BIOMAT Database Logo BIOMAT Database Logo

Single-step protein purification by back flush in ion exchange chromatography. 2009

Ming-Kai Chern, and Wei-Jyh Shiah, and Jyun-Jie Chen, and Tzung-You Tsai, and Hsin-Yin Lin, and Chien-Wei Liu
Graduate Institute of Life Sciences, Tamkang University, Tamsui 25137, Taiwan. mkchern@mail.tku.edu.tw

Ion exchange chromatography, one of the major procedures for protein purification, seldom provides single-step purification due to a lack of specific affinity. In this work, a novel and simple method called "back flush" (i.e., reversing the flow direction of elution relative to that of sample loading) was developed to achieve single-step purification on an ion exchanger. Tips for the conditions and operation by back flush are presented. Our study demonstrates, for the first time, the feasibility and dramatic improvement for protein purification by the back-flush method.

UI MeSH Term Description Entries
D011994 Recombinant Proteins Proteins prepared by recombinant DNA technology. Biosynthetic Protein,Biosynthetic Proteins,DNA Recombinant Proteins,Recombinant Protein,Proteins, Biosynthetic,Proteins, Recombinant DNA,DNA Proteins, Recombinant,Protein, Biosynthetic,Protein, Recombinant,Proteins, DNA Recombinant,Proteins, Recombinant,Recombinant DNA Proteins,Recombinant Proteins, DNA
D002852 Chromatography, Ion Exchange Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins. Chromatography, Ion-Exchange,Ion-Exchange Chromatography,Chromatographies, Ion Exchange,Chromatographies, Ion-Exchange,Ion Exchange Chromatographies,Ion Exchange Chromatography,Ion-Exchange Chromatographies
D006863 Hydrogen-Ion Concentration The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000445 Aldehyde Oxidoreductases Oxidoreductases that are specific for ALDEHYDES. Aldehyde Oxidoreductase,Oxidoreductase, Aldehyde,Oxidoreductases, Aldehyde
D012441 Saccharomyces cerevisiae A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement. Baker's Yeast,Brewer's Yeast,Candida robusta,S. cerevisiae,Saccharomyces capensis,Saccharomyces italicus,Saccharomyces oviformis,Saccharomyces uvarum var. melibiosus,Yeast, Baker's,Yeast, Brewer's,Baker Yeast,S cerevisiae,Baker's Yeasts,Yeast, Baker
D020690 GTPase-Activating Proteins Proteins that activate the GTPase of specific GTP-BINDING PROTEINS. GAP Proteins,GAP Protein,GTPase-Activating Protein,GTPase Activating Protein,GTPase Activating Proteins
D029701 Saccharomyces cerevisiae Proteins Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes. Baker's Yeast Proteins,S cerevisiae Proteins

Related Publications

Ming-Kai Chern, and Wei-Jyh Shiah, and Jyun-Jie Chen, and Tzung-You Tsai, and Hsin-Yin Lin, and Chien-Wei Liu
Purification of transfer RNA species by single-step ion-exchange high-performance liquid chromatography.
July 1988, Journal of chromatography,
Ming-Kai Chern, and Wei-Jyh Shiah, and Jyun-Jie Chen, and Tzung-You Tsai, and Hsin-Yin Lin, and Chien-Wei Liu
Single-step ion exchange purification of the coagulant protein from Moringa oleifera seed.
May 2006, Applied microbiology and biotechnology,
Ming-Kai Chern, and Wei-Jyh Shiah, and Jyun-Jie Chen, and Tzung-You Tsai, and Hsin-Yin Lin, and Chien-Wei Liu
[Separation and purification of PEGylated rhG-CSF by two-step ion-exchange chromatography].
March 2005, Sheng wu gong cheng xue bao = Chinese journal of biotechnology,
Ming-Kai Chern, and Wei-Jyh Shiah, and Jyun-Jie Chen, and Tzung-You Tsai, and Hsin-Yin Lin, and Chien-Wei Liu
Purification of bradykinin by ion-exchange chromatography.
December 1956, The Biochemical journal,
Ming-Kai Chern, and Wei-Jyh Shiah, and Jyun-Jie Chen, and Tzung-You Tsai, and Hsin-Yin Lin, and Chien-Wei Liu
Purification of erythropoietin by ion-exchange chromatography.
July 1958, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
Ming-Kai Chern, and Wei-Jyh Shiah, and Jyun-Jie Chen, and Tzung-You Tsai, and Hsin-Yin Lin, and Chien-Wei Liu
Antibody purification: ion-exchange chromatography.
January 2010, Methods in molecular biology (Clifton, N.J.),
Ming-Kai Chern, and Wei-Jyh Shiah, and Jyun-Jie Chen, and Tzung-You Tsai, and Hsin-Yin Lin, and Chien-Wei Liu
One-step purification of mouse monoclonal antibodies by mass ion exchange chromatography on Zetaprep.
May 1987, Journal of immunological methods,
Ming-Kai Chern, and Wei-Jyh Shiah, and Jyun-Jie Chen, and Tzung-You Tsai, and Hsin-Yin Lin, and Chien-Wei Liu
Three step purification of C1q by DNA precipitation, ion exchange and lectin affinity chromatography.
October 1982, Journal of clinical pathology,
Ming-Kai Chern, and Wei-Jyh Shiah, and Jyun-Jie Chen, and Tzung-You Tsai, and Hsin-Yin Lin, and Chien-Wei Liu
The purification of trypsinogen by ion-exchange chromatography.
May 1962, Canadian journal of biochemistry and physiology,
Ming-Kai Chern, and Wei-Jyh Shiah, and Jyun-Jie Chen, and Tzung-You Tsai, and Hsin-Yin Lin, and Chien-Wei Liu
Purification of complex protein mixtures by ion-exchange displacement chromatography using spacer displacers.
June 1992, Journal of chromatography,
Copied contents to your clipboard!