This study analyzed if the running speed corresponding to glucose minimum (GM) could predict the maximal lactate steady state (MLSS). Thirteen physically active men (25.2+/-4.2 years, 73.4+/-8.0 kg, 180.0+/-1.0 cm) completed three running tests on different days: 1) a 1 600-m time trial to calculate the average speed; 2) after 10-min of recovery from a 150-m sprint to elevate [lac], participants performed 6 series of 800-m respectively at 78, 81, 84, 87, 90 and 93% of the 1 600-m speed to identify the lactate minimum (LM) and GM speeds and 3) 2-4 constant intensity exercise sessions for the MLSS. Repeated measures ANOVA showed no differences between running speeds associated to the GM (201.7+/-23.8 m.min (-1)), LM (200.0+/-23.9 m.min (-1)) and MLSS (201.5+/-23.1 m.min (-1)), with high correlation between GM vs. LM (r=0.984), GM vs. MLSS (r=0.947) and LM vs. MLSS (r=0.961) (P<0.01). Bland and Altman plots showed good agreement [Bias (+/-95% CI)] for MLSS and GM [0.2(15.3) m.min (-1)], MLSS and LM [-1.4(13.2) m.min (-1)], as well as for LM and GM [1.7(8.5) m.min (-1)]. These running speeds occurred at approximately 84.4% of 1 600-m speed, which would have practical applications for exercise prescription. We concluded that GM running speed is a good predictor of the MLSS for physically active individuals.