Biomaterial Database

The expression of vascular endothelial growth factor in skeletal muscle of patients with sleep disorders. 2009

Britta Wåhlin-Larsson, and Jan Ulfberg, and Karin Piehl Aulin, and Fawzi Kadi

School of Health and Medical Sciences, Orebro University, Orebro, Sweden. bwl@du.se

An increased capillary network has been observed in skeletal muscle of patients with restless legs syndrome (RLS) and obstructive sleep apnea syndrome (OSAS). This finding could be due to upregulation of growth factors responsible for angiogenesis. The aim of the study was to examine the occurrence and localization of VEGF and capillary proliferation in skeletal muscle of RLS (n = 12) and OSAS (n = 12) patients and controls (n = 11). Double-immunofluorescence staining for capillaries (CD31) and VEGF, and proliferating cells (Ki-67), was carried out on biopsies taken from the tibialis anterior. The percentage of capillaries expressing VEGF (CD31,VEGF(+)) was significantly higher in OSAS and RLS patients compared with controls. The percentage of proliferating capillaries (CD31,Ki-67(+)) was significantly higher in OSAS patients compared with controls. Our study shows the occurrence of proliferation of endothelial cells in skeletal muscle in RLS and OSAS, supporting an upregulation of VEGF located in capillaries, probably due to local hypoxia.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D008875	Middle Aged	An adult aged 45 - 64 years.	Middle Age
D012016	Reference Values	The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality.	Normal Range,Normal Values,Reference Ranges,Normal Ranges,Normal Value,Range, Normal,Range, Reference,Ranges, Normal,Ranges, Reference,Reference Range,Reference Value,Value, Normal,Value, Reference,Values, Normal,Values, Reference
D012148	Restless Legs Syndrome	A disorder characterized by aching or burning sensations in the lower and rarely the upper extremities that occur prior to sleep or may awaken the patient from sleep.	Restless Leg Syndrome,Restless Legs,Willis Ekbom Disease,Willis Ekbom Syndrome,Willis-Ekbom Disease,Willis-Ekbom Syndrome,Wittmaack Ekbom Syndrome,Wittmaack-Ekbom Syndrome,Disease, Willis Ekbom,Disease, Willis-Ekbom,Syndrome, Restless Leg,Syndrome, Willis Ekbom,Syndrome, Willis-Ekbom,Syndrome, Wittmaack Ekbom,Syndrome, Wittmaack-Ekbom
D002196	Capillaries	The minute vessels that connect arterioles and venules.	Capillary Beds,Sinusoidal Beds,Sinusoids,Bed, Sinusoidal,Beds, Sinusoidal,Capillary,Capillary Bed,Sinusoid,Sinusoidal Bed
D005260	Female		Females
D005455	Fluorescent Antibody Technique	Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.	Antinuclear Antibody Test, Fluorescent,Coon's Technique,Fluorescent Antinuclear Antibody Test,Fluorescent Protein Tracing,Immunofluorescence Technique,Coon's Technic,Fluorescent Antibody Technic,Immunofluorescence,Immunofluorescence Technic,Antibody Technic, Fluorescent,Antibody Technics, Fluorescent,Antibody Technique, Fluorescent,Antibody Techniques, Fluorescent,Coon Technic,Coon Technique,Coons Technic,Coons Technique,Fluorescent Antibody Technics,Fluorescent Antibody Techniques,Fluorescent Protein Tracings,Immunofluorescence Technics,Immunofluorescence Techniques,Protein Tracing, Fluorescent,Protein Tracings, Fluorescent,Technic, Coon's,Technic, Fluorescent Antibody,Technic, Immunofluorescence,Technics, Fluorescent Antibody,Technics, Immunofluorescence,Technique, Coon's,Technique, Fluorescent Antibody,Technique, Immunofluorescence,Techniques, Fluorescent Antibody,Techniques, Immunofluorescence,Tracing, Fluorescent Protein,Tracings, Fluorescent Protein
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328	Adult	A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available.	Adults
D001706	Biopsy	Removal and pathologic examination of specimens from the living body.	Biopsies