In multispectral imaging, Wiener estimation is widely adopted for the reconstruction of spectral reflectance. We propose an improved reflectance reconstruction method by adaptively selecting training samples for the autocorrelation matrix calculation in Wiener estimation, without a prior knowledge of the spectral information of the samples being imaged. The performance of the proposed adaptive Wiener estimation and the traditional method are compared in the cases of different channel numbers and noise levels. Experimental results show that the proposed method outperforms the traditional method in terms of both spectral and colorimetric prediction errors when the imaging channel number is 7 or less. When the imaging system consists of 11 or more channels, the color accuracy of the proposed method is slightly better than or becomes close to that of the traditional method.