Experience with a new agar culture medium for demonstrating the presence of anaerobic bacteria. 1975

K Vágújhelyi, and L Zalay, and L Hegedüs

In view of the fact that anaerobic diagnostics have become a matter of primary importance, the authors have developed the RCM medium with certain modifications. Owing to its good keeping quality, this medium can be used also on smaller laboratories for demonstrating the presence of anaerobic bacteria and for carrying out germ counts. It constitutes and adequate base for the anaerobic blood medium and for the Wilson-Blair medium, and also for the determination of the antibiotic resistance of the anaerobic bacteria. The sensitivity of this medium surpasses that of the thioglycollate medium, and is suitable in its carbohydrate-free form also for testing the strains for their sugar-fermentative capactiy. Our work was aimed at serving the anaerobic diagnostics of clinical laboratories, and at serving at the same time the method of demonstrating the presence of anaerobes becoming increasingle important in the food industry.

UI MeSH Term Description Entries
D003016 Clostridium perfringens The most common etiologic agent of GAS GANGRENE. It is differentiable into several distinct types based on the distribution of twelve different toxins. Clostridium welchii
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D006089 Gram-Negative Anaerobic Bacteria A large group of anaerobic bacteria which show up as pink (negative) when treated by the Gram-staining method. Gram Negative Anaerobic Bacteria
D000362 Agar A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.
D013864 Thioglycolates Organic esters of thioglycolic acid (HS-CH2COOH). Thioglycollates

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