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The effects of sodium cyanide (NaCN) on the gamma-aminobutyric acid metabolizing enzymes glutamic acid decarboxylase (GAD) and gamma-aminobutyric acid transaminase (GABA-T) were studied in vitro. With no pyridoxal-5-phosphate added, GAD was non-competitively inhibited by NaCN, with an IC50 of 280 microM. GAD was also inhibited when exposed to an equimolar amount of NaCN and pyridoxal-5-phosphate. NaCN inhibited GABA-T. The inhibition kinetics suggests that NaCN may react with more than one of the substrates and products present during the reaction, i.e. pyridoxal-5-phosphate, alpha-ketoglutarate and/or succinic semialdehyde. The presence of pyridoxal-5-phosphate in the reaction mixture completely protected GABA-T from inhibition by NaCN. The gamma-aminobutyric acid synthesizing enzyme, GAD may thus be inhibited in vivo by NaCN or by a reaction product of NaCN and pyridoxal-5-phosphate. The gamma-aminobutyric acid catabolizing enzyme, GABA-T is not as vulnerable to inhibition by NaCN, since the cyanide-pyridoxal-5-phosphate complex is ineffective as inhibitor.
G Cassel, and
L Karlsson, and
A Sellström
June 1962,
Bratislavske lekarske listy,
G Cassel, and
L Karlsson, and
A Sellström
February 1963,
Life sciences (1962),
G Cassel, and
L Karlsson, and
A Sellström
January 1968,
Acta biologica et medica Germanica,
G Cassel, and
L Karlsson, and
A Sellström
August 1998,
Annals of neurology,
G Cassel, and
L Karlsson, and
A Sellström
November 1969,
Nature,
G Cassel, and
L Karlsson, and
A Sellström
March 1964,
Canadian journal of physiology and pharmacology,
G Cassel, and
L Karlsson, and
A Sellström
September 1963,
Sheng li xue bao : [Acta physiologica Sinica],
G Cassel, and
L Karlsson, and
A Sellström
June 1960,
Biochimica et biophysica acta,
G Cassel, and
L Karlsson, and
A Sellström
December 1965,
Archives internationales de pharmacodynamie et de therapie,
G Cassel, and
L Karlsson, and
A Sellström
March 1988,
Experimental neurology,
