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[Gas chromatography-mass spectrometric analysis of Jatropha curcas leaf extracts prepared by supercritical fluid CO2 extraction]. 2009

Zhao-yu Wang, and Fei-long Chen, and Jing-ming Lin, and Shu-lin Huang
School of Life Science and Biopharmacology, Guangdong Pharmaceutical University, and Department of Pharmaceutics, Zhujiang Hospital, Guangzhou 510006, China. wangzhaoyu77@yahoo.com.cn

OBJECTIVE To analyze the bioactive components in Jatropha curcas leaves using gas chromatography-mass spectrometry (GC-MS). METHODS The bioactive components were extracted from J. curcas leaves by supercritical fluid CO2 extraction and analyzed by using GC-MS. RESULTS Seventy peaks were detected by GC-MS, and 43 compounds were identified (61.43%). Among the identified compounds, 16 had a content of more than 1%, and the total contents of these 16 compounds reached 81.36%. The four most abundant components were 22,23-dihydro-stigmasterol (16.14%), alpha-tocopherol (15.18%), beta-amylin (7.73%) and dotriacontanol (7.02%). The content of gamma-tocopherol reached 2.88% and vitamin E reached 18.06% in the extract. CONCLUSIONS J. curcas leaves contain multiple compounds with anti-tumor, anti-virus and antimicrobial activities.

UI MeSH Term Description Entries
D008401 Gas Chromatography-Mass Spectrometry A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds. Chromatography, Gas-Liquid-Mass Spectrometry,Chromatography, Gas-Mass Spectrometry,GCMS,Spectrometry, Mass-Gas Chromatography,Spectrum Analysis, Mass-Gas Chromatography,Gas-Liquid Chromatography-Mass Spectrometry,Mass Spectrometry-Gas Chromatography,Chromatography, Gas Liquid Mass Spectrometry,Chromatography, Gas Mass Spectrometry,Chromatography, Mass Spectrometry-Gas,Chromatography-Mass Spectrometry, Gas,Chromatography-Mass Spectrometry, Gas-Liquid,Gas Chromatography Mass Spectrometry,Gas Liquid Chromatography Mass Spectrometry,Mass Spectrometry Gas Chromatography,Spectrometries, Mass-Gas Chromatography,Spectrometry, Gas Chromatography-Mass,Spectrometry, Gas-Liquid Chromatography-Mass,Spectrometry, Mass Gas Chromatography,Spectrometry-Gas Chromatography, Mass,Spectrum Analysis, Mass Gas Chromatography
D010936 Plant Extracts Concentrated pharmaceutical preparations of plants obtained by removing active constituents with a suitable solvent, which is evaporated away, and adjusting the residue to a prescribed standard. Herbal Medicines,Plant Extract,Extract, Plant,Extracts, Plant,Medicines, Herbal
D018515 Plant Leaves Expanded structures, usually green, of vascular plants, characteristically consisting of a bladelike expansion attached to a stem, and functioning as the principal organ of photosynthesis and transpiration. (American Heritage Dictionary, 2d ed) Plant Leaf,Leaf, Plant,Leave, Plant,Leaves, Plant,Plant Leave
D025924 Chromatography, Supercritical Fluid A CHROMATOGRAPHY method using supercritical fluid, usually carbon dioxide under very high pressure (around 73 atmospheres or 1070 psi at room temperature) as the mobile phase. Other solvents are sometimes added as modifiers. This is used both for analytical (SFC) and extraction (SFE) purposes. Supercritical Fluid Chromatography,Supercritical Fluid Extraction,Extraction, Supercritical Fluid,SFC (Supercritical Fluid Chromatography),SFE (Supercritical Fluid Extraction),Fluid Chromatography, Supercritical,Fluid Extraction, Supercritical
D031286 Jatropha A plant genus of the family EUPHORBIACEAE. Members contain jatrophone and other diterpenes. Nettlespurge,Spurge, Nettle,Jatropha curcas,Physic Nut,Purging Nut,Nettle Spurge,Nettle Spurges,Nettlespurges,Nut, Physic,Nut, Purging,Nuts, Physic,Nuts, Purging,Physic Nuts,Purging Nuts,Spurges, Nettle

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