BIOMAT Database Logo BIOMAT Database Logo

Detection of amphetamine and methamphetamine in urine by gas chromatography/mass spectrometry following derivatization with (-)-menthyl chloroformate. 1991

R O Hughes, and W E Bronner, and M L Smith
Office of the Armed Forces Medical Examiner, Armed Forces Institute of Pathology, Washington, DC 20306-6000.

A gas chromatography/mass spectrometry assay was developed for the detection of amphetamine and methamphetamine in urine. These drugs were detected as carbamate derivatives following reaction with (-)-menthyl chloroformate. Selected ion monitoring of the 226, 182, and 227 dalton ions for amphetamine and the 240, 102, and 196 dalton ions for methamphetamine provided positive analyte identification. Quantitative values obtained by isotope dilution mass spectrometry displayed a linear response from below 50 to above 6,000 ng/mL. Differentiation of over-the-counter (R)-methamphetamine from illicit (S)-methamphetamine was accomplished by chromatographic separation of the diastereomeric derivatives on an achiral column. No interferences were observed from ephedrine, pseudoephedrine, phentermine, phenylpropanolamine, or 2-phenylethylamine.

UI MeSH Term Description Entries
D008401 Gas Chromatography-Mass Spectrometry A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds. Chromatography, Gas-Liquid-Mass Spectrometry,Chromatography, Gas-Mass Spectrometry,GCMS,Spectrometry, Mass-Gas Chromatography,Spectrum Analysis, Mass-Gas Chromatography,Gas-Liquid Chromatography-Mass Spectrometry,Mass Spectrometry-Gas Chromatography,Chromatography, Gas Liquid Mass Spectrometry,Chromatography, Gas Mass Spectrometry,Chromatography, Mass Spectrometry-Gas,Chromatography-Mass Spectrometry, Gas,Chromatography-Mass Spectrometry, Gas-Liquid,Gas Chromatography Mass Spectrometry,Gas Liquid Chromatography Mass Spectrometry,Mass Spectrometry Gas Chromatography,Spectrometries, Mass-Gas Chromatography,Spectrometry, Gas Chromatography-Mass,Spectrometry, Gas-Liquid Chromatography-Mass,Spectrometry, Mass Gas Chromatography,Spectrometry-Gas Chromatography, Mass,Spectrum Analysis, Mass Gas Chromatography
D008694 Methamphetamine A central nervous system stimulant and sympathomimetic with actions and uses similar to DEXTROAMPHETAMINE. The smokable form is a drug of abuse and is referred to as crank, crystal, crystal meth, ice, and speed. Deoxyephedrine,Desoxyephedrine,Desoxyn,Madrine,Metamfetamine,Methamphetamine Hydrochloride,Methylamphetamine,N-Methylamphetamine,Hydrochloride, Methamphetamine,N Methylamphetamine
D005561 Formates Derivatives of formic acids. Included under this heading are a broad variety of acid forms, salts, esters, and amides that are formed with a single carbon carboxy group. Formic Acids,Acids, Formic
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000661 Amphetamine A powerful central nervous system stimulant and sympathomimetic. Amphetamine has multiple mechanisms of action including blocking uptake of adrenergics and dopamine, stimulation of release of monamines, and inhibiting monoamine oxidase. Amphetamine is also a drug of abuse and a psychotomimetic. The l- and the d,l-forms are included here. The l-form has less central nervous system activity but stronger cardiovascular effects. The d-form is DEXTROAMPHETAMINE. Desoxynorephedrin,Levoamphetamine,Phenopromin,l-Amphetamine,Amfetamine,Amphetamine Sulfate,Amphetamine Sulfate (2:1),Centramina,Fenamine,Mydrial,Phenamine,Thyramine,levo-Amphetamine,Sulfate, Amphetamine,l Amphetamine,levo Amphetamine

Related Publications

R O Hughes, and W E Bronner, and M L Smith
Determination of amphetamine and methamphetamine in blood by derivatization with perfluorooctanoyl chloride and gas chromatography/mass spectrometry.
January 1993, Journal of analytical toxicology,
R O Hughes, and W E Bronner, and M L Smith
Determination of thiazolidine-4-carboxylates in urine by chloroformate derivatization and gas chromatography-electron impact mass spectrometry.
September 2007, Journal of mass spectrometry : JMS,
R O Hughes, and W E Bronner, and M L Smith
Distinguishing sympathomimetic amines from amphetamine and methamphetamine in urine by gas chromatography/mass spectrometry.
January 1992, Journal of analytical toxicology,
R O Hughes, and W E Bronner, and M L Smith
Gas chromatography/mass spectrometry determination of amphetamine-related drugs and ephedrines in plasma, urine and hair samples after derivatization with 2,2,2-trichloroethyl chloroformate.
January 2005, Rapid communications in mass spectrometry : RCM,
R O Hughes, and W E Bronner, and M L Smith
On-column derivatization for determination of amphetamine and methamphetamine in human blood by gas chromatography-mass spectrometry.
February 2002, Forensic science international,
R O Hughes, and W E Bronner, and M L Smith
Analysis of dencichine in Panax notoginseng by gas chromatography-mass spectrometry with ethyl chloroformate derivatization.
February 2007, Journal of pharmaceutical and biomedical analysis,
R O Hughes, and W E Bronner, and M L Smith
Detection of amphetamine and related amines in urine by gas chromatography and combined gas chromatography- mass spectrometry.
September 1973, Chemical & pharmaceutical bulletin,
R O Hughes, and W E Bronner, and M L Smith
Routine analysis of amphetamine and methamphetamine in biological materials by gas chromatography-mass spectrometry and on-column derivatization.
June 2003, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
R O Hughes, and W E Bronner, and M L Smith
Metabolite analysis of human fecal water by gas chromatography/mass spectrometry with ethyl chloroformate derivatization.
October 2009, Analytical biochemistry,
R O Hughes, and W E Bronner, and M L Smith
Simultaneous analysis of amphetamine, methamphetamine, and 3, 4-methylenedioxymethamphetamine (MDMA) in urine samples by solid-phase extraction, derivatization, and gas chromatography/mass spectrometry.
September 1991, Journal of forensic sciences,
Copied contents to your clipboard!