BIOMAT Database Logo BIOMAT Database Logo

Detection of respiratory syncytial virus antigen and nucleic acid in clinical specimens using synthetic oligonucleotides. 1991

H A Cubie, and J M Inglis, and A M McGowan
Regional Virus Laboratory, City Hospital, Edinburgh, U.K.

Rapid detection of respiratory syncytial (RS) virus in nasopharyngeal secretions (NPS) was carried out on cytospin cell preparations using a directly labelled monoclonal antiserum to RS virus to detect viral antigen and digoxigenin-labelled synthetic oligonucleotides to detect viral nucleic acid. Sequences of 27 and 30 bases in length from within the fusion protein and nucleocapsid genes respectively were selected for use as probes. The oligonucleotide in situ hybridization test was easy to perform and could be completed within 24 hours, but antigen detection was much more rapid and more sensitive. During 1989-1990, more positive results were obtained by antigen detection (193) than by isolation (185), but of 43 confirmed RS-virus-positive specimens, only 21 (49%) were detected by ISH. Antigen detection remains the most suitable single method of rapid detection of RS virus for a diagnostic laboratory.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D012136 Respiratory Syncytial Viruses A group of viruses in the PNEUMOVIRUS genus causing respiratory infections in various mammals. Humans and cattle are most affected but infections in goats and sheep have also been reported. Chimpanzee Coryza Agent,Orthopneumovirus,RSV Respiratory Syncytial Virus,Chimpanzee Coryza Agents,Coryza Agent, Chimpanzee,Orthopneumoviruses,Respiratory Syncytial Virus,Syncytial Virus, Respiratory,Virus, Respiratory Syncytial
D004279 DNA, Viral Deoxyribonucleic acid that makes up the genetic material of viruses. Viral DNA
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000956 Antigens, Viral Substances elaborated by viruses that have antigenic activity. Viral Antigen,Viral Antigens,Antigen, Viral
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D015345 Oligonucleotide Probes Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin. Oligodeoxyribonucleotide Probes,Oligonucleotide Probe,Oligoribonucleotide Probes,Probe, Oligonucleotide,Probes, Oligodeoxyribonucleotide,Probes, Oligonucleotide,Probes, Oligoribonucleotide

Related Publications

H A Cubie, and J M Inglis, and A M McGowan
Rapid detection of respiratory syncytial virus in clinical specimens.
September 1987, Acta virologica,
H A Cubie, and J M Inglis, and A M McGowan
Comparison of direct and indirect immunofluorescence staining of clinical specimens for detection of respiratory syncytial virus antigen.
May 1982, Journal of clinical microbiology,
H A Cubie, and J M Inglis, and A M McGowan
Application of pooled monoclonal antibodies for 1-hr detection of respiratory syncytial virus antigen in clinical specimens.
June 1987, Diagnostic microbiology and infectious disease,
H A Cubie, and J M Inglis, and A M McGowan
[Respiratory syncytial virus: rapid detection of antigen using monoclonal antibodies].
November 1985, Nederlands tijdschrift voor geneeskunde,
H A Cubie, and J M Inglis, and A M McGowan
[Detection of respiratory syncytial virus antigen in respiratory secretions].
January 1993, Enfermedades infecciosas y microbiologia clinica,
H A Cubie, and J M Inglis, and A M McGowan
["Detection of respiratory syncytial virus antigen in respiratory secretions"].
January 1994, Enfermedades infecciosas y microbiologia clinica,
H A Cubie, and J M Inglis, and A M McGowan
Synthetic oligonucleotide probes differentiate respiratory syncytial virus subgroups in a nucleic acid hybridization assay.
June 1991, Journal of clinical microbiology,
H A Cubie, and J M Inglis, and A M McGowan
Detection of respiratory syncytial virus nucleic acid in archival postmortem tissue from infants.
January 1997, Pediatric pathology & laboratory medicine : journal of the Society for Pediatric Pathology, affiliated with the International Paediatric Pathology Association,
H A Cubie, and J M Inglis, and A M McGowan
Bispecific Antibody Detection Using Antigen-Conjugated Synthetic Nucleic Acid Strands.
November 2023, ACS sensors,
H A Cubie, and J M Inglis, and A M McGowan
Comparison of Six Sample-to-Answer Influenza A/B and Respiratory Syncytial Virus Nucleic Acid Amplification Assays Using Respiratory Specimens from Children.
November 2018, Journal of clinical microbiology,
Copied contents to your clipboard!