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A modified micro solid-phase radioimmunoassay (RIA) for antibody to hepatitis A virus (anti-HAV) was developed. This double antibody procedure was performed by coating the surface of a polyvinyl microtiter plate "well" with 200 microliter of a 1:1,000 dilution of a patient's test serum. Purified HAV and 125I-labeled immunoglobulin G (IgG) anti-HAV were then sequentially added to form an antibody sandwich. The specificity and sensitivity of the RIA procedure for anti-HAV were verified by examination of coded human and chimpanzee serum specimens. Radioimmunoassay of early-acute-phase serum specimens from human cases of hepatitis A revealed the presence of anti-HAV activity. Differential examination by RIA of IgG and IgM fractions of acute-phase sera from experimentally infected chimpanzees demonstrated that IgM contained the bulk of the anti-HAV activity. A modification of the RIA procedure for anti-HAV (RIA-IgM blocking), incorporating an incubation step with anti-IgM (Mu chain specific), was further shown to differentiate acute- from convalescent-phase hepatitis A sera. This adapted RIA-IgM blocking procedure required less than 1 microliter of a single acute-phase serum specimen for the diagnosis of viral hepatitis A.
D W Bradley, and
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C L Hornbeck, and
H A Fields, and
K A McCaustland, and
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April 1981,
Acta medica Okayama,
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June 1980,
Infection and immunity,
D W Bradley, and
J E Maynard, and
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C L Hornbeck, and
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K A McCaustland, and
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January 1981,
Scandinavian journal of infectious diseases,
D W Bradley, and
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May 2006,
Journal of gastroenterology and hepatology,
D W Bradley, and
J E Maynard, and
S H Hindman, and
C L Hornbeck, and
H A Fields, and
K A McCaustland, and
E H Cook
November 1982,
Journal of clinical microbiology,
D W Bradley, and
J E Maynard, and
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C L Hornbeck, and
H A Fields, and
K A McCaustland, and
E H Cook
August 1976,
Journal of clinical microbiology,
D W Bradley, and
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C L Hornbeck, and
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K A McCaustland, and
E H Cook
June 2013,
European journal of gastroenterology & hepatology,
D W Bradley, and
J E Maynard, and
S H Hindman, and
C L Hornbeck, and
H A Fields, and
K A McCaustland, and
E H Cook
January 1983,
Hepatology (Baltimore, Md.),
D W Bradley, and
J E Maynard, and
S H Hindman, and
C L Hornbeck, and
H A Fields, and
K A McCaustland, and
E H Cook
April 1979,
Journal of clinical microbiology,
D W Bradley, and
J E Maynard, and
S H Hindman, and
C L Hornbeck, and
H A Fields, and
K A McCaustland, and
E H Cook
April 1986,
Journal of clinical microbiology,
