[Determination of avermectin residues in tea by ultra-performance liquid chromatography-electrospray tandem mass spectrometry]. 2009

Fang Yang, and Shoushen Yang, and Yonghui Lin, and Danping Zheng, and Shengyu Lu, and Zhiqiang Huang, and Ying Zhang
Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou 350001, China. yffjciq@gmail.com

A simple and rapid analytical method for the simultaneous determination of abamectin, emamectin, eprinomectin, ivermectin, doramectin and moxidectin residues in tea by ultra-performance liquid chromatography-electrospray tandem mass spectrometry (UPLC/ESI-MS/MS) has been developed. The avermectins were extracted from the tea with acetonitrile after the tea was infiltrated in saturated aqueous NaCl solution, then cleaned up with a C18 solid phase extraction cartridge. The linear ranges were 2.0-50 microg/L and the correlation coefficients were all above 0.9920. Several UPLC-MS/MS conditions that included the mobile phase, monitor ions and the selection of calibration of the measurement were studied. The average recoveries and the relative standard deviations ranged from 61.7% to 85.4% and from 9.37% to 17.19%, respectively, in spiked samples at the concentrations of 5, 10, 20 microg/kg for moxidectin and 2, 5, 10 microg/kg for other analytes. This method is suitable for the determination of avermeetin residues in tea.

UI MeSH Term Description Entries
D007559 Ivermectin A mixture of mostly avermectin H2B1a (RN 71827-03-7) with some avermectin H2B1b (RN 70209-81-3), which are macrolides from STREPTOMYCES avermitilis. It binds glutamate-gated chloride channel to cause increased permeability and hyperpolarization of nerve and muscle cells. It also interacts with other CHLORIDE CHANNELS. It is a broad spectrum antiparasitic that is active against microfilariae of ONCHOCERCA VOLVULUS but not the adult form. Eqvalan,Ivomec,MK-933,Mectizan,Stromectol,MK 933,MK933
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D004187 Disaccharides Oligosaccharides containing two monosaccharide units linked by a glycosidic bond. Disaccharide
D004350 Drug Residues Drugs and their metabolites which are found in the edible tissues and milk of animals after their medication with specific drugs. This term can also apply to drugs found in adipose tissue of humans after drug treatment. Drug Residue,Residue, Drug,Residues, Drug
D005506 Food Contamination The presence in food of harmful, unpalatable, or otherwise objectionable foreign substances, e.g. chemicals, microorganisms or diluents, before, during, or after processing or storage. Food Adulteration,Adulteration, Food,Adulterations, Food,Contamination, Food,Contaminations, Food,Food Adulterations,Food Contaminations
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013662 Tea The infusion of leaves of CAMELLIA SINENSIS (formerly Thea sinensis) as a beverage, the familiar Asian tea, which contains CATECHIN (especially epigallocatechin gallate) and CAFFEINE. Black Tea,Green Tea,Black Teas,Green Teas,Tea, Black,Tea, Green,Teas, Black,Teas, Green
D053719 Tandem Mass Spectrometry A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection. Mass Spectrometry-Mass Spectrometry,Mass Spectrometry Mass Spectrometry,Mass Spectrometry, Tandem

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