Biomaterial Database

Relationship between MVD and meshed-capillaries using magnifying NBI colonoscopy in colorectal precursor lesions. 2009

Takahiro Horimatsu, and Yasushi Sano, and Kazuhiro Kaneko, and Hiroaki Ikematsu, and Atushi Katagiri, and Tomonori Yano, and Kuang I Fu, and Manabu Muto, and Satoshi Fujii, and Atsushi Ochiai, and Shigeaki Yoshida

Division of Digestive Endoscopy/Gastrointestinal Oncology, National Cancer Centre Hospital East, Chiba, Japan.

OBJECTIVE To prospectively investigate differences in angiogenesis between low-grade dysplasia (LGD) and hyperplastic polyps, as well as the relationship between microvessel density (MVD) as identified by histological analysis and meshed-capillary (MC) vessels visualized using magnifying narrow band imaging (NBI) colonoscopy. METHODS Immunohistochemical analysis of MVD was performed using monoclonal antibody against CD31. The number and thickness of vessels were investigated for 45 LGD and 25 hyperplastic polyps. In contrast, NBI colonoscopy was able to classify all lesions into either visualization or nonvisualization of MC vessels. RESULTS No significant difference was seen in the number of thin vessels (< or =10 microm) using immunohistochemical MVD analysis between LGDand hyperplasic polyps (p = 0.36). In contrast, the number of intermediate (11-20 microm) and thick vessels (> or =21 microm) differed significantly between LGD and hyperplasic polyps, respectively (p < 0.0001, p < 0.0001). In NBI findings, the presence of MC vessels was found in 98% (44 of 45) of LGD, while MC vessels were absent in 92% (23 of 25) of hyperplasic polyps. CONCLUSIONS We postulated that the incidence of intermediate and thick vessels (>10 microm) is related to angiogenesis occurring during carcinogenesis of colorectal mucosa. Furthermore, the appearance of visualized MC vessels during magnifying NBI colonoscopy can be used to identify angiogenesis in colorectal LGD and hyperplastic polyps.

UI	MeSH Term	Description	Entries
D007124	Immunoenzyme Techniques	Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.	Antibody Enzyme Technique, Unlabeled,Enzyme Immunoassay,Enzyme-Labeled Antibody Technique,Immunoassay, Enzyme,Immunoperoxidase Techniques,Peroxidase-Antiperoxidase Complex Technique,Peroxidase-Labeled Antibody Technique,Antibody Enzyme Technic, Unlabeled,Enzyme-Labeled Antibody Technic,Immunoenzyme Technics,Immunoperoxidase Technics,Peroxidase-Antiperoxidase Complex Technic,Peroxidase-Labeled Antibody Technic,Antibody Technic, Enzyme-Labeled,Antibody Technic, Peroxidase-Labeled,Antibody Technics, Enzyme-Labeled,Antibody Technics, Peroxidase-Labeled,Antibody Technique, Enzyme-Labeled,Antibody Technique, Peroxidase-Labeled,Antibody Techniques, Enzyme-Labeled,Antibody Techniques, Peroxidase-Labeled,Enzyme Immunoassays,Enzyme Labeled Antibody Technic,Enzyme Labeled Antibody Technique,Enzyme-Labeled Antibody Technics,Enzyme-Labeled Antibody Techniques,Immunoassays, Enzyme,Immunoenzyme Technic,Immunoenzyme Technique,Immunoperoxidase Technic,Immunoperoxidase Technique,Peroxidase Antiperoxidase Complex Technic,Peroxidase Antiperoxidase Complex Technique,Peroxidase Labeled Antibody Technic,Peroxidase Labeled Antibody Technique,Peroxidase-Antiperoxidase Complex Technics,Peroxidase-Antiperoxidase Complex Techniques,Peroxidase-Labeled Antibody Technics,Peroxidase-Labeled Antibody Techniques,Technic, Enzyme-Labeled Antibody,Technic, Immunoenzyme,Technic, Immunoperoxidase,Technic, Peroxidase-Antiperoxidase Complex,Technic, Peroxidase-Labeled Antibody,Technics, Enzyme-Labeled Antibody,Technics, Immunoenzyme,Technics, Immunoperoxidase,Technics, Peroxidase-Antiperoxidase Complex,Technics, Peroxidase-Labeled Antibody,Technique, Enzyme-Labeled Antibody,Technique, Immunoenzyme,Technique, Immunoperoxidase,Technique, Peroxidase-Antiperoxidase Complex,Technique, Peroxidase-Labeled Antibody,Techniques, Enzyme-Labeled Antibody,Techniques, Immunoenzyme,Techniques, Immunoperoxidase,Techniques, Peroxidase-Antiperoxidase Complex,Techniques, Peroxidase-Labeled Antibody
D008297	Male		Males
D008875	Middle Aged	An adult aged 45 - 64 years.	Middle Age
D009389	Neovascularization, Pathologic	A pathologic process consisting of the proliferation of blood vessels in abnormal tissues or in abnormal positions.	Angiogenesis, Pathologic,Angiogenesis, Pathological,Neovascularization, Pathological,Pathologic Angiogenesis,Pathologic Neovascularization,Pathological Angiogenesis,Pathological Neovascularization
D011230	Precancerous Conditions	Pathological conditions that tend eventually to become malignant.	Preneoplastic Conditions,Condition, Preneoplastic,Conditions, Preneoplastic,Preneoplastic Condition,Condition, Precancerous,Conditions, Precancerous,Precancerous Condition
D011446	Prospective Studies	Observation of a population for a sufficient number of persons over a sufficient number of years to generate incidence or mortality rates subsequent to the selection of the study group.	Prospective Study,Studies, Prospective,Study, Prospective
D003111	Colonic Polyps	Discrete tissue masses that protrude into the lumen of the COLON. These POLYPS are connected to the wall of the colon either by a stalk, pedunculus, or by a broad base.	Colonic Polyp,Polyp, Colonic,Polyps, Colonic
D003113	Colonoscopy	Endoscopic examination, therapy or surgery of the luminal surface of the colon.	Colonoscopic Surgical Procedures,Surgical Procedures, Colonoscopic,Colonoscopic Surgery,Surgery, Colonoscopic,Colonoscopic Surgeries,Colonoscopic Surgical Procedure,Colonoscopies,Procedure, Colonoscopic Surgical,Procedures, Colonoscopic Surgical,Surgeries, Colonoscopic,Surgical Procedure, Colonoscopic
D005260	Female		Females
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man