BIOMAT Database Logo BIOMAT Database Logo

Novel alpha-fetoprotein-V messenger RNA isoforms in humans. 2009

Hikaru Tagaya, and Hiroko Fukasawa, and Tomoko Shoda, and Kazuhiko Hoshi, and Shuji Hirata
Department of Obstetrics and Gynecology, University of Yamanashi, Shimokato, Yamanashi, Japan.

It has been demonstrated that several messenger RNA (mRNA) isoforms have been transcribed from the alpha-fetoprotein (AFP) gene. In rats, it was reported that the novel exon, termed the exon V, exists between the exons 7 and 8, and the novel mRNA isoform (termed AFP-V mRNA) is synthesized using the exon V. In this study, a reverse transcription-polymerase chain reaction was performed and quantitative analysis was done on the AFP mRNA to identify the exon V and the AFP-V mRNA in humans. As a result, 2 novel exons, the exons VA and VB, were identified. Furthermore, 3 novel AFP mRNAs, the AFP-V1, -V2, and -V3 mRNA, were demonstrated to be expressed through alternative splicing. Expression of the AFP-V2 mRNA isoform and the wild-type mRNA was differentially regulated, implying that the AFP-V mRNA isoforms could be used in diagnosis and classification of hepatocellular carcinoma and ovarian carcinoma.

UI MeSH Term Description Entries
D008113 Liver Neoplasms Tumors or cancer of the LIVER. Cancer of Liver,Hepatic Cancer,Liver Cancer,Cancer of the Liver,Cancer, Hepatocellular,Hepatic Neoplasms,Hepatocellular Cancer,Neoplasms, Hepatic,Neoplasms, Liver,Cancer, Hepatic,Cancer, Liver,Cancers, Hepatic,Cancers, Hepatocellular,Cancers, Liver,Hepatic Cancers,Hepatic Neoplasm,Hepatocellular Cancers,Liver Cancers,Liver Neoplasm,Neoplasm, Hepatic,Neoplasm, Liver
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010051 Ovarian Neoplasms Tumors or cancer of the OVARY. These neoplasms can be benign or malignant. They are classified according to the tissue of origin, such as the surface EPITHELIUM, the stromal endocrine cells, and the totipotent GERM CELLS. Cancer of Ovary,Ovarian Cancer,Cancer of the Ovary,Neoplasms, Ovarian,Ovary Cancer,Ovary Neoplasms,Cancer, Ovarian,Cancer, Ovary,Cancers, Ovarian,Cancers, Ovary,Neoplasm, Ovarian,Neoplasm, Ovary,Neoplasms, Ovary,Ovarian Cancers,Ovarian Neoplasm,Ovary Cancers,Ovary Neoplasm
D005091 Exons The parts of a transcript of a split GENE remaining after the INTRONS are removed. They are spliced together to become a MESSENGER RNA or other functional RNA. Mini-Exon,Exon,Mini Exon,Mini-Exons
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000230 Adenocarcinoma A malignant epithelial tumor with a glandular organization. Adenocarcinoma, Basal Cell,Adenocarcinoma, Granular Cell,Adenocarcinoma, Oxyphilic,Adenocarcinoma, Tubular,Adenoma, Malignant,Carcinoma, Cribriform,Carcinoma, Granular Cell,Carcinoma, Tubular,Adenocarcinomas,Adenocarcinomas, Basal Cell,Adenocarcinomas, Granular Cell,Adenocarcinomas, Oxyphilic,Adenocarcinomas, Tubular,Adenomas, Malignant,Basal Cell Adenocarcinoma,Basal Cell Adenocarcinomas,Carcinomas, Cribriform,Carcinomas, Granular Cell,Carcinomas, Tubular,Cribriform Carcinoma,Cribriform Carcinomas,Granular Cell Adenocarcinoma,Granular Cell Adenocarcinomas,Granular Cell Carcinoma,Granular Cell Carcinomas,Malignant Adenoma,Malignant Adenomas,Oxyphilic Adenocarcinoma,Oxyphilic Adenocarcinomas,Tubular Adenocarcinoma,Tubular Adenocarcinomas,Tubular Carcinoma,Tubular Carcinomas
D000509 alpha-Fetoproteins The first alpha-globulins to appear in mammalian sera during FETAL DEVELOPMENT and the dominant serum proteins in early embryonic life. alpha-Fetoprotein,alpha Fetoprotein,alpha Fetoproteins
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated

Related Publications

Hikaru Tagaya, and Hiroko Fukasawa, and Tomoko Shoda, and Kazuhiko Hoshi, and Shuji Hirata
Changes in polysomal polyadenylated RNA and alpha-fetoprotein messenger RNA during hepatocarcinogenesis.
October 1980, Cancer research,
Hikaru Tagaya, and Hiroko Fukasawa, and Tomoko Shoda, and Kazuhiko Hoshi, and Shuji Hirata
Stability of alpha-fetoprotein messenger RNA in mouse yolk sac.
January 1982, Developmental biology,
Hikaru Tagaya, and Hiroko Fukasawa, and Tomoko Shoda, and Kazuhiko Hoshi, and Shuji Hirata
Isolation of alpha-fetoprotein messenger RNA from mouse yolk sac.
June 1979, The Journal of biological chemistry,
Hikaru Tagaya, and Hiroko Fukasawa, and Tomoko Shoda, and Kazuhiko Hoshi, and Shuji Hirata
The elevation of alpha-fetoprotein messenger RNA in regenerating rat liver.
January 1981, Biochemical and biophysical research communications,
Hikaru Tagaya, and Hiroko Fukasawa, and Tomoko Shoda, and Kazuhiko Hoshi, and Shuji Hirata
Tissue specificity of alpha-fetoprotein messenger RNA expression during mouse embryogenesis.
January 1983, The EMBO journal,
Hikaru Tagaya, and Hiroko Fukasawa, and Tomoko Shoda, and Kazuhiko Hoshi, and Shuji Hirata
Quantitation of alpha-fetoprotein and albumin messenger RNA in human hepatocellular carcinoma.
June 1996, Hepatology (Baltimore, Md.),
Hikaru Tagaya, and Hiroko Fukasawa, and Tomoko Shoda, and Kazuhiko Hoshi, and Shuji Hirata
APC gene messenger RNA: novel isoforms that lack exon 7.
December 1993, Cancer research,
Hikaru Tagaya, and Hiroko Fukasawa, and Tomoko Shoda, and Kazuhiko Hoshi, and Shuji Hirata
Quantitation of rat alpha-fetoprotein messenger RNA with a complementary DNA probe.
December 1977, The Journal of biological chemistry,
Hikaru Tagaya, and Hiroko Fukasawa, and Tomoko Shoda, and Kazuhiko Hoshi, and Shuji Hirata
Liver regeneration and alpha-fetoprotein messenger RNA expression in the retrorsine model for hepatocyte transplantation.
December 1998, Cancer research,
Hikaru Tagaya, and Hiroko Fukasawa, and Tomoko Shoda, and Kazuhiko Hoshi, and Shuji Hirata
Association between recurrence of hepatocellular carcinoma and alpha-fetoprotein messenger RNA levels in peripheral blood.
January 2005, Surgery today,
Copied contents to your clipboard!