Biomaterial Database

[Damage on lung and systemic inflammation induced by nickel sulfate intratracheal instillation in rats]. 2009

Furong Deng, and Xinbiao Guo, and Pingping Xia, and Hong Liu

Department of Occupational & Environmental Health Science, School of Public Health, Peking University, Beijing 100191, China. frdeng@yahoo.com.cn

OBJECTIVE To investigate the acute toxic effects induced by nickel sulfate (NiSO4) in order to clarify the possible role of nickel in PM2.5. METHODS Twenty-eight male Wistar rats were divided into four groups, NiSO4 was administered by intratracheal instillation at the doses of Ni 0, 7.5, 75 and 750 microg/kg for 24h respectively. The rats were sacrificed and bronchoalveolar lavage fluid (BALF) were collected and analyzed to estimate the injury (total protein (TP) and lactate dehydrogenase (LDH)) and inflammation of lung. Blood were collected and parameters of inflammation (total cell numbers, interleukin-6 (IL-6), tumor necrosis factor (TNF-alpha)) and CRP were measured. The nickel levels in the blood were also determined. RESULTS NiSO4 induced significant increases of leukocytes, neutrophil, TP contents and LDH activity in BALF (P < 0.05, P < 0.01). The level of TNF-alpha and IL-6 in BALF of NiSO4-treated rats increased significantly compared with those of the control group (P < 0.05). The level of TNF-alpha in blood of NiSO4-treated rats increased significantly compared with the control group. Concentrations of CRP in the serum were more higher in NiSO4-treated rats than the control group, but statistical significances were only seen in the highest dosage group (Ni 750 microg/kg). Nickel in serum of NiSO4-treated rats increased significantly in a dose-dependent manner. CONCLUSIONS NiSO4 induced pulmonary and systemic inflammation effects in rats, suggested that NiSO4 may play an important role in the acute effects of PM2.5.

UI	MeSH Term	Description	Entries
D007249	Inflammation	A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.	Innate Inflammatory Response,Inflammations,Inflammatory Response, Innate,Innate Inflammatory Responses
D007442	Intubation, Intratracheal	A procedure involving placement of a tube into the trachea through the mouth or nose in order to provide a patient with oxygen and anesthesia.	Intubation, Endotracheal,Endotracheal Intubation,Endotracheal Intubations,Intratracheal Intubation,Intratracheal Intubations,Intubations, Endotracheal,Intubations, Intratracheal
D008168	Lung	Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.	Lungs
D008297	Male		Males
D009532	Nickel	A trace element with the atomic symbol Ni, atomic number 28, and atomic weight 58.69. It is a cofactor of the enzyme UREASE.
D001992	Bronchoalveolar Lavage Fluid	Washing liquid obtained from irrigation of the lung, including the BRONCHI and the PULMONARY ALVEOLI. It is generally used to assess biochemical, inflammatory, or infection status of the lung.	Alveolar Lavage Fluid,Bronchial Lavage Fluid,Lung Lavage Fluid,Bronchial Alveolar Lavage Fluid,Lavage Fluid, Bronchial,Lavage Fluid, Lung,Pulmonary Lavage Fluid,Alveolar Lavage Fluids,Bronchial Lavage Fluids,Bronchoalveolar Lavage Fluids,Lavage Fluid, Alveolar,Lavage Fluid, Bronchoalveolar,Lavage Fluid, Pulmonary,Lavage Fluids, Alveolar,Lavage Fluids, Bronchial,Lavage Fluids, Bronchoalveolar,Lavage Fluids, Lung,Lavage Fluids, Pulmonary,Lung Lavage Fluids,Pulmonary Lavage Fluids
D000280	Administration, Inhalation	The administration of drugs by the respiratory route. It includes insufflation into the respiratory tract.	Drug Administration, Inhalation,Drug Administration, Respiratory,Drug Aerosol Therapy,Inhalation Drug Administration,Inhalation of Drugs,Respiratory Drug Administration,Aerosol Drug Therapy,Aerosol Therapy, Drug,Drug Therapy, Aerosol,Inhalation Administration,Administration, Inhalation Drug,Administration, Respiratory Drug,Therapy, Aerosol Drug,Therapy, Drug Aerosol
D000393	Air Pollutants	Any substance in the air which could, if present in high enough concentration, harm humans, animals, vegetation or materials. Substances include GASES; PARTICULATE MATTER; and volatile ORGANIC CHEMICALS.	Air Pollutant,Air Pollutants, Environmental,Environmental Air Pollutants,Environmental Pollutants, Air,Air Environmental Pollutants,Pollutant, Air,Pollutants, Air,Pollutants, Air Environmental,Pollutants, Environmental Air
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D017208	Rats, Wistar	A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.	Wistar Rat,Rat, Wistar,Wistar Rats