Toxoplasma gondii: Evidence for the transmission by semen in dogs. 2009

Tiago Pereira Arantes, and Welber Daniel Zanetti Lopes, and Roberta Machado Ferreira, and Juliana S Pinto Pieroni, and Vanessa M R Pinto, and Claudio A Sakamoto, and Alvimar José da Costa
CPPAR - Animal Health Research Center, UNESP, Jaboticabal, São Paulo, Brazil. tiago.arantes@yahoo.com.br

Ten male dogs were distributed into three experimental groups for infection with Toxoplasma gondii: GI - three dogs inoculated with 2.0x10(5) P strais oocysts, GII - three dogs infected with 1.0x10(6) RH strain tachyzoites, and GIII - four controls dogs. Several clinical parameters were evaluated. IFAT was performed to detect anti-T. gondii antibodies. Presence of the parasite in semen was evaluated by PCR and bioassay techniques. Tissue parasitism was examined using bioassays and immunohistochemistry in testicle and epididymis fragments collected after orchiectomy. In semen samples collected from these two groups, the presence of T. gondii was verified by bioassays and PCR. T. gondii was detected by immunohistochemistry in tissues (testicle and epididymis fragments) of all six experimentally infected dogs. The T. gondii-positive seminal samples were used in the artificial insemination (AI) of four female dogs free of toxoplasmic infection. Seven days after AI, all of the female dogs presented serologic conversion (IFAT). Fetal reabsorption occurred in two of the dogs, while the others sustained full-term gestation. Several T. gondii cysts were detected in the brains of four offspring. These results suggest that T. gondii can be sexually transmitted in domestic dogs.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D007315 Insemination, Artificial Artificial introduction of SEMEN or SPERMATOZOA into the VAGINA to facilitate FERTILIZATION. Artificial Insemination,Eutelegenesis,Artificial Inseminations,Eutelegeneses,Inseminations, Artificial
D008297 Male Males
D011897 Random Allocation A process involving chance used in therapeutic trials or other research endeavor for allocating experimental subjects, human or animal, between treatment and control groups, or among treatment groups. It may also apply to experiments on inanimate objects. Randomization,Allocation, Random
D004283 Dog Diseases Diseases of the domestic dog (Canis familiaris). This term does not include diseases of wild dogs, WOLVES; FOXES; and other Canidae for which the heading CARNIVORA is used. Canine Diseases,Canine Disease,Disease, Canine,Disease, Dog,Diseases, Canine,Diseases, Dog,Dog Disease
D004285 Dogs The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065) Canis familiaris,Dog
D005260 Female Females
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001681 Biological Assay A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc. Bioassay,Assay, Biological,Assays, Biological,Biologic Assay,Biologic Assays,Assay, Biologic,Assays, Biologic,Bioassays,Biological Assays
D012661 Semen The thick, yellowish-white, viscid fluid secretion of male reproductive organs discharged upon ejaculation. In addition to reproductive organ secretions, it contains SPERMATOZOA and their nutrient plasma. Seminal Plasma,Plasma, Seminal

Related Publications

Tiago Pereira Arantes, and Welber Daniel Zanetti Lopes, and Roberta Machado Ferreira, and Juliana S Pinto Pieroni, and Vanessa M R Pinto, and Claudio A Sakamoto, and Alvimar José da Costa
June 2006, Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases,
Tiago Pereira Arantes, and Welber Daniel Zanetti Lopes, and Roberta Machado Ferreira, and Juliana S Pinto Pieroni, and Vanessa M R Pinto, and Claudio A Sakamoto, and Alvimar José da Costa
December 1997, Veterinary parasitology,
Tiago Pereira Arantes, and Welber Daniel Zanetti Lopes, and Roberta Machado Ferreira, and Juliana S Pinto Pieroni, and Vanessa M R Pinto, and Claudio A Sakamoto, and Alvimar José da Costa
March 2017, Theriogenology,
Tiago Pereira Arantes, and Welber Daniel Zanetti Lopes, and Roberta Machado Ferreira, and Juliana S Pinto Pieroni, and Vanessa M R Pinto, and Claudio A Sakamoto, and Alvimar José da Costa
December 2009, Parasitology,
Tiago Pereira Arantes, and Welber Daniel Zanetti Lopes, and Roberta Machado Ferreira, and Juliana S Pinto Pieroni, and Vanessa M R Pinto, and Claudio A Sakamoto, and Alvimar José da Costa
January 1978, The Veterinary record,
Tiago Pereira Arantes, and Welber Daniel Zanetti Lopes, and Roberta Machado Ferreira, and Juliana S Pinto Pieroni, and Vanessa M R Pinto, and Claudio A Sakamoto, and Alvimar José da Costa
January 2010, Advances in food and nutrition research,
Tiago Pereira Arantes, and Welber Daniel Zanetti Lopes, and Roberta Machado Ferreira, and Juliana S Pinto Pieroni, and Vanessa M R Pinto, and Claudio A Sakamoto, and Alvimar José da Costa
November 1972, The Journal of infectious diseases,
Tiago Pereira Arantes, and Welber Daniel Zanetti Lopes, and Roberta Machado Ferreira, and Juliana S Pinto Pieroni, and Vanessa M R Pinto, and Claudio A Sakamoto, and Alvimar José da Costa
January 1968, Acta pathologica et microbiologica Scandinavica,
Tiago Pereira Arantes, and Welber Daniel Zanetti Lopes, and Roberta Machado Ferreira, and Juliana S Pinto Pieroni, and Vanessa M R Pinto, and Claudio A Sakamoto, and Alvimar José da Costa
January 1967, Transactions of the Royal Society of Tropical Medicine and Hygiene,
Tiago Pereira Arantes, and Welber Daniel Zanetti Lopes, and Roberta Machado Ferreira, and Juliana S Pinto Pieroni, and Vanessa M R Pinto, and Claudio A Sakamoto, and Alvimar José da Costa
February 1974, Bulletin of the New York Academy of Medicine,
Copied contents to your clipboard!