Seven healthy male volunteers participated in four different heavy cross-country ski trips in the mountains, 1 year apart, wearing a back-pack weighing 30 kg. Each trip lasted for 8 days covering a total distance of 160 km. Serum triglycerides and lipoprotein cholesterol were measured before and after the trip. The different experiments were carried out with the same participants and under the same conditions, but with a varying diet composition. With the four different diets used, a standard diet (3800 kcal day-1, 26% fat, 260 mg cholesterol day-1), a low-energy diet (2300 kcal day-1, 21% fat, 110 mg cholesterol day-1, a high-fat-high-cholesterol diet (3800 kcal day-1, 52% fat, 480 mg cholesterol day-1) and a high-cholesterol diet (3800 kcal day-1, 29% fat, 410 mg cholesterol day-1), a decrease in very low-density lipoprotein-low-density lipoprotein (VLDL-LDL) fraction by 38%, 50%, 41%, and 54%, respectively, was obtained. No significant increase in high-density lipoprotein (HDL) cholesterol was found in the experiments with the standard diet and the low-energy diet, but in the high-fat-high-cholesterol diet experiment the HDL cholesterol increased by 19% and the ratio HDL cholesterol:total cholesterol from 0.327 to 0.490. With the high-cholesterol diet an increase in HDL cholesterol of about the same extent was obtained. In all experiments the serum triglycerides decreased by more than 30% and no significant difference for the different diets was obtained.(ABSTRACT TRUNCATED AT 250 WORDS)