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Immunization of rabbits with a pooled preparation of chromato-graphically purified avian myeloblastosis virus (AMV) group-specific (gs) antigens produced relatively large volumes of antiserum that was as broadly reactive in complement-fixation (CF) tests as antiserum produced in hamsters with tumors induced by Rous sarcoma virus. This alternate procedure should be of value for routine preparation of leukosis virus gs antiserum. Other antisera prepared against disrupted AMV had spurious reactivities that confused CF testing of embryos and tissue extracts. This artifact was attributed to the high affinity of serum albumin for AMV virions and the resultant production of antibodies when plasma-derived AMV was used as immunogen. A high-molecular-weight AMV CF antigen was also found in early fractions eluted from an agarose-gel column.
E J Smith
January 1970,
Neoplasma,
E J Smith
February 1966,
Proceedings of the National Academy of Sciences of the United States of America,
E J Smith
January 1970,
Bibliotheca haematologica,
E J Smith
January 1975,
Voprosy virusologii,
E J Smith
June 1969,
Virology,
E J Smith
January 1973,
Monatshefte fur Veterinarmedizin,
E J Smith
September 1972,
Monatshefte fur Veterinarmedizin,
E J Smith
August 1985,
The Journal of general virology,
E J Smith
May 1969,
American journal of veterinary research,
