BIOMAT Database Logo BIOMAT Database Logo

Thermotolerance and HSP70 expression in the Mediterranean fruit fly Ceratitis capitata. 2009

Katerina Kalosaka, and Elisavet Soumaka, and Nikos Politis, and Anastassios C Mintzas
Division of Genetics, Cell and Developmental Biology, Department of Biology, University of Patras, Patras, Greece.

The relationship between Hsp70 expression and thermotolerance has been well documented in Drosophila melanogaster. However, there is limited information on this relationship in other insect species. In this report we describe the Hsp70-thermotolerance relationship in one of the major fruit fly pests, Ceratitis capitata (medfly). Hsp70 expression and thermotolerance were assayed at a range of temperatures in several stages of medfly development. The most thermotolerant stage was found to be the late larval stage (100% survival at 41 degrees C) followed by adult flies and late embryos (100% survival at 39 degrees C). These three stages showed a positive relationship between Hsp70 expression and thermotolerance. Mid-larval and mid-embryonic stages were found less thermotolerant and the Hsp70-thermotolerance relationship was not evident. Early embryos did not express Hsp70 at any temperature and exhibited the lowest thermotolerance. The relationship between Hsp70 and inducible thermotolerance was also studied in late larvae. A pretreatment at 37-39 degrees C increased thermotolerance at higher temperatures by approximately 1 degrees C. In parallel, the pretreatment increased Hsp70 expression suggesting a close link between Hsp70 expression and inducible thermotolerance. The increased Hsp70 levels after pretreatment were found to be due to the increased levels of the hsp70 RNA.

UI MeSH Term Description Entries
D007814 Larva Wormlike or grublike stage, following the egg in the life cycle of insects, worms, and other metamorphosing animals. Maggots,Tadpoles,Larvae,Maggot,Tadpole
D006358 Hot Temperature Presence of warmth or heat or a temperature notably higher than an accustomed norm. Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D015870 Gene Expression The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION. Expression, Gene,Expressions, Gene,Gene Expressions
D018840 HSP70 Heat-Shock Proteins A class of MOLECULAR CHAPERONES found in both prokaryotes and in several compartments of eukaryotic cells. These proteins can interact with polypeptides during a variety of assembly processes in such a way as to prevent the formation of nonfunctional structures. Heat-Shock Proteins 70,Heat Shock 70 kDa Protein,Heat-Shock Protein 70,HSP70 Heat Shock Proteins,Heat Shock Protein 70,Heat Shock Proteins 70,Heat-Shock Proteins, HSP70
D019476 Insect Proteins Proteins found in any species of insect. Insect Protein,Protein, Insect,Proteins, Insect
D033622 Ceratitis capitata A species of fruit fly originating in sub-Saharan Africa but widely distributed worldwide. One of the most destructive fruit pests, its larvae feed and develop on many different fruits and some vegetables. Fruit Fly, Mediterranean,Medfly,Mediterranean Fruit Fly,Ceratitis capitatas,Flies, Mediterranean Fruit,Fly, Mediterranean Fruit,Fruit Flies, Mediterranean,Medflies,Mediterranean Fruit Flies,capitatas, Ceratitis

Related Publications

Katerina Kalosaka, and Elisavet Soumaka, and Nikos Politis, and Anastassios C Mintzas
A member of the hsp70 gene family from the Mediterranean fruit fly, Ceratitis capitata.
February 1998, Insect molecular biology,
Katerina Kalosaka, and Elisavet Soumaka, and Nikos Politis, and Anastassios C Mintzas
Microsatellite polymorphism in the Mediterranean fruit fly, Ceratitis capitata.
June 2000, Insect molecular biology,
Katerina Kalosaka, and Elisavet Soumaka, and Nikos Politis, and Anastassios C Mintzas
Molecular sexing in the Mediterranean fruit fly, Ceratitis capitata.
February 2004, Insect biochemistry and molecular biology,
Katerina Kalosaka, and Elisavet Soumaka, and Nikos Politis, and Anastassios C Mintzas
Actin genes in the Mediterranean fruit fly, Ceratitis capitata.
May 1990, Genetics,
Katerina Kalosaka, and Elisavet Soumaka, and Nikos Politis, and Anastassios C Mintzas
Heat shock proteins and thermotolerance in a cultured cell line from the Mediterranean fruit fly, Ceratitis capitata.
January 1992, Archives of insect biochemistry and physiology,
Katerina Kalosaka, and Elisavet Soumaka, and Nikos Politis, and Anastassios C Mintzas
The mitochondrial genome of the mediterranean fruit fly, Ceratitis capitata.
April 2000, Insect molecular biology,
Katerina Kalosaka, and Elisavet Soumaka, and Nikos Politis, and Anastassios C Mintzas
Susceptibility of the Mediterranean fruit fly (Ceratitis capitata) and the Natal fruit fly (Ceratitis rosa) to entomopathogenic nematodes.
January 2009, Journal of invertebrate pathology,
Katerina Kalosaka, and Elisavet Soumaka, and Nikos Politis, and Anastassios C Mintzas
Mitochondrial DNA restriction map for the Mediterranean fruit fly, Ceratitis capitata.
February 1994, Biochemical genetics,
Katerina Kalosaka, and Elisavet Soumaka, and Nikos Politis, and Anastassios C Mintzas
Sniffing out chemosensory genes from the Mediterranean fruit fly, Ceratitis capitata.
January 2014, PloS one,
Katerina Kalosaka, and Elisavet Soumaka, and Nikos Politis, and Anastassios C Mintzas
Molecular cloning and expression of nanos in the Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae).
January 2013, Gene expression patterns : GEP,
Copied contents to your clipboard!