-Bearing,Sperms, X-Chromosome-Bearing,Sperms, Y-Bearing,Sperms, Y-Chromosome-Bearing,X Bearing Sperm,X Chromosome Bearing Sperm,X-Bearing Sperms,X-Chromosome-Bearing Sperms,Y Bearing Sperm,Y Chromosome Bearing Sperm,Y-Bearing Sperms,Y-Chromosome-Bearing Sperms
D017404 In Situ Hybridization, Fluorescence A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei. FISH Technique,Fluorescent in Situ Hybridization,Hybridization in Situ, Fluorescence,FISH Technic,Hybridization in Situ, Fluorescent,In Situ Hybridization, Fluorescent,FISH Technics,FISH Techniques,Technic, FISH,Technics, FISH,Technique, FISH,Techniques, FISH
D041321 Chromosomes, Human, X The human female sex chromosome, being the differential sex chromosome carried by half the male gametes and all female gametes in humans. X Chromosome, Human,Chromosome, Human X,Chromosomes, Human X,Human X Chromosome,Human X Chromosomes,X Chromosomes, Human
D041322 Chromosomes, Human, Y The human male sex chromosome, being the differential sex chromosome carried by half the male gametes and none of the female gametes in humans. Y Chromosome, Human,Chromosome, Human Y,Chromosomes, Human Y,Human Y Chromosome,Human Y Chromosomes,Y Chromosomes, Human

Related Publications

F Aleahmad, and H Gourabi, and B Zeinali, and S Kazemi Ashtiani, and H Baharvand
Separation of X- and Y-bearing human spermatozoa using albumin gradients.
October 1994, Human reproduction (Oxford, England),
F Aleahmad, and H Gourabi, and B Zeinali, and S Kazemi Ashtiani, and H Baharvand
Detection of X- and Y-bearing human spermatozoa after motile sperm isolation by swim-up.
December 1993, Fertility and sterility,
F Aleahmad, and H Gourabi, and B Zeinali, and S Kazemi Ashtiani, and H Baharvand
Failure to confirm separation of X- and Y-bearing human sperm using BSA gradients.
January 1975, Nature,
F Aleahmad, and H Gourabi, and B Zeinali, and S Kazemi Ashtiani, and H Baharvand
Galvanic separation of X- and Y-bearing human spermatozoa.
January 1974, Andrologia,
F Aleahmad, and H Gourabi, and B Zeinali, and S Kazemi Ashtiani, and H Baharvand
Galvanic separation of X- and Y-bearing human spermatozoa.
January 1975, International journal of fertility,
F Aleahmad, and H Gourabi, and B Zeinali, and S Kazemi Ashtiani, and H Baharvand
Separation of human X and Y spermatozoa by albumin gradients and Sephadex chromatography.
January 1982, Fertility and sterility,
F Aleahmad, and H Gourabi, and B Zeinali, and S Kazemi Ashtiani, and H Baharvand
[Galvanic separation of X- and Y-bearing human spermatozoa (author's transl)].
May 1974, Nihon Hinyokika Gakkai zasshi. The japanese journal of urology,
F Aleahmad, and H Gourabi, and B Zeinali, and S Kazemi Ashtiani, and H Baharvand
[Separation of human X- and Y- chromosome-bearing spermatozoa by column chromatography (author's transl)].
January 1979, Dermatologische Monatschrift,
F Aleahmad, and H Gourabi, and B Zeinali, and S Kazemi Ashtiani, and H Baharvand
Electrophoretic separation of X- and Y-chromosome-bearing sperm in human semen.
July 1974, The Tohoku journal of experimental medicine,
F Aleahmad, and H Gourabi, and B Zeinali, and S Kazemi Ashtiani, and H Baharvand
Albumin gradients do not enrich Y-bearing human spermatozoa.
May 1997, Human reproduction (Oxford, England),
Copied contents to your clipboard!