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The effects of blinding alone or in combination with pinealectomy, castration and melatonin treatment on nerve growth factor (NGF) in submaxillary glands of mice were tested. Both pinealectomy and melatonin (placed subcutaneously in beeswax) treatment caused a marked reduction in NGF specific biological activity, esterase activity, and beta-protein recovered from the submaxillary glands. Castrated mice also exhibited depressed levels of NGF in the salivary glands.
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